Calcium measurement methods / edited by Alexei Verkhratsky, Ole H. Petersen.

Contributor(s): Verkhratskiĭ, A. N. (Alekseĭ Nestorovich) | Petersen, O. H. (Ole Holger)
Material type: TextTextSeries: Neuromethods: 43.Publisher: New York, N.Y. : Humana Press, ©2010Description: 1 online resource (xii, 254 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781607614760; 1607614766Subject(s): Calcium -- Physiological effect | Neurophysiology | Cellular signal transduction | Calcium channels | Calcium Channels | Calcium Signaling | Neurophysiology | Signal Transduction | Calcium | Neurons | Physiology | Biochemical Phenomena | Nervous System | Ion Channels | Second Messenger Systems | Ion Transport | Blood Coagulation Factors | Metals, Alkaline Earth | Cells | Biological Science Disciplines | Biological Transport | Anatomy | Elements | Membrane Glycoproteins | Membrane Transport Proteins | Natural Science Disciplines | Biological Factors | Metals | Membrane Proteins | Cell Physiological Phenomena | Inorganic Chemicals | Metabolism | Chemicals and Drugs | Disciplines and Occupations | Carrier Proteins | Chemical Phenomena | Proteins | Phenomena and Processes | Amino Acids, Peptides, and Proteins | Neurosciences | Calcium channels | Calcium -- Physiological effect | Cellular signal transduction | Neurophysiology | Animal Biochemistry | Human Anatomy & Physiology | Health & Biological Sciences | neurochemie | neurochemistry | biomedische wetenschappen | biomedicine | neurowetenschap | neuroscience | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Calcium measurement methods.DDC classification: 612.8 LOC classification: QP535.C2Online resources: Click here to access online
Contents:
Principles of the CA2+ homeostatic/signalling system / A. Verkhratsky and O.H. Petersen -- CA2+ recordings: Hardware and software (from microscopes to cameras) / E.C. Toescu and J. Graham -- CA2+ imaging: Principles of analysis and enhancement / F. Mammano and M. Bortolozzi -- Bioluminescent CA2+ indicators / L. Fedrizzi and M. Brini -- Monitoring calcium levels with genetically encoded indicators / O. Garaschuk and O. Griesbeck -- Intracellular calcium-sensitive microelectrodes / R.C. Thomas -- CA2+ caging and uncaging / S.H. Kim and M.K. Park -- CA2+ imaging of intracellular organelles: Endoplasmic reticulum / R. Blum, O.H. Petersen and A. Verkhratsky -- CA2+ imaging of intracellular organelles: Mitochondria / L. Núñez [and others] -- CA2+ imaging of dendrites and spines / K. Holthoff -- In vivo CA2+ imaging of the living brain using multi-cell bolus loading technique / G. Eichhoff [and others] -- CA2+ imaging of glia / C. Lohr and J.W. Deitmer.
Summary: Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signalling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In Calcium Measurement Methods, a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field. Cutting-edge and concise, Calcium Measurement Methods serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.
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Title from PDF title page (SpringerLink, viewed Dec. 10, 2009).

Includes bibliographical references and index.

Principles of the CA2+ homeostatic/signalling system / A. Verkhratsky and O.H. Petersen -- CA2+ recordings: Hardware and software (from microscopes to cameras) / E.C. Toescu and J. Graham -- CA2+ imaging: Principles of analysis and enhancement / F. Mammano and M. Bortolozzi -- Bioluminescent CA2+ indicators / L. Fedrizzi and M. Brini -- Monitoring calcium levels with genetically encoded indicators / O. Garaschuk and O. Griesbeck -- Intracellular calcium-sensitive microelectrodes / R.C. Thomas -- CA2+ caging and uncaging / S.H. Kim and M.K. Park -- CA2+ imaging of intracellular organelles: Endoplasmic reticulum / R. Blum, O.H. Petersen and A. Verkhratsky -- CA2+ imaging of intracellular organelles: Mitochondria / L. Núñez [and others] -- CA2+ imaging of dendrites and spines / K. Holthoff -- In vivo CA2+ imaging of the living brain using multi-cell bolus loading technique / G. Eichhoff [and others] -- CA2+ imaging of glia / C. Lohr and J.W. Deitmer.

Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signalling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In Calcium Measurement Methods, a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field. Cutting-edge and concise, Calcium Measurement Methods serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.

English.

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