Histology protocols / edited by Tim D. Hewitson, Ian A. Darby.

Contributor(s): Hewitson, Tim D | Darby, Ian A
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 611.Publisher: New York, N.Y. : Humana Press, ©2010Description: 1 online resource (x, 229 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781603273459; 160327345XSubject(s): Histology -- Laboratory manuals | Microscopy | Histocytological Preparation Techniques | Methods | Cytological Techniques | Optics and Photonics | Investigative Techniques | Histological Techniques | Diagnostic Imaging | Clinical Laboratory Techniques | Diagnostic Techniques and Procedures | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Physics | Natural Science Disciplines | Diagnosis | Disciplines and Occupations | Histology | Cytology | Anatomy | Biology | Human Anatomy & Physiology | Health & Biological Sciences | histologie | histology | histochemie | histochemistry | pathogenese | pathogenesis | kleuring | staining | methodologie | methodology | technieken | techniques | protocollen | protocols | Histology | Biological Techniques | Histologie | Biologische techniekenGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Histology protocols.DDC classification: 611/.01078 LOC classification: QM556Online resources: Click here to access online
Contents:
Tissue preparation for histochemistry : fixation, embedding, and antigen retrieval for light microscopy / Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker -- An optimized RNA extraction method from archival formalin-fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt -- Laser-capture microdissection and pressure catapulting for the analysis of gene expression in the renal glomerulus / Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly -- Immunofluorescence detection of the cytoskeleton and extracellular matrix in tissue and cultured cells / Josiane Smith-Clerc and Boris Hinz -- Double immunohistochemistry with horseradish peroxidase and alkaline phosphatase detection systems / Vincent Sarrazy and Alexis Desmoulière -- Retrogradely transported neuronal tracers combined with immunohistochemistry using free-floating brain sections / Emilio Badoer -- High-pressure freezing, chemical fixation, and freeze-substitution for immuno-electron microscopy / Christian Muhfeld -- Lectin histochemistry for light and electron microscopy / Su Ee Wong [and others] -- Duplex in situ hybridization in the study of gene co-regulation in the vertebrate brain / Raphael Pinaud and Jin K. Jeong -- Special stains for extracellular matrix / Andréa Monte-Alto-Costa and Luís Cristóvão Porto -- Active staining of mouse embryos for magnetic resonance microscopy / Alexandra Petiet and G. Allan Johnson -- Immunohistochemical detection of tumour hypoxia / Richard J. Young and Andreas Möller -- In situ localization of apoptosis using TUNEL / Tim D. Hewitson and Ian A. Darby -- Use of confocal microscopy for three-dimensional imaging of neurons in the spinal cord / Martin Stebbing [and others] -- High-resolution confocal imaging in tissue / Verena C. Wimmer and Andreas Möller -- Software-based stacking techniques to enhance depth of field and dynamic range in digital photomicrography / Jörg Piper -- Image analysis and quantitative morphology / Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-Santos, and Marcia Barbosa Aguila.
Summary: With much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In Histology Protocols, expert researchers in the field contribute detailed methods involving the preparation of tissue, providing an overview of fixation, embedding, and processing, along with the required techniques for the retrieval of RNA from histological sections, followed by sections on routine and specialist histological staining techniques, including immuno-, lectin, and hybridization histochemistry, histological staining, as well as specific methods for the in situ identification of hypoxia and apoptosis, and details on the advances in imaging and image analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histology Protocols will provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource.
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Includes bibliographical references and index.

Tissue preparation for histochemistry : fixation, embedding, and antigen retrieval for light microscopy / Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker -- An optimized RNA extraction method from archival formalin-fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt -- Laser-capture microdissection and pressure catapulting for the analysis of gene expression in the renal glomerulus / Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly -- Immunofluorescence detection of the cytoskeleton and extracellular matrix in tissue and cultured cells / Josiane Smith-Clerc and Boris Hinz -- Double immunohistochemistry with horseradish peroxidase and alkaline phosphatase detection systems / Vincent Sarrazy and Alexis Desmoulière -- Retrogradely transported neuronal tracers combined with immunohistochemistry using free-floating brain sections / Emilio Badoer -- High-pressure freezing, chemical fixation, and freeze-substitution for immuno-electron microscopy / Christian Muhfeld -- Lectin histochemistry for light and electron microscopy / Su Ee Wong [and others] -- Duplex in situ hybridization in the study of gene co-regulation in the vertebrate brain / Raphael Pinaud and Jin K. Jeong -- Special stains for extracellular matrix / Andréa Monte-Alto-Costa and Luís Cristóvão Porto -- Active staining of mouse embryos for magnetic resonance microscopy / Alexandra Petiet and G. Allan Johnson -- Immunohistochemical detection of tumour hypoxia / Richard J. Young and Andreas Möller -- In situ localization of apoptosis using TUNEL / Tim D. Hewitson and Ian A. Darby -- Use of confocal microscopy for three-dimensional imaging of neurons in the spinal cord / Martin Stebbing [and others] -- High-resolution confocal imaging in tissue / Verena C. Wimmer and Andreas Möller -- Software-based stacking techniques to enhance depth of field and dynamic range in digital photomicrography / Jörg Piper -- Image analysis and quantitative morphology / Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-Santos, and Marcia Barbosa Aguila.

Print version record.

With much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In Histology Protocols, expert researchers in the field contribute detailed methods involving the preparation of tissue, providing an overview of fixation, embedding, and processing, along with the required techniques for the retrieval of RNA from histological sections, followed by sections on routine and specialist histological staining techniques, including immuno-, lectin, and hybridization histochemistry, histological staining, as well as specific methods for the in situ identification of hypoxia and apoptosis, and details on the advances in imaging and image analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histology Protocols will provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource.

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