Two dimensional electrophoresis protocols / edited by David Sheehan, Raymond Tyther.

Contributor(s): Sheehan, David, 1958- | Tyther, Raymond
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 519.; Springer protocols (Series): Publisher: New York : Humana Press, ©2009Description: 1 online resource (xiv, 545 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781597452816; 1597452815Subject(s): Two-dimensional electrophoresis | Electrophoresis, Gel, Two-Dimensional -- methods | Two-dimensional electrophoresis | Animal Biochemistry | Biochemistry | Human Anatomy & Physiology | Chemistry | Health & Biological Sciences | Physical Sciences & Mathematics | elektroforese | electrophoresis | eiwitten | proteins | ph | immunoblotting | eiwitexpressieanalyse | proteomics | Analytical Chemistry | Analytische chemieGenre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Two-dimensional electrophoresis protocols.DDC classification: 572.36 LOC classification: QP519.9.T84Online resources: Click here to access online
Contents:
Two-dimensional electrophoresis : an overview / Richard Smith -- Solubilization of proteins in 2DE : an outline / Thierry Rabilloud -- Selection of pH ranges in 2DE / Mireille Starita-Geribaldi -- Difficult proteins / Ben Herbert and Elizabeth Harry -- Organelle proteomics / Matthias Plöscher [and others] -- Applications of chemical tagging approaches in combination with 2DE and mass spectrometry / Alexander Leitner and Wolfgang Lindner -- Immunoblotting 2DE membranes / Brian McDonagh -- Troubleshooting image analysis in 2DE / Bettina Levänen and Åsa M. Wheelock -- Analysis of bacterial proteins by 2DE / Philip Cash and Evelyn Argo -- Proteomic analysis of Caenorhabditis elegans / Pan-Young Jeong [and others] -- Protein extraction for 2DE / Claus Zabel and Joachim Klose -- Analysis of proteins from marine molluscs / Suze Chora, Maria João Bebianno, and Michèle Roméo -- Preparation and analysis of plastid proteomes by 2DE / Anne von Zychlinski and Wilhelm Gruissem -- High resolution 2DE / Katrin Marcus [and others] -- Blue native-gel electrophoresis proteomics / Kelly Andringa, Adrienne King, and Shannon Bailey -- 2DE for proteome analysis of human metaphase chromosomes / Kiichi Fukui and Susumu Uchiyama -- Microsomal proteomics / Diana M. Wong and Khosrow Adeli -- Prefractionation using microscale solution IEF / Won-A Joo and David Speicher -- Diagonal electrophoresis for detection of protein disulphide bridges / Brian McDonagh -- High-resolution large-gel 2DE / Claus Zabel and Joachim Klose -- Silver staining of proteins in 2DE gels / Cécile Lelong [and others] -- Detection of 4-hydroxy-2-nonenal- and 3-nitrotyrosine-modified proteins using a proteomics approach / Rukhsana Sultana, Tanea Reed, and D. Allan Butterfield -- Proteomic detection of oxidized and reduced thiol proteins in cultured cells / Sarah L. Cuddihy [and others] -- Detection of ubiquitination in 2DE / Brian McDonagh -- Phosphoroproteome analysis by in-gel isoelectric focusing and tandem mass spectrometry / Sarka Beranova-Georgianni, Dominic M. Desiderio, and Francesco Giorgianni -- Detection of protein glutathionylation / Elisabetta Gianazza, Ivano Eberini, and Pietro Ghezzi -- Activity-based protein profiling of protein tyrosine phosphatases / Chad Walls, Bo Zhou, and Zhong-Yin Zhang -- Active protease mapping in 2DE gels / Zhenjun Zhao and Pamela J. Russell -- Two-dimensional difference gel electrophoresis / Gert Van den Bergh -- Protein expression profiling / Brian P. Bradley, Bose Kalampanayil, and Michael C. O'Neill -- C-terminal sequence analysis of 2DE-separated proteins / Bart Samyn, Kjell Sergeant, and Jozef Van Beeumen -- Shotgun protein analysis by liquid chromatography-tandem mass spectrometry / Kazuishi Kubota, Toshiyuki Kosaka, and Kimihisa Ichikawa --De novo sequence analysis of N-terminal sulfonated peptides after in-gel guanidination / Kjell Sergeant, Jozef Van Beeumen, and Bart Samyn -- Tryptic digestion of in-gel proteins for mass spectrometry analysis / Mai-Loan Huynh, Pamela Russell, and Bradley Walsh -- Database interrogation algorithms for identification of proteins in proteomic separations / Patricia M. Palagi, Frédérique Lisacek, and Ron D. Appel -- Creating 2DE databases for the world wide web / Christine Hoogland, Khaled Mostaguir, and Ron D. Appel.
Summary: Annotation Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists.
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Includes bibliographical references and index.

Two-dimensional electrophoresis : an overview / Richard Smith -- Solubilization of proteins in 2DE : an outline / Thierry Rabilloud -- Selection of pH ranges in 2DE / Mireille Starita-Geribaldi -- Difficult proteins / Ben Herbert and Elizabeth Harry -- Organelle proteomics / Matthias Plöscher [and others] -- Applications of chemical tagging approaches in combination with 2DE and mass spectrometry / Alexander Leitner and Wolfgang Lindner -- Immunoblotting 2DE membranes / Brian McDonagh -- Troubleshooting image analysis in 2DE / Bettina Levänen and Åsa M. Wheelock -- Analysis of bacterial proteins by 2DE / Philip Cash and Evelyn Argo -- Proteomic analysis of Caenorhabditis elegans / Pan-Young Jeong [and others] -- Protein extraction for 2DE / Claus Zabel and Joachim Klose -- Analysis of proteins from marine molluscs / Suze Chora, Maria João Bebianno, and Michèle Roméo -- Preparation and analysis of plastid proteomes by 2DE / Anne von Zychlinski and Wilhelm Gruissem -- High resolution 2DE / Katrin Marcus [and others] -- Blue native-gel electrophoresis proteomics / Kelly Andringa, Adrienne King, and Shannon Bailey -- 2DE for proteome analysis of human metaphase chromosomes / Kiichi Fukui and Susumu Uchiyama -- Microsomal proteomics / Diana M. Wong and Khosrow Adeli -- Prefractionation using microscale solution IEF / Won-A Joo and David Speicher -- Diagonal electrophoresis for detection of protein disulphide bridges / Brian McDonagh -- High-resolution large-gel 2DE / Claus Zabel and Joachim Klose -- Silver staining of proteins in 2DE gels / Cécile Lelong [and others] -- Detection of 4-hydroxy-2-nonenal- and 3-nitrotyrosine-modified proteins using a proteomics approach / Rukhsana Sultana, Tanea Reed, and D. Allan Butterfield -- Proteomic detection of oxidized and reduced thiol proteins in cultured cells / Sarah L. Cuddihy [and others] -- Detection of ubiquitination in 2DE / Brian McDonagh -- Phosphoroproteome analysis by in-gel isoelectric focusing and tandem mass spectrometry / Sarka Beranova-Georgianni, Dominic M. Desiderio, and Francesco Giorgianni -- Detection of protein glutathionylation / Elisabetta Gianazza, Ivano Eberini, and Pietro Ghezzi -- Activity-based protein profiling of protein tyrosine phosphatases / Chad Walls, Bo Zhou, and Zhong-Yin Zhang -- Active protease mapping in 2DE gels / Zhenjun Zhao and Pamela J. Russell -- Two-dimensional difference gel electrophoresis / Gert Van den Bergh -- Protein expression profiling / Brian P. Bradley, Bose Kalampanayil, and Michael C. O'Neill -- C-terminal sequence analysis of 2DE-separated proteins / Bart Samyn, Kjell Sergeant, and Jozef Van Beeumen -- Shotgun protein analysis by liquid chromatography-tandem mass spectrometry / Kazuishi Kubota, Toshiyuki Kosaka, and Kimihisa Ichikawa --De novo sequence analysis of N-terminal sulfonated peptides after in-gel guanidination / Kjell Sergeant, Jozef Van Beeumen, and Bart Samyn -- Tryptic digestion of in-gel proteins for mass spectrometry analysis / Mai-Loan Huynh, Pamela Russell, and Bradley Walsh -- Database interrogation algorithms for identification of proteins in proteomic separations / Patricia M. Palagi, Frédérique Lisacek, and Ron D. Appel -- Creating 2DE databases for the world wide web / Christine Hoogland, Khaled Mostaguir, and Ron D. Appel.

Annotation Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists.

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