Cell-free protein production : methods and protocols / edited by Yaeta Endo, Kazuyuki Takai, and Takuya Ueda.

Contributor(s): Endō, Yaeta, 1946- | Takai, Kazuyuki | Ueda, Takuya
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 607.Publisher: New York : Humana Press, ©2010Description: 1 online resource (xi, 261 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781603273312; 160327331XSubject(s): Proteins -- Synthesis | Proteomics | Genetic translation | Proteomics | Methods | Physiology | Protein Biosynthesis | Biotechnology | Cell-Free System | Laboratory Manuals | Investigative Techniques | Biological Science Disciplines | Publication Formats | Technology | Genomics | Subcellular Fractions | Biochemistry | Peptide Biosynthesis | Gene Expression | Biochemical Phenomena | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Computational Biology | Genetic Phenomena | Genetics | Metabolism | Cellular Structures | Natural Science Disciplines | Publication Characteristics | Technology, Industry, and Agriculture | Chemistry | Disciplines and Occupations | Technology, Industry, Agriculture | Biology | Chemical Phenomena | Cells | Phenomena and Processes | Anatomy | Genetic translation | Proteins -- Synthesis | Proteomics | eiwitten | proteins | geneeskunde | medicine | Proteins and Enzymes | Eiwitten en enzymenGenre/Form: Electronic books. Additional physical formats: Print version:: Cell-free protein production.DDC classification: 572.645 LOC classification: QP551 | .C4534 2010Online resources: Click here to access online
Contents:
1. Cell-Free Protein Preparation through Prokaryotic Transcription-Translation Methods / Takanori Kigawa -- 2. PURE Technology / Yoshihiro Shimizu and Takuya Ueda -- 3. The Cell-Free Protein Synthesis System from Wheat Germ / Kazuyuki Takai and Yaeta Endo -- 4. A Cell-Free Protein Synthesis System from Insect Cells / Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, and Eiji Ando -- 5. Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells / Satoshi Mikami, Tominari Kobayashi, and Hiroaki Imataka -- 6. Analysis of Protein Functions through a Bacterial Cell-Free Protein Expression System / Takanori Kigawa -- 7. Cell-Free Based Protein Microarray Technology Using Agarose/DNA Microplate / Tatsuya Sawasaki and Yaeta Endo -- 8. An Efficient Approach to the Production of Vaccines Against the Malaria Parasite / Takafumi Tsuboi, Satoru Takeo, Tatsuya Sawasaki, Motomi Torii, and Yaeta Endo -- 9. Protein Engineering Accelerated by Cell-Free Technology / Takuya Kanno and Yuzuru Tozawa -- 10. Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds / Takanori Kigawa -- 11. NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System / Toshiyuki Kohno -- 12. Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination / Shin-ichi Makino, Michael A. Goren, Brian G. Fox, and John L. Markley -- 13. Cell-Free Protein Synthesis for Structure Determination by X-Ray Crystallography / Miki Watanabe, Ken-ichi Miyazono, Masaru Tanokura, Tatsuya Sawasaki, Yaeta Endo, and Ichizo Kobayashi -- 14. Production of Multi-Subunit Complexes on Liposome through an E. coli Cell-Free Expression System / Yutetsu Kuruma, Toshiharu Suzuki, and Takuya Ueda -- 15. Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System / Hiroyuki Hori -- 16. Strategies for the Cell-Free Expression of Membrane Proteins / Sina Reckel, Solmaz Sobhanifar, Florian Durst, Frank Löhr, Vladimir A. Shirokov, Volker Dötsch, and Frank Bernhard -- 17. Production of Membrane Proteins through the Wheat-Germ Cell-Free Technology / Akira Nozawa, Hideaki Nanamiya, and Yuzuru Tozawa -- 18. Ribosome Display with the PURE Technology / Takashi Kanamori, Hiroyuki Ohashi, and Takuya Ueda -- 19. Incorporation of 3-azidotyrosine into Proteins through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification / Takashi Yokogawa, Satoshi Ohno, and Kazuya Nishikawa -- 20. Synthesis of Functional Proteins within Liposomes / Takeshi Sunami, Tomoaki Matsuura, Hiroaki Suzuki, and Tetsuya Yomo.
Summary: During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Free Protein Production: Methods and Protocols aims to help researchers continue the growth of the vital exploration of cell-free sciences and technologies in order to better understand the dynamic lives of cells.
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Includes bibliographical references and index.

1. Cell-Free Protein Preparation through Prokaryotic Transcription-Translation Methods / Takanori Kigawa -- 2. PURE Technology / Yoshihiro Shimizu and Takuya Ueda -- 3. The Cell-Free Protein Synthesis System from Wheat Germ / Kazuyuki Takai and Yaeta Endo -- 4. A Cell-Free Protein Synthesis System from Insect Cells / Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, and Eiji Ando -- 5. Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells / Satoshi Mikami, Tominari Kobayashi, and Hiroaki Imataka -- 6. Analysis of Protein Functions through a Bacterial Cell-Free Protein Expression System / Takanori Kigawa -- 7. Cell-Free Based Protein Microarray Technology Using Agarose/DNA Microplate / Tatsuya Sawasaki and Yaeta Endo -- 8. An Efficient Approach to the Production of Vaccines Against the Malaria Parasite / Takafumi Tsuboi, Satoru Takeo, Tatsuya Sawasaki, Motomi Torii, and Yaeta Endo -- 9. Protein Engineering Accelerated by Cell-Free Technology / Takuya Kanno and Yuzuru Tozawa -- 10. Cell-Free Protein Production System with the E. coli Crude Extract for Determination of Protein Folds / Takanori Kigawa -- 11. NMR Assignment Method for Amide Signals with Cell-Free Protein Synthesis System / Toshiyuki Kohno -- 12. Cell-Free Protein Synthesis Technology in NMR High-Throughput Structure Determination / Shin-ichi Makino, Michael A. Goren, Brian G. Fox, and John L. Markley -- 13. Cell-Free Protein Synthesis for Structure Determination by X-Ray Crystallography / Miki Watanabe, Ken-ichi Miyazono, Masaru Tanokura, Tatsuya Sawasaki, Yaeta Endo, and Ichizo Kobayashi -- 14. Production of Multi-Subunit Complexes on Liposome through an E. coli Cell-Free Expression System / Yutetsu Kuruma, Toshiharu Suzuki, and Takuya Ueda -- 15. Synthesis of a Hetero Subunit RNA Modification Enzyme by the Wheat Germ Cell-Free Translation System / Hiroyuki Hori -- 16. Strategies for the Cell-Free Expression of Membrane Proteins / Sina Reckel, Solmaz Sobhanifar, Florian Durst, Frank Löhr, Vladimir A. Shirokov, Volker Dötsch, and Frank Bernhard -- 17. Production of Membrane Proteins through the Wheat-Germ Cell-Free Technology / Akira Nozawa, Hideaki Nanamiya, and Yuzuru Tozawa -- 18. Ribosome Display with the PURE Technology / Takashi Kanamori, Hiroyuki Ohashi, and Takuya Ueda -- 19. Incorporation of 3-azidotyrosine into Proteins through Engineering Yeast Tyrosyl-tRNA Synthetase and Its Application to Site-Selective Protein Modification / Takashi Yokogawa, Satoshi Ohno, and Kazuya Nishikawa -- 20. Synthesis of Functional Proteins within Liposomes / Takeshi Sunami, Tomoaki Matsuura, Hiroaki Suzuki, and Tetsuya Yomo.

Print version record.

During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Free Protein Production: Methods and Protocols aims to help researchers continue the growth of the vital exploration of cell-free sciences and technologies in order to better understand the dynamic lives of cells.

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