Molecular and cell biology methods for fungi / edited by Amir Sharon.
Contributor(s): Sharon, AmirMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 638.; Springer protocols (Series): Publisher: New York : Humana, ©2010Description: 1 online resource (xi, 321 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781607616115; 1607616114; 1607616106; 9781607616108Subject(s): Fungal molecular biology -- Laboratory manuals | Molecular Biology | Fungi | Eukaryota | Biochemistry | Genetics | Chemistry | Biological Science Disciplines | Biology | Organisms | Natural Science Disciplines | Disciplines and Occupations | Fungal molecular biology | Pilze | Molekularbiologie | Labortechnik | Cytologie | schimmels | fungi | moleculaire biologie | molecular biology | celbiologie | cellular biology | biologische technieken | biological techniques | laboratoriummethoden | laboratory methods | protocollen | protocols | genexpressieanalyse | genomics | Fungi | Biological Techniques | Schimmels | Biologische techniekenGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Molecular and cell biology methods for fungi.DDC classification: 572.8295 LOC classification: QK604.2.M64 | M645 2010Online resources: Click here to access online
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Includes bibliographical references and index.
Protoplast transformation of filamentous fungi / B. Gillian Turgeon [and others] -- Electroporation and Agrobacterium-mediated spore transformation / Anna Minz and Amir Sharon -- High-throughput construction of gene deletion cassettes for generation of Neurospora crassa knockout strains / Patrick D. Collopy [and others] -- Development of impala based transposon systems for gene tagging in filamentous fungi / Marie Dufresne and Marie-Josée Daboussi -- DelsGate : a robust and rapid method for gene deletion / María D. García-Pedrajas [and others] -- Gene silencing for functional analysis : assessing RNAi as a tool for manipulation of gene expression / Carmit Ziv and Oded Yarden -- Analysis of fungal gene expression by real time quantitative PCR / Shahar Ish-Shalom and Amnon Lichter -- Identification of differentially expressed fungal gene in planta by suppression subraction hybridization / Benjamin A. Horwitz and Sophie Lev -- Quantification of fungal infection of leaves with digital images and scion image software / Paul H. Goodwin and Tom Hsiang -- Expression profiling of fungal genes during arbuscular mycorrhiza symbiosis establishment using direct fluorescent in situ RT-PCR / Pascale M.A. Seddas-Dozolme [and others] -- Application of laser microdissection to study plant-fungal pathogen interactions / John Fosu-Nyarko, Michael G.K. Jones, and Zhaohui Wang -- Multiplex gene expression analysis by TRAC in fungal cultures / Jari J. Rautio -- Amlification of fungal genomes using multiple displacement amplification / Simon J. Foster and Brendon J. Monahan -- Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa / Jinhu Guo [and others] -- Measuring protein kinase and sugar kinase activity in plant pathogenic Fusarium species / Burton H Bluhm and Xinhua Zhao -- A detailed protocol for chromatin immunoprecipitation in the yeast Saccharomyces cerevisiae / Melanie Grably and David Engelberg -- A method to visualize the actin and microtubule cytoskeleton by indirect immunofluorescence / Flora Banuett -- Fluorescence in situ hybridization for molecular cytogenetic analysis in filamentous fungi / Dai Tsuchiya and Masatoki Taga -- Live-cell imaging of microtubule dynamics in hyphae of Neurospora crassa / Maho Uchida, Rosa R. Mouriño-Pérez, and Robert W. Roberson -- Methods to detect apoptotic-like cell death in filamentous fungi / Camile P. Semighini and Steven D. Harris -- Evaluation of antifungal susceptibility using flow cytometry / Cidália Pina-Vaz and Acácio Gonçalves Rodrigues -- Preparation of fungi for ultrastructural investigations and immunogoldlabelling / Gerd Hause and Simone Jahn -- Split-EGFP screens for the detection and localisation of protein-protein interactions in living yeast cells / Emma Barnard and David J. Timson.
Print version record.
With the development of fungal transformation systems and the deciphering of an increasing number of fungal genomes, this diverse clade of heterotrophic eukaryotic organisms has proven to be ideal for molecular work and highly serviceable as model systems to study basic processes with results that are applicable to many organisms, including humans. In Molecular and Cell Biology Methods for Fungi, experts in the field provide an up-to-date set of practical protocols covering a range of frequently used methods used to study molecular and cellular aspects of fungal biology. The included classical protocols such as transformation systems and traditional protein analysis methods, which have been widely used for many years, alongside the most advanced techniques such as genome amplification, whole genome knockout methods, and sophisticated in vivo imaging techniques will prove to be easily adaptable and useful in a wide range of species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Molecular and Cell Biology Methods for Fungi seeks to provide cutting-edge techniques in order to aid both experienced fungal research laboratories as well as those that are interested in using fungi as hosts to study their favorite genes.