Reverse chemical genetics : methods and protocols / edited by Hisashi Koga.

Contributor(s): Koga, Hisashi
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 577.Publisher: New York, NY : Humana Press, ©2009Description: 1 online resource (xi, 272 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781607612322; 1607612321Subject(s): Biochemical genetics | Methods | Molecular Biology | Laboratory Manuals | Combinatorial Chemistry Techniques | Genetic Techniques | Investigative Techniques | Publication Formats | Biochemistry | Technology, Pharmaceutical | Genetics | Biological Science Disciplines | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Biology | Publication Characteristics | Chemistry | Natural Science Disciplines | Disciplines and Occupations | Biochemical genetics | Biochemie | Genetik | Labortechnik | Methode | genetica | genetics | moleculaire genetica | molecular genetics | dna cloning | recombinant eiwitten | recombinant proteins | eiwitexpressieanalyse | proteomics | Molecular Genetics | Moleculaire geneticaGenre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Reverse Chemical Genetics.DDC classification: 572.8 LOC classification: QH430Online resources: Click here to access online
Contents:
ORFeome cloning / Osamu Ohara -- Systematic cloning of an ORFeome using the gateway system / Akihisa Matsuyama and Minoru Yoshida -- High-throughput construction of ORF clones for production of the recombinant proteins / Hisashi Yamakawa -- Computational overview of GPCR gene universe to support reverse chemical genomics study / Makiko Suwa and Yukiteru Ono -- High-performance gene expression module analysis tool and its application to chemical toxicity data / Wataru Fujibuchi [and others] -- Human protein reference database and human proteinpedia as discovery tools for systems biology / T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey -- High-throughput production of the recombinant proteins expressed in Escherichia coli utilizing cDNA resources / Kiyo Shimada and Hisashi Koga -- An insect cell-free system for recombinant protein expresion using cDNA resources / Takashi Syuzuki [and others] -- Recombinant protein production by a kaiko-baculovirus system / Hidekazu Nagaya -- Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins / Kei Yamaguchi [and others] -- Application of 2D-DIGE in cancer proteomics toward personalized medicine / Tadashi Kondo and Setsuo Hirohashi -- Fully automated two-dimensional electrophoresis system for high-throughput protein analysis / Atsunori Hiratsuka and Kenji Yokoyama -- Large-scale whole mount in situ hybridization of mouse embryos / Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara -- Identification of the specific binding proteins of bioactive small coumpound using affinity resins / Akito Tanaka -- Protein microarrays : effective tools for the study of inflammatory diseases / Xiaobo Yu [and others] -- Preparation of highly sensitive protein array using reactive polymer / Toshifumi Shiroya [and others] -- High-throughput SPR biosensor / Motoki Kyo [and others] -- Drug discovery through functional screening in the Drosophila heart / Takeshi Akasaka and Karen Ocorr -- Permeable cell assay : a method for high-throughput measurement of cellular ATP synthetic activity / Kiyotaka Y. Hara -- Novel methodology for immobilization of biomolecules on the surface of a photoresponsible polymer containing azobenzene moiety / Osamu Watanabe.
Action note: digitized 2010 committed to preserveSummary: Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.
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Includes bibliographical references and index.

ORFeome cloning / Osamu Ohara -- Systematic cloning of an ORFeome using the gateway system / Akihisa Matsuyama and Minoru Yoshida -- High-throughput construction of ORF clones for production of the recombinant proteins / Hisashi Yamakawa -- Computational overview of GPCR gene universe to support reverse chemical genomics study / Makiko Suwa and Yukiteru Ono -- High-performance gene expression module analysis tool and its application to chemical toxicity data / Wataru Fujibuchi [and others] -- Human protein reference database and human proteinpedia as discovery tools for systems biology / T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey -- High-throughput production of the recombinant proteins expressed in Escherichia coli utilizing cDNA resources / Kiyo Shimada and Hisashi Koga -- An insect cell-free system for recombinant protein expresion using cDNA resources / Takashi Syuzuki [and others] -- Recombinant protein production by a kaiko-baculovirus system / Hidekazu Nagaya -- Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins / Kei Yamaguchi [and others] -- Application of 2D-DIGE in cancer proteomics toward personalized medicine / Tadashi Kondo and Setsuo Hirohashi -- Fully automated two-dimensional electrophoresis system for high-throughput protein analysis / Atsunori Hiratsuka and Kenji Yokoyama -- Large-scale whole mount in situ hybridization of mouse embryos / Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara -- Identification of the specific binding proteins of bioactive small coumpound using affinity resins / Akito Tanaka -- Protein microarrays : effective tools for the study of inflammatory diseases / Xiaobo Yu [and others] -- Preparation of highly sensitive protein array using reactive polymer / Toshifumi Shiroya [and others] -- High-throughput SPR biosensor / Motoki Kyo [and others] -- Drug discovery through functional screening in the Drosophila heart / Takeshi Akasaka and Karen Ocorr -- Permeable cell assay : a method for high-throughput measurement of cellular ATP synthetic activity / Kiyotaka Y. Hara -- Novel methodology for immobilization of biomolecules on the surface of a photoresponsible polymer containing azobenzene moiety / Osamu Watanabe.

Print version record.

Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.

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Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. MiAaHDL

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