Neuroproteomics : methods and protocols / edited by Andrew K. Ottens and Kevin K.W. Wang.

Contributor(s): Ottens, Andrew K | Wang, Kevin K. W
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 566.; Springer protocols (Series): Publisher: New York, N.Y. : Humana Press, ©2009Description: 1 online resource (xi, 321 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781597455626; 1597455628Subject(s): Proteomics | Nervous system -- Diseases -- Genetic aspects | Proteomics -- methods | Nervous System Diseases -- metabolism | Nervous System Physiological Phenomena -- genetics | Proteome -- isolation & purification | Nervous system -- Diseases -- Genetic aspects | Proteomics | fysiologie | physiology | biomedische wetenschappen | biomedicine | celfysiologie | cell physiology | neurowetenschap | neuroscience | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Neuroproteomics.DDC classification: 612.015 LOC classification: QP551Online resources: Click here to access online
Contents:
The methodology of neuroproteomics / Andrew K. Ottens -- Modeling cerebral ischemia in neuroproteomics / Jitendra R. Dave [and others] -- Clinical and model research of neurotrauma / András Büki [and others] -- Neuroproteomic methods in spinal cord injury / Anshu Chen and Joe E. Springer -- Modeling substance abuse for applications in proteomics / Scott E. Hemby and Nilesh Tannu -- Protein aggregate characterization in models of neurodegenerative disease / Andrew T.N. Tebbenkamp and David R. Borchelt -- Sub-proteome processing : isolation of neuromelanin granules from the human brain / Florian Tribl -- Proteomic analysis of protein phosphorylation and ubiquitination in Alzheimer's disease / Stefani N. Thomas, Diane Cripps, and Austin J. Yang -- Proteomics identification of carbonylated and HNE-bound brain proteins in Alzheimer's disease / Rukhsana Sultana and D. Allan Butterfield -- Mass spectrometric identification of in vivo nitrotyrosine sites in the human pituitary tumor proteome / Xianquan Zhan and Dominic M. Desiderio -- Improved enrichment and proteomic analysis of brain proteins with signaling function by heparin chromatography / Kurt Krapfenbauer and Michael Fountoulakis -- Calmodulin-binding proteome in the brain / Zhiqun Zhang [and others] -- Separation of the neuroproteome by ion exchange chromatography / Brian F. Fuller and Andrew K. Ottens -- iTRAQ-based shotgun neuroproteomics / Tong Liu, Jun Hu, and Hong Li -- Methods in drug abuse neuroproteomics : methamphetamine psychoproteome / Firas H. Kobeissy [and others] -- Shotgun protein identification and quantification by mass spectrometry in neuroproteomics / Bingwen Lu [and others] -- Identification of glycoproteins in human cerebrospinal fluid / Hye Jin Hwan, Thomas Quinn, and Jing Zhang -- Mass spectrometric analysis of body fluids for biomarker discovery / David M. Good and Joshua J. Coon -- Traumatic brain injury biomarkers : from pipeline to diagnostic assay development / Monika W. Oli [and others] -- Translation of neurological biomarkers to clinically relevant platforms / Ronald L. Hayes [and others].
Action note: digitized 2010 committed to preserveSummary: With the development of polypeptide ionization processes for mass spectrometry and the rapid processing of large-scale datasets with bioinformatics, researchers now have the tools necessary to propel neuroproteomics research to new levels. In Neuroproteomics: Methods and Protocols, experts in the neurological and analytical sciences present experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease. Divided into four convenient sections, this collection covers CNS animal models used for neuroproteomics research, methods for separating and analyzing discrete subcomponents of the neuroproteome, large-scale approaches for CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. As a volume in the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neuroproteomics: Methods and Protocols includes the clear "how-to" information needed to critically assess what can be accomplished in the field and how to best perform and evaluate neuroproteomic experiments in one's own research.
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Includes bibliographical references and index.

The methodology of neuroproteomics / Andrew K. Ottens -- Modeling cerebral ischemia in neuroproteomics / Jitendra R. Dave [and others] -- Clinical and model research of neurotrauma / András Büki [and others] -- Neuroproteomic methods in spinal cord injury / Anshu Chen and Joe E. Springer -- Modeling substance abuse for applications in proteomics / Scott E. Hemby and Nilesh Tannu -- Protein aggregate characterization in models of neurodegenerative disease / Andrew T.N. Tebbenkamp and David R. Borchelt -- Sub-proteome processing : isolation of neuromelanin granules from the human brain / Florian Tribl -- Proteomic analysis of protein phosphorylation and ubiquitination in Alzheimer's disease / Stefani N. Thomas, Diane Cripps, and Austin J. Yang -- Proteomics identification of carbonylated and HNE-bound brain proteins in Alzheimer's disease / Rukhsana Sultana and D. Allan Butterfield -- Mass spectrometric identification of in vivo nitrotyrosine sites in the human pituitary tumor proteome / Xianquan Zhan and Dominic M. Desiderio -- Improved enrichment and proteomic analysis of brain proteins with signaling function by heparin chromatography / Kurt Krapfenbauer and Michael Fountoulakis -- Calmodulin-binding proteome in the brain / Zhiqun Zhang [and others] -- Separation of the neuroproteome by ion exchange chromatography / Brian F. Fuller and Andrew K. Ottens -- iTRAQ-based shotgun neuroproteomics / Tong Liu, Jun Hu, and Hong Li -- Methods in drug abuse neuroproteomics : methamphetamine psychoproteome / Firas H. Kobeissy [and others] -- Shotgun protein identification and quantification by mass spectrometry in neuroproteomics / Bingwen Lu [and others] -- Identification of glycoproteins in human cerebrospinal fluid / Hye Jin Hwan, Thomas Quinn, and Jing Zhang -- Mass spectrometric analysis of body fluids for biomarker discovery / David M. Good and Joshua J. Coon -- Traumatic brain injury biomarkers : from pipeline to diagnostic assay development / Monika W. Oli [and others] -- Translation of neurological biomarkers to clinically relevant platforms / Ronald L. Hayes [and others].

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With the development of polypeptide ionization processes for mass spectrometry and the rapid processing of large-scale datasets with bioinformatics, researchers now have the tools necessary to propel neuroproteomics research to new levels. In Neuroproteomics: Methods and Protocols, experts in the neurological and analytical sciences present experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease. Divided into four convenient sections, this collection covers CNS animal models used for neuroproteomics research, methods for separating and analyzing discrete subcomponents of the neuroproteome, large-scale approaches for CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. As a volume in the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neuroproteomics: Methods and Protocols includes the clear "how-to" information needed to critically assess what can be accomplished in the field and how to best perform and evaluate neuroproteomic experiments in one's own research.

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