Single nucleotide polymorphisms : methods and protocols / edited by Anton A. Komar.
Contributor(s): Komar, Anton AMaterial type: TextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 578.Publisher: New York, NY : Humana Press, ©2009Edition: 2nd edDescription: 1 online resource (xiv, 464 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781603274111; 1603274111Subject(s): Single nucleotide polymorphisms -- Laboratory manuals | Chromosome polymorphism -- Laboratory manuals | Human genetics -- Variation -- Laboratory manuals | Genetic markers -- Laboratory manuals | Polymorphism, Single Nucleotide | Genotype | Laboratory Manuals | Genetic Markers | Genetics | DNA Mutational Analysis | Methods | Biomarkers | Sequence Analysis, DNA | Biology | Investigative Techniques | Polymorphism, Genetic | Publication Formats | Genetic Phenomena | Phenotype | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Sequence Analysis | Biological Science Disciplines | Genetic Variation | Phenomena and Processes | Biological Factors | Publication Characteristics | Genetic Techniques | Chemicals and Drugs | Natural Science Disciplines | Disciplines and Occupations | Single nucleotide polymorphisms | Chromosome polymorphism | Genetic markers | Human genetics -- Variation | genetische merkers | genetic markers | genkartering | gene mapping | biologische technieken | biological techniques | polymerase chain reaction | single nucleotide polymorphism | genotyping | protocollen | protocols | moleculaire merkers | molecular markers | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Single nucleotide polymorphisms.DDC classification: 611.0181663 LOC classification: QH447.6Online resources: Click here to access online
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Includes bibliographical references and index.
SNPs : impact on gene function and phenotype / B.S. Shastry -- Silent (synonymous) SNPs : should we care about them? / R. Hunt [and others] -- SNP databases / C. Phillips -- Mining SNPs from DNA sequence data : computational approaches to SNP discovery and analysis / J. van Oeveren and A. Janssen -- Next-generation sequencing methods : impact of sequencing accuracy on SNP discovery / E.Y. Chan -- Scanning probe and nanopore DNA sequencing : core techniques and possibilities / J. Lund and B.A. Parviz -- Pyrosequencing for SNP genotyping / J.L. Royo and J.J. Galan -- Single nucleotide polymorphism screening with denaturing gradient gel electrophoresis / L.A. Knapp -- Temporal temperature gradient electrophoresis for detection of single nucleotide polymorphisms / B.M. Jones and L.A. Knapp -- Zn(II)-cyclen polyacrylamide gel electrophoresis for SNP detection / E. Kinoshita-Kikuta, E. Kinoshita and T. Koike -- Phosphate-affinity polyacrylamide gel electrophoresis for SNP genotyping / E. Kinoshita, E. Kinoshita-Kikuta and T. Koike -- Estimation of SNP allele frequencies by SSCP analysis of pooled DNA / T. Tahira [and others] -- Phenylethynylpyrene excimer forming hybridization probes for fluorescence SNP detection / I.A. Prokhorenko [and others] -- The chemical cleavage of mismatch for the detection of mutations in long DNA fragments / T. Tabone, G. Sallmann and R.G. Cotton -- Mismatch oxidation assay : detection of DNA mutations using a standard UV/Vis microplate reader / T. Tabone, G. Sallmann and R.G. Cotton -- High-throughput methods for SNP genotyping / C. Ding and S. Jin -- High-throughput SNP genotyping : combining tag SNPs and molecular beacons / L.B. Barreiro, R. Henriques and M.M. Mhlanga -- SNP genotyping by the 5'-nuclease reaction : advances in high-throughput genotyping with nonmodel organisms / J.E. Seeb [and others] -- The TaqMan method for SNP genotyping / G.Q. Shen, K.G. Abdullah and Q.K. Wang -- Qualitative and quantitative genotyping using single base primer extension coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MassARRAY) / P. Oeth [and others] -- SNP detection using trityl mass tags / K.R. Birikh [and others] -- Putting the Invader assay to work : laboratory application and data management / Y. Zhang, E. Smith and M. Olivier -- SNP genotyping using multiplex single base primer extension assays / D. Podini and P.M. Vallone -- High-throughput SNP detection based on PCR amplification on magnetic nanoparticles using dual-color hybridization / N. He, S. Li and H. Liu -- Restriction enzyme analysis of PCR products / M. Ota [and others] -- Allele-specific PCR in SNP genotyping / M. Gaudet [and others] -- Modified multiple primer extension method / T. Yanagawa and H. Koga -- Detection of SNP by the isothermal smart amplification method / A. Lezhava and Y. Hayashizaki.
Print version record.
In recent years, single nucleotide polymorphisms have received increased and special attention in a rapidly developing field of personalized medicine and drug treatment. Comprising more than eighty percent of all known polymorphisms, single nucleotide polymorphisms (SNPs) are primarily responsible for phenotypic differences between individuals, and have been suggested to affect the development of diseases in humans and the response to drug treatment and environmental stress. In Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition, expert researchers explore the latest advances in this area, highlighting the substantial progress that has been made in SNP genotyping, examining recent developments in high-throughput genotyping approaches, and exploring our new understanding of the impact of SNPs on gene function. Chapters address the impact of SNPs on phenotype, examine SNP databases, look at methods that have been applied for SNP bioinformatics discovery and analysis, and discuss advanced experimental approaches used for SNP detection. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Current and innovative, Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition is an essential guidebook for individual researchers as well as institutions and companies working in the field.