Protein misfolding and cellular stress in disease and aging : concepts and protocols / edited by Peter Bross and Niels Gregersen.

Contributor(s): Bross, Peter | Gregersen, Niels
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 648.Publisher: Totowa, N.J. : London : Humana ; Springer [distributor], 2010Description: 1 online resource (xi, 330 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781607617563; 1607617560Subject(s): Protein folding | Proteins -- Pathophysiology | Pathology, Cellular | Biophysical Phenomena | Medicine | Anatomy | Publication Formats | Biological Science Disciplines | Biochemical Phenomena | Growth and Development | Amino Acids, Peptides, and Proteins | Physical Phenomena | Health Occupations | Publication Characteristics | Physiological Phenomena | Chemicals and Drugs | Chemical Phenomena | Natural Science Disciplines | Protein Folding | Laboratory Manuals | Proteins | Physiology | Aging | Pathology | Cells | Disciplines and Occupations | Phenomena and Processes | Pathology, Cellular | Protein folding | Human Anatomy & Physiology | Medicine | Health & Biological Sciences | Animal Biochemistry | Pathology | celbiologie | cellular biology | levenswetenschappen | life sciences | eiwitexpressieanalyse | proteomics | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Protein misfolding and cellular stress in disease and aging.DDC classification: 611.01815 LOC classification: QP551Online resources: Click here to access online
Contents:
Protein misfolding and cellular stress : an overview -- Protein aggregation diseases: toxicity of soluble prefibrillar aggregates and their clinical significance -- Consequences of stress in the secretary pathway : the ER stress response and its role in the metabolic syndrome -- What role does mitochondrial stress play in neurodegenerative diseases? -- Autophagy in disease -- Mitophagy and mitoptosis in disease processes -- Cellular stress and protein misfolding during aging -- Measuring consequences of protein misfolding and cellular stress using OMICS techniques -- Production of cells with targeted integration of gene variants of human ABC transporter for stable and regulated expression using the Flp recombinase system -- Stress response profiles in human fibroblasts exposed to heat shock or oxidative stress -- Examining ubiquitinated protein aggregates in tissue sections -- Determination of proteasomal activities -- Measurement of autophagy in cells and tissues -- Transcription factor sequestration by polyglutamine proteins -- Biological membranes as protein aggregation matrices and targets of amyloid toxicity -- Measurement of mitochondrial ROS production -- In vivo detection of oxidized proteins : a practical approach to tissue-derived mitochondria -- Measurement of oxidized/reduced glutathione ratio -- Determination of altered mitochondria ultrastructure by electron microscopy -- Assessing bad sub-cellular localization under conditions associated with prevention or promotion of mitochondrial permeability transition-dependent toxicity -- A cellular viability assay to monitor drug toxicity -- Rescue of misfolded proteins and stabilization by small molecules.
Summary: How and why certain proteins misfold and how this misfolding is linked to many disease processes has become a well-documented topic of study. Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols moves beyond the basics to emphasize the molecular effects of protein misfolding at a cellular level, to delineate the impacts and cellular reactions that play a role in pathogenetic mechanisms, and to pinpoint possible manipulations and treatment strategies that can counteract, modify, or delay the consequences of misfolding. The volume begins with several concepts and approaches developed in the recent past including a connection to the research field of aging, where protein misfolding diseases have been equated to premature aging processes, and the book's coverage continues with detailed descriptions of protocols for relevant experimental approaches. Written in the highly successful Methods in Molecular Biology series format, protocols chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols aims to aid researchers in the field, as well as medical professionals and molecular biologists, in shaping and performing research related to this intriguing and vital subject.
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Includes bibliographical references and index.

Protein misfolding and cellular stress : an overview -- Protein aggregation diseases: toxicity of soluble prefibrillar aggregates and their clinical significance -- Consequences of stress in the secretary pathway : the ER stress response and its role in the metabolic syndrome -- What role does mitochondrial stress play in neurodegenerative diseases? -- Autophagy in disease -- Mitophagy and mitoptosis in disease processes -- Cellular stress and protein misfolding during aging -- Measuring consequences of protein misfolding and cellular stress using OMICS techniques -- Production of cells with targeted integration of gene variants of human ABC transporter for stable and regulated expression using the Flp recombinase system -- Stress response profiles in human fibroblasts exposed to heat shock or oxidative stress -- Examining ubiquitinated protein aggregates in tissue sections -- Determination of proteasomal activities -- Measurement of autophagy in cells and tissues -- Transcription factor sequestration by polyglutamine proteins -- Biological membranes as protein aggregation matrices and targets of amyloid toxicity -- Measurement of mitochondrial ROS production -- In vivo detection of oxidized proteins : a practical approach to tissue-derived mitochondria -- Measurement of oxidized/reduced glutathione ratio -- Determination of altered mitochondria ultrastructure by electron microscopy -- Assessing bad sub-cellular localization under conditions associated with prevention or promotion of mitochondrial permeability transition-dependent toxicity -- A cellular viability assay to monitor drug toxicity -- Rescue of misfolded proteins and stabilization by small molecules.

Print version record.

How and why certain proteins misfold and how this misfolding is linked to many disease processes has become a well-documented topic of study. Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols moves beyond the basics to emphasize the molecular effects of protein misfolding at a cellular level, to delineate the impacts and cellular reactions that play a role in pathogenetic mechanisms, and to pinpoint possible manipulations and treatment strategies that can counteract, modify, or delay the consequences of misfolding. The volume begins with several concepts and approaches developed in the recent past including a connection to the research field of aging, where protein misfolding diseases have been equated to premature aging processes, and the book's coverage continues with detailed descriptions of protocols for relevant experimental approaches. Written in the highly successful Methods in Molecular Biology series format, protocols chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols aims to aid researchers in the field, as well as medical professionals and molecular biologists, in shaping and performing research related to this intriguing and vital subject.

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