Surface plasmon resonance : methods and protocols / edited by Nico J. de Mol, Marcel J.E. Fischer.
Contributor(s): Mol, Nico J. de. (Nico Jan de) | Fischer, Marcel J. EMaterial type: TextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 627.Publisher: New York : Humana Press, ©2010Description: 1 online resource (x, 286 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781607616702; 160761670XSubject(s): Surface plasmon resonance | Surface Plasmon Resonance -- methods | Surface plasmon resonance | Oberflächenplasmonresonanz | Biomolekül | Methode | Atomic Physics | Physics | Physical Sciences & Mathematics | Oberflächenplasmonresonanz | biochemie | biochemistry | membranen | membranes | levenswetenschappen | life sciences | Biology (General) | Biologie (algemeen)Genre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Surface plasmon resonance.DDC classification: 543.5 LOC classification: QC176.8.P55 | S87 2010Online resources: Click here to access online
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Includes bibliographical references and index.
1. Surface plasmon resonance : a general introduction / Nico J. de Mol and Marcel J.E. Fischer -- 2. The Role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensing / Schuck and Huaying Zhao -- 3. Amine coupling through EDC/NHS : a practical approach / Marcel J.E. Fischer -- 4. High-affinity immobilization of proteins using biotin- and GST-based coupling strategies / Stephanie Q. Hutsell, Randall J. Kimple, David P. Siderovski, Francis S. Willard, and Adam J. Kimple -- 5. A Capture coupling method for the covalent immobilization of hexahistidine tagged proteins for surface plasmon resonance / Adam J. Kimple, Robin E. Muller, David P. Siderovski, and Francis S. Willard -- 6. Affinity constants for small molecules from SPR competition experiments / Nico J. de Mol -- 7. Surface plasmon resonance signal enhancement for immunoassay of small molecules / John S. Mitchell and Yinqiu Wu -- 8. High-throughput kinase assay based on surface plasmon resonance / Hiroyuki Takeda, Naoki Goshima, and Nobuo Nomura -- 9. SPR biosensor as a tool for screening prion protein binders as potential Antiprion Leads / Beining Chen -- 10. Carbohydrate-lectin interactions assayed by SPR / Eric Duverger, Nathalie Lamerant-Fayel, Natacha Frison, and Michel Monsigny -- 11. DNA sensors based on mixed self-assembled DNA/alkanethiol films / Sara Peeters and Tim Stakenborg -- 12. Preparation of lipid membrane surfaces for molecular interaction studies by surface plasmon resonance biosensors / Mojca Podlesnik Beseničar and Gregor Anderluh -- 13. Capture of intact liposomes on biacore sensor chips for protein-membrane interaction studies / Vesna Hodnik and Gregor Anderluh -- 14. Surface plasmon resonance spectroscopy for studying the membrane binding of antimicrobial peptides / Kristopher Hall and Marie-Isabel Aguilar -- 15. Surface plasmon resonance spectroscopy in determination of the interactions between amyloid [beta] proteins (A[beta]) and lipid membranes / Xu Hou, David H. Small, and Marie-Isabel Aguilar -- 16. Incorporation of a transmembrane protein into a supported 3D-matrix of liposomes for SPR studies / Annette Granéli -- 17. Application of surface plasmon resonance spectroscopy to study G-protein coupled receptor signaling / Konstantin E. Komolov and Karl-Wilhelm Koch -- 18. Integration of SPR biosensors with mass spectrometry (SPR-MS) / Dobrin Nedelkov -- 19. SPR/MS : recovery from sensorchips for protein identification by MALDI-TOF mass spectrometry / Jonas Borch and Peter Roepstorff.
Print version record.
While commercial instruments have expanded the usage and the related literature has increased, the quality of surface plasmon resonance (SPR) research has been hindered by a lack of knowledge of the processes that influence the SPR signal. In Surface Plasmon Resonance: Methods and Protocols, experts in the field present a wide variety of applications involving commercially available SPR instruments. The heart of the SPR technique is to construct a dedicated surface for an assay. Protocols describe such surfaces for many needs, including the study of membrane bound proteins. Beyond the protocols-based chapters, the volume also highlights the backgrounds of vital issues in the use of SPR, including processes occurring within the hydrogel environment of sensors and on lipid membrane surfaces as well as the analysis of kinetic information. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Surface Plasmon Resonance: Methods and Protocols fills a need for well-described, hands-on SPR experimental protocols and promises to inspire the adaptation of these techniques to fit the needs of labs around the world.