Nucleic acid and peptide aptamers : methods and protocols / edited by Günter Mayer.

Contributor(s): Mayer, Günter, 1972-
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 535.; Springer protocols (Series): Publisher: New York : Humana, ©2009Description: 1 online resource (xi, 408 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781597455572; 1597455571Subject(s): Oligonucleotides | Nucleotide sequence | Amino acid sequence | Aptamers, Nucleotide | Aptamers, Peptide | Amino acid sequence | Nucleotide sequence | Oligonucleotides | Animal Biochemistry | Human Anatomy & Physiology | Health & Biological Sciences | aptameren | aptamers | geneesmiddelen | drugs | geneeskunde | medicine | biologische technieken | biological techniques | protocollen | protocols | Nucleic AcidsGenre/Form: Electronic books. Additional physical formats: Print version:: Nucleic acid and peptide aptamers.DDC classification: 572.8/4 LOC classification: QP625.O47Online resources: Click here to access online
Contents:
Nucleic acid pool preparation and characterization / S.K. Piasecki, B. Hall and A.D. Ellington -- In vitro selection of ssDNA aptamers using biotinylated target proteins / G. Mayer and T. Hover -- Isolating aptamers using capillary electrophoresis-SELEX (CE-SELEX) / R.K. Mosing and M.T. Bowser -- In vitro selection of allosteric ribozymes / N. Piganeau -- Cell-specific aptamers for targeted therapies / L. Cerchia [and others] -- Aptamers targeting RNA molecules / M. Watrin [and others] -- Fluorescence correlation spectroscopy (FCS)-based characterisation of aptamer ligand interaction / A. Werner and U. Hahn -- Structural probing techniques on natural aptamers / C.A. Wakeman and W.C. Winkler -- Determining structures of RNA aptamers and riboswitches by X-ray crystallography / A.L. Edwards, A.D. Garst and R.T. Batey -- Locked nucleic acid aptamers / J. Barciszewski [and others] -- Screening of novel inhibitors of HIV-1 reverse transcriptase with a reporter ribozyme assay / S. Yamazaki and M. Famulok -- Aptamers as artificial gene regulation elements / B. Suess and J.E. Weigand -- Aptamers and biosensors / T.M. Gronewold -- Nanoparticles/Dip stick / Y. Lu, J. Liu and D. Mazumdar -- In vivo imaging of oligonucleotidic aptamers / B. Tavitian [and others] -- Selection of peptides interfering with protein-protein interaction / A. Gaida [and others] -- In vitro selection of protein and peptide libraries using mRNA display / T.T. Takahashi and R.W. Roberts -- Ligand-regulated peptide aptamers / R.A. Miller -- Isolation of peptide aptamers to target protein function / L. Lopez-Ochoa [and others] -- Microbodies / H.U. Schmoldt [and others] -- Peptide aptamers for small molecule drug discovery / C. Bardou [and others] -- Synthesis and application of peptides as drug carriers / R. Rennert, I. Neundorf and A.G. Beck-Sickinger.
Summary: After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In Nucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.
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Includes bibliographical references and index.

Nucleic acid pool preparation and characterization / S.K. Piasecki, B. Hall and A.D. Ellington -- In vitro selection of ssDNA aptamers using biotinylated target proteins / G. Mayer and T. Hover -- Isolating aptamers using capillary electrophoresis-SELEX (CE-SELEX) / R.K. Mosing and M.T. Bowser -- In vitro selection of allosteric ribozymes / N. Piganeau -- Cell-specific aptamers for targeted therapies / L. Cerchia [and others] -- Aptamers targeting RNA molecules / M. Watrin [and others] -- Fluorescence correlation spectroscopy (FCS)-based characterisation of aptamer ligand interaction / A. Werner and U. Hahn -- Structural probing techniques on natural aptamers / C.A. Wakeman and W.C. Winkler -- Determining structures of RNA aptamers and riboswitches by X-ray crystallography / A.L. Edwards, A.D. Garst and R.T. Batey -- Locked nucleic acid aptamers / J. Barciszewski [and others] -- Screening of novel inhibitors of HIV-1 reverse transcriptase with a reporter ribozyme assay / S. Yamazaki and M. Famulok -- Aptamers as artificial gene regulation elements / B. Suess and J.E. Weigand -- Aptamers and biosensors / T.M. Gronewold -- Nanoparticles/Dip stick / Y. Lu, J. Liu and D. Mazumdar -- In vivo imaging of oligonucleotidic aptamers / B. Tavitian [and others] -- Selection of peptides interfering with protein-protein interaction / A. Gaida [and others] -- In vitro selection of protein and peptide libraries using mRNA display / T.T. Takahashi and R.W. Roberts -- Ligand-regulated peptide aptamers / R.A. Miller -- Isolation of peptide aptamers to target protein function / L. Lopez-Ochoa [and others] -- Microbodies / H.U. Schmoldt [and others] -- Peptide aptamers for small molecule drug discovery / C. Bardou [and others] -- Synthesis and application of peptides as drug carriers / R. Rennert, I. Neundorf and A.G. Beck-Sickinger.

Print version record.

After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In Nucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.

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