Yeast functional genomics and proteomics : methods and protocols / edited by Igor Stagljar.

Contributor(s): Stagljar, Igor
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 548.; Springer protocols (Series): Publisher: Dordrecht ; New York : Humana Press, ©2009Description: 1 online resource (xiii, 300 pages, 4 unnumbered pages of plates) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781597455404; 1597455407Subject(s): Saccharomyces cerevisiae -- Genetics | Molecular genetics -- Technique | Saccharomyces cerevisiae -- genetics | Genetic Techniques | Genomics -- methods | Laboratory Techniques and Procedures | Proteomics -- methods | Molecular genetics -- Technique | Saccharomyces cerevisiae -- Genetics | Techniques génétiques | Génomique | Techniques et procédures de laboratoire | Protéomique | Hefeartige Pilze | Genanalyse | Untersuchungsmethode | Proteomanalyse | Saccharomyces cerevisiae | Génétique moléculaire | gisten | yeasts | saccharomyces cerevisiae | methodologie | methodology | functionele genomica | functional genomics | protocollen | protocols | eiwitexpressieanalyse | proteomics | Fungi | SchimmelsGenre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Yeast functional genomics and proteomics.DDC classification: 579.56313 LOC classification: QH470.S23Online resources: Click here to access online
Contents:
Comparative genome hybridization on tiling microarrays to detect aneuploidies in yeast / Barry Dion and Grant W. Brown -- Identification of transcription factor targets by phenotypic activation and microarray expression profiling in yeast / Gordon Chua -- SGAM: an array-based approach for high-resolution genetic mapping in Saccharomyces cerevisiae / Michael Costanzo and Charles Boone -- Reporter-based synthetic genetic array analysis: a functional genomics approach for investigating the cell cycle in Saccharomyces cerevisiae / Holly E. Sassi [and others] -- The fidgety yeast: focus on high-resolution live yeast cell microscopy / Heimo Wolinski, Klaus Natter, and Sepp D. Kohlwein -- A genomic approach to yeast chronological aging / Christopher R. Burtner, Christopher J. Murakami, and Matt Kaeberlein -- Chemogenomic approaches to elucidation of gene function and genetic pathways / Sarah E. Pierce [and others] -- Identification of inhibitors of chromatin modifying enzymes using the yeast phenotypic screens / Benjamin Newcomb and Antonio Bedalov -- Exploiting yeast genetics to inform therapeutic strategies for Huntington's disease / Flaviano Giorgini and Paul J. Muchowski -- Global proteomic analysis of Saccharomyces cerevisiae identifies molecular pathways of histone modifications / Jessica Jackson and Ali Shilatifard -- Systematic characterization of the protein interaction network and protein complexes in Saccharomyces cerevisiae using tandem affinity purification and mass spectrometry / Mohan Babu [and others] -- Protein microarrays / Joseph Fasolo and Michael Snyder -- Analysis of protein-protein interactions using array-based yeast two-hybrid screens / Seesandra V. Rajagopala and Peter Uetz -- Analysis of membrane protein complexes using the split-ubiquitin membrane yeast two-hybrid (MYTH) system / Saranya Kittanakom [and others] -- Computational analysis of the yeast proteome: understanding and exploiting functional specificity in genomic data / Curtis Huttenhower [and others].
Abstract: Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. The chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
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Includes bibliographical references and index.

Comparative genome hybridization on tiling microarrays to detect aneuploidies in yeast / Barry Dion and Grant W. Brown -- Identification of transcription factor targets by phenotypic activation and microarray expression profiling in yeast / Gordon Chua -- SGAM: an array-based approach for high-resolution genetic mapping in Saccharomyces cerevisiae / Michael Costanzo and Charles Boone -- Reporter-based synthetic genetic array analysis: a functional genomics approach for investigating the cell cycle in Saccharomyces cerevisiae / Holly E. Sassi [and others] -- The fidgety yeast: focus on high-resolution live yeast cell microscopy / Heimo Wolinski, Klaus Natter, and Sepp D. Kohlwein -- A genomic approach to yeast chronological aging / Christopher R. Burtner, Christopher J. Murakami, and Matt Kaeberlein -- Chemogenomic approaches to elucidation of gene function and genetic pathways / Sarah E. Pierce [and others] -- Identification of inhibitors of chromatin modifying enzymes using the yeast phenotypic screens / Benjamin Newcomb and Antonio Bedalov -- Exploiting yeast genetics to inform therapeutic strategies for Huntington's disease / Flaviano Giorgini and Paul J. Muchowski -- Global proteomic analysis of Saccharomyces cerevisiae identifies molecular pathways of histone modifications / Jessica Jackson and Ali Shilatifard -- Systematic characterization of the protein interaction network and protein complexes in Saccharomyces cerevisiae using tandem affinity purification and mass spectrometry / Mohan Babu [and others] -- Protein microarrays / Joseph Fasolo and Michael Snyder -- Analysis of protein-protein interactions using array-based yeast two-hybrid screens / Seesandra V. Rajagopala and Peter Uetz -- Analysis of membrane protein complexes using the split-ubiquitin membrane yeast two-hybrid (MYTH) system / Saranya Kittanakom [and others] -- Computational analysis of the yeast proteome: understanding and exploiting functional specificity in genomic data / Curtis Huttenhower [and others].

Print version record.

Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. The chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

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