SiRNA and miRNA gene silencing : from bench to bedside / edited by Mouldy Sioud.

Contributor(s): Sioud, Mouldy
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 487.; Springer protocols (Series): Publisher: New York, NY : London : Humana Press ; Springer [distributor], ©2009Description: 1 online resource (xiv, 472 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781603275477; 1603275479; 9781603275460; 1603275460Subject(s): Small interfering RNA | Gene silencing | RNA, Antisense | RNA, Small Untranslated | Epigenesis, Genetic | RNA | Antisense Elements (Genetics) | RNA, Untranslated | Gene Expression Regulation | RNA, Small Interfering | MicroRNAs | Gene Silencing | Nucleic Acids, Nucleotides, and Nucleosides | Genetic Phenomena | Nucleic Acids | Chemicals and Drugs | Phenomena and Processes | Gene silencing | Small interfering RNA | Petit ARN d'interférence -- usage thérapeutique | Inactivation d'un gène | Interférence ARN | RNS-Interferenz | genexpressie | gene expression | biomedische wetenschappen | biomedicine | humane genetica | human genetics | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. | Aufsatzsammlung. Additional physical formats: Print version:: SiRNA and miRNA gene silencing.DDC classification: 572.88 LOC classification: QP623.5.S63 | S57 2009Online resources: Click here to access online
Contents:
Methods for selecting effective siRNA sequences by using statistical and clustering techniques / Shigeru Takasaki -- Deciphering the code of innate immunity recognition of siRNAs / Mouldy Sioud -- Targeted delivery of anitsense oligonucleotides and siRNAs into mammalian cells / Mouldy Sioud -- Local and systemic delivery of siRNAs for oligonucleotide therapy / Fumitaka Takeshita [and others] -- Imaging of siRNA delivery and silencing / Anna Moore and Zdravka Medarova -- Recent advances in magnetofection and its potential to deliver siRNAs in vitro / Olga Mykhaylyk [and others] -- In vitro and in vivo gene silencing by TransKingdom RNAi (tkRNAi) / Shuanglin Xiang [and others] -- Bacterial delivery of siRNAs : a new approach to solid tumor therapy / De-Qi Xu [and others] -- The therapeutic potential of LNA-modified siRNAs : reduction of off-target effects by chemical modification of the siRNA sequence / Kees Fluiter, Olaf R.F. Mook, and Frank Baas -- pSM155 and pSM30 vectors for miRNA and shRNA expression / Junzhu Wu, Akua N. Bonsra, and Guangwei Du -- Targeting oncogenes with siRNAs / Olaf Heidenreich -- Targeting stromal-cancer cell interactions with siRNAs / Seyedhossein Aharinejad [and others] -- Therapeutic potential of siRNA-mediated targeting of urokinase plasminogen activator, its receptor, and matrix metalloproteinases / Christopher S. Gondi and Jasti S. Rao -- Silencing of HIF-1[alpha] by RNA interference in human glioma cells in vitro and in vivo / David L. Gillespie [and others] -- RNA interference-mediated validation of genes involved in telomere maintenance and evasion of apoptosis as cancer therapeutic targets / Marco Folini, Marzia Pennati, and Nadia Zaffaroni -- Treating respiratory viral diseases with chemically modified, second generaton intranasal siRNAs/ Sailen Barik -- Progress in the therapeutic applications of siRNAs against HIV-1 / Miguel Angel Martínez -- Protein components of the microRNA pathway and human diseases / Marjorie P. Perron and Patrick Provost -- Intron-mediated RNA interference and microRNA biogenesis / Shao-Yao Ying and Shi-Lung Lin -- Emergence of a complex relationship between HIV-1 and the microRNA pathway / Dominique L. Ouellet [and others] -- Synthetic microRNA targeting glioma-associated antigen-1 protein / Naotake Tsuda [and others] -- Therapeutic targeting of gene expression by siRNAs directed against BCR-ABL transcripts in a patient with Imatinib-resistant chronic myeloid leukemia / Michael Koldehoff and Ahmet H. Elmaagacli.
Summary: RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA's unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection. Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.
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Includes bibliographical references and index.

Methods for selecting effective siRNA sequences by using statistical and clustering techniques / Shigeru Takasaki -- Deciphering the code of innate immunity recognition of siRNAs / Mouldy Sioud -- Targeted delivery of anitsense oligonucleotides and siRNAs into mammalian cells / Mouldy Sioud -- Local and systemic delivery of siRNAs for oligonucleotide therapy / Fumitaka Takeshita [and others] -- Imaging of siRNA delivery and silencing / Anna Moore and Zdravka Medarova -- Recent advances in magnetofection and its potential to deliver siRNAs in vitro / Olga Mykhaylyk [and others] -- In vitro and in vivo gene silencing by TransKingdom RNAi (tkRNAi) / Shuanglin Xiang [and others] -- Bacterial delivery of siRNAs : a new approach to solid tumor therapy / De-Qi Xu [and others] -- The therapeutic potential of LNA-modified siRNAs : reduction of off-target effects by chemical modification of the siRNA sequence / Kees Fluiter, Olaf R.F. Mook, and Frank Baas -- pSM155 and pSM30 vectors for miRNA and shRNA expression / Junzhu Wu, Akua N. Bonsra, and Guangwei Du -- Targeting oncogenes with siRNAs / Olaf Heidenreich -- Targeting stromal-cancer cell interactions with siRNAs / Seyedhossein Aharinejad [and others] -- Therapeutic potential of siRNA-mediated targeting of urokinase plasminogen activator, its receptor, and matrix metalloproteinases / Christopher S. Gondi and Jasti S. Rao -- Silencing of HIF-1[alpha] by RNA interference in human glioma cells in vitro and in vivo / David L. Gillespie [and others] -- RNA interference-mediated validation of genes involved in telomere maintenance and evasion of apoptosis as cancer therapeutic targets / Marco Folini, Marzia Pennati, and Nadia Zaffaroni -- Treating respiratory viral diseases with chemically modified, second generaton intranasal siRNAs/ Sailen Barik -- Progress in the therapeutic applications of siRNAs against HIV-1 / Miguel Angel Martínez -- Protein components of the microRNA pathway and human diseases / Marjorie P. Perron and Patrick Provost -- Intron-mediated RNA interference and microRNA biogenesis / Shao-Yao Ying and Shi-Lung Lin -- Emergence of a complex relationship between HIV-1 and the microRNA pathway / Dominique L. Ouellet [and others] -- Synthetic microRNA targeting glioma-associated antigen-1 protein / Naotake Tsuda [and others] -- Therapeutic targeting of gene expression by siRNAs directed against BCR-ABL transcripts in a patient with Imatinib-resistant chronic myeloid leukemia / Michael Koldehoff and Ahmet H. Elmaagacli.

Print version record.

RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA's unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection. Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.

English.

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