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Patch-clamp methods and protocols / edited by Peter Molnar and James J. Hickman.

Contributor(s): Molnar, Peter, 1965- | Hickman, James JMaterial type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 403.Publication details: Totowa, N.J. : [Paisley] : Humana ; [Quantum, distributor], ©2007. Description: 1 online resource (xi, 321 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781603270908; 1603270906; 9781597455299; 1597455296Subject(s): Patch-clamp techniques (Electrophysiology) | Neurosciences | Patch-Clamp Techniques | Electrophysiology | Ion Channel Gating | Synaptic Transmission | Patch-clamp techniques (Electrophysiology) | Patch-clamp techniques (Electrophysiology)Genre/Form: Electronic books. Additional physical formats: Print version:: Patch-clamp methods and protocols.DDC classification: 572.437 LOC classification: QP341 | .P337 2007NLM classification: QY 95Other classification: Q424 Online resources: Click here to access online
Contents:
Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDA receptors using the whole-cell patch-clamp method / László Fodor and József Nagy -- Memantine as an example of a fast, voltage-dependent, open channel N-methyl-D-aspartate receptor blocker / Chris G. Parsons and Kate Gilling -- Methods for evaluation of positive allosteric modulators of glutamate AMPA receptors / Edward R. Siuda, Jennifer C. Quirk, and Eric S. Nisenbaum -- Automated voltage-clamp technique / Andrea Ghetti, António Guia, and Jia Xu -- Flip-the-tip : automated patch clamping based on glass electrodes / Michael Fejtl [and others] -- The roboocyte : automated electrophysiology based on Xenopus oocytes / Christine Leisgen, Mike Kuester, and Christoph Methfessel -- Infrared-guided laser stimulation as a tool for elucidating the synaptic site of expression of long-term synaptic plasticity / Gerhard Rammes [and others] -- Single-cell RT-PCR, a technique to decipher the electrical, anatomical, and genetic determinants of neuronal diversity / Maria Toledo-Rodriguez and Henry Markram -- Mechanosensitive ion channels investigated simultaneously by scanning probe microscopy and patch clamp / Matthias G. Langer -- Synaptic connectivity in engineered neuronal networks / Peter Molnar [and others] -- Modeling of action potential generation in NG108-15 cells / Peter Molnar and James J. Hickman -- Whole-cell voltage clamp on skeletal muscle fibers with the silicone-clamp technique / Sandrine Pouvreau [and others] -- Determination of channel properties at the unitary level in adult mammalian isolated cardiomyocytes / Romain Guinamard -- Electrophysiological properties of embryonic stem cells during differentiation into cardiomyocyte-like cell types / Antoni C.G. van Ginneken and Arnoud C. Fijnvandraat -- Hybrid neuronal network studies under dynamic clamp / Alan D. Dorval II [and others] -- Cardiac channelopathies studied with the dynamic action potential-clamp technique / Géza Berecki [and others] -- Principles of single-channel kinetic analysis / Feng Qin -- Use of Xenopus oocytes to measure ionic selectivity of pore-forming peptides and ion channels / Thierry Cens and Pierre Charnet -- Estimation of quantal parameters with multiple-probability fluctuation analysis / Chiara Saviane and Robin Angus Silver.
Summary: Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.
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Includes bibliographical references and index.

Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDA receptors using the whole-cell patch-clamp method / László Fodor and József Nagy -- Memantine as an example of a fast, voltage-dependent, open channel N-methyl-D-aspartate receptor blocker / Chris G. Parsons and Kate Gilling -- Methods for evaluation of positive allosteric modulators of glutamate AMPA receptors / Edward R. Siuda, Jennifer C. Quirk, and Eric S. Nisenbaum -- Automated voltage-clamp technique / Andrea Ghetti, António Guia, and Jia Xu -- Flip-the-tip : automated patch clamping based on glass electrodes / Michael Fejtl [and others] -- The roboocyte : automated electrophysiology based on Xenopus oocytes / Christine Leisgen, Mike Kuester, and Christoph Methfessel -- Infrared-guided laser stimulation as a tool for elucidating the synaptic site of expression of long-term synaptic plasticity / Gerhard Rammes [and others] -- Single-cell RT-PCR, a technique to decipher the electrical, anatomical, and genetic determinants of neuronal diversity / Maria Toledo-Rodriguez and Henry Markram -- Mechanosensitive ion channels investigated simultaneously by scanning probe microscopy and patch clamp / Matthias G. Langer -- Synaptic connectivity in engineered neuronal networks / Peter Molnar [and others] -- Modeling of action potential generation in NG108-15 cells / Peter Molnar and James J. Hickman -- Whole-cell voltage clamp on skeletal muscle fibers with the silicone-clamp technique / Sandrine Pouvreau [and others] -- Determination of channel properties at the unitary level in adult mammalian isolated cardiomyocytes / Romain Guinamard -- Electrophysiological properties of embryonic stem cells during differentiation into cardiomyocyte-like cell types / Antoni C.G. van Ginneken and Arnoud C. Fijnvandraat -- Hybrid neuronal network studies under dynamic clamp / Alan D. Dorval II [and others] -- Cardiac channelopathies studied with the dynamic action potential-clamp technique / Géza Berecki [and others] -- Principles of single-channel kinetic analysis / Feng Qin -- Use of Xenopus oocytes to measure ionic selectivity of pore-forming peptides and ion channels / Thierry Cens and Pierre Charnet -- Estimation of quantal parameters with multiple-probability fluctuation analysis / Chiara Saviane and Robin Angus Silver.

Print version record.

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.

English.

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Patch-Clamp Methods and Protocols (Methods in Molecular Biology). by Molnar, Peter. ©2007

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