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Type 2 diabetes : methods and protocols / edited by Claire Stocker.

Contributor(s): Stocker, ClaireMaterial type: TextTextSeries: Springer protocols (Series) | Methods in molecular biology (Clifton, N.J.) ; v. 560.Publication details: New York : Humana Press, ©2009. Description: 1 online resource (x, 278 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781597454483; 1597454486Subject(s): Non-insulin-dependent diabetes -- Laboratory manuals | Non-insulin-dependent diabetes -- Susceptibility -- Research | Non-insulin-dependent diabetes -- Research | Physiology | Diabetes Mellitus, Type 2 | Gene Expression Profiling | Laboratory Manuals | Pancreas | Insulin | Islets of Langerhans | Mice | Digestive System | Proinsulin | Genetic Techniques | Endocrine Glands | Diabetes Mellitus | Publication Formats | Biological Science Disciplines | Murinae | Natural Science Disciplines | Anatomy | Publication Characteristics | Pancreatic Hormones | Endocrine System | Endocrine System Diseases | Glucose Metabolism Disorders | Investigative Techniques | Muridae | Metabolic Diseases | Peptide Hormones | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Rodentia | Disciplines and Occupations | Disease | Hormones | Peptides | Nutritional and Metabolic Diseases | Mammals | Hormones, Hormone Substitutes, and Hormone Antagonists | Vertebrates | Amino Acids, Peptides, and Proteins | Chemicals and Drugs | Chordata | Animals | Eukaryota | Organisms | Non-insulin-dependent diabetes | Diabète de type 2 -- Cahiers de laboratoire | Diabetes mellitus | Typ 2 | Diabète -- Manuels de laboratoire | diabetes mellitus | laboratoriummethoden | laboratory methods | protocollen | protocols | Cellular Biology | Human Pathology | Celbiologie | Humane pathologieGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Type 2 diabetes.DDC classification: 616.4620072 LOC classification: RC662.18NLM classification: WK 25Online resources: Click here to access online
Contents:
1. Selecting the "right" mouse model for metabolic syndrome and type 2 diabetes research / Edward H. Leiter -- 2. Nutritional models of type 2 diabetes mellitus / Beverly Sara Muhlhausler -- 3. The isolation and purification of rodent pancreatic islets of Langerhans / Jacqueline F.O ̓Dowd -- 4. The measurement of insulin secretion from isolated rodent islets of Langerhans / Anna L. Nolan and Jacqueline F.O ̓Dowd -- 5. The incubation and monitoring of cell viability in primary rat islets of Langerhans and pancreatic B-cell lines / Noel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell -- 6. In vitro culture of isolated islets of Langerhans : analysis of function / Anna L. Nolan -- 7. Single cell RT-PRC identification of genes expressed by human islet endocrine cells / Dany Muller, Peter M. Jones, and Shanta J. Persaud -- 8. Laser capture microdissection of human pancreatic B-cells and RNA preparation for gene expression profiling / Lorella Marselli [and others] -- 9. In vitro transdifferentiation of human hepatoma cells into pancreatic-like cells / Wan-chun Li -- 10. The measurement of GLUT4 translocation in 3T3-L1 adipocytes / Nicky Konstantopoulos and Juan Carlos Molero-Navajas -- 11. Pancreatic remodeling : beta cell apoptosis, proliferation and neogenesis and the measurement of beta cell mass and of individual beta cell size / Eduard Montanya and Noélia Téllez -- 12. Morphology of pancreatic islets : a time course of pre-diabetes in Zucker fatty rats / Petra Augstein and Eckhard Salzsieder -- 13. Fluorescent Immunohistochemistry and in situ hybridization analysis of pancreas / Xiuli Wang, Shundi Ge, and Gay M. Crooks -- 14. The measurement of insulin secretion using pancreas perfusion in the rodent / Edward T. Wargent -- 15. Hyperinsulinemic-euglycemic clamp to assess insulin sensitivity in vivo / Jason K. Kim -- 16. Gene expression analysis in diabetes research / Peter White and Klaus H. Kaestner -- 17. Gene expression mining in type 2 diabetes research / Donald R. Dunbar.
Action note: digitized 2010 committed to preserveSummary: Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.
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Includes bibliographical references and index.

1. Selecting the "right" mouse model for metabolic syndrome and type 2 diabetes research / Edward H. Leiter -- 2. Nutritional models of type 2 diabetes mellitus / Beverly Sara Muhlhausler -- 3. The isolation and purification of rodent pancreatic islets of Langerhans / Jacqueline F.O ̓Dowd -- 4. The measurement of insulin secretion from isolated rodent islets of Langerhans / Anna L. Nolan and Jacqueline F.O ̓Dowd -- 5. The incubation and monitoring of cell viability in primary rat islets of Langerhans and pancreatic B-cell lines / Noel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell -- 6. In vitro culture of isolated islets of Langerhans : analysis of function / Anna L. Nolan -- 7. Single cell RT-PRC identification of genes expressed by human islet endocrine cells / Dany Muller, Peter M. Jones, and Shanta J. Persaud -- 8. Laser capture microdissection of human pancreatic B-cells and RNA preparation for gene expression profiling / Lorella Marselli [and others] -- 9. In vitro transdifferentiation of human hepatoma cells into pancreatic-like cells / Wan-chun Li -- 10. The measurement of GLUT4 translocation in 3T3-L1 adipocytes / Nicky Konstantopoulos and Juan Carlos Molero-Navajas -- 11. Pancreatic remodeling : beta cell apoptosis, proliferation and neogenesis and the measurement of beta cell mass and of individual beta cell size / Eduard Montanya and Noélia Téllez -- 12. Morphology of pancreatic islets : a time course of pre-diabetes in Zucker fatty rats / Petra Augstein and Eckhard Salzsieder -- 13. Fluorescent Immunohistochemistry and in situ hybridization analysis of pancreas / Xiuli Wang, Shundi Ge, and Gay M. Crooks -- 14. The measurement of insulin secretion using pancreas perfusion in the rodent / Edward T. Wargent -- 15. Hyperinsulinemic-euglycemic clamp to assess insulin sensitivity in vivo / Jason K. Kim -- 16. Gene expression analysis in diabetes research / Peter White and Klaus H. Kaestner -- 17. Gene expression mining in type 2 diabetes research / Donald R. Dunbar.

Print version record.

Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.

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