Peptidomics : methods and protocols / edited by Mikhail Soloviev.

Contributor(s): Soloviev, Mikhail, 1965-
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 615.Publisher: New York, NY : Humana Press, ©2010Description: 1 online resource (xiv, 395 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781607615354; 1607615355Subject(s): Peptides | Proteomics | Peptides -- analysis | Peptides -- genetics | Mass Spectrometry -- methods | Neuropeptides | Proteomics -- methods | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | Peptides | Proteomics | Proteomanalyse | Untersuchungsmethode | Biochemistry | Chemistry | Physical Sciences & Mathematics | analytische scheikunde | analytical chemistry | levenswetenschappen | life sciences | celbiologie | cellular biology | eiwitexpressieanalyse | proteomics | peptiden | peptides | bioinformatics | laboratoriummethoden | laboratory methods | protocollen | protocols | Proteins and Enzymes | Eiwitten en enzymenGenre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Peptidomics.DDC classification: 612.015756 LOC classification: QD431 | .P47 2010Online resources: Click here to access online
Contents:
1. Peptidomics: divide et impera / Mikhail Soloviev -- 2. Performing comparative peptidomics analyses of Salmonella from different growth conditions / Joshua N. Adkins [and others] -- 3. Approaches to identify endogenous peptides in the soil nematode Caenorhabditis elegans / Steven J. Husson [and others] -- 4. Mass spectrometric analysis of Molluscan neuropeptides / Ka Wan Li and August B. Smit -- 5. Monitoring neuropeptides in vivo via microdialysis and mass spectrometry / Heidi L. Behrens and Lingjun Li -- 6. Protocols for peptidomic analysis of spider venoms / Liang Songping -- 7. Purification and characterization of biologically active peptides from spider venoms / Alexander A. Vassilevski [and others] -- 8. MALDI-TOF mass spectrometry approaches to the characterisation of insect Neuropeptides / Robert J. Weaver and Neil Audsley -- 9. Direct MALDI-TOF mass spectrometric peptide profiling of neuroendocrine tissue of Drosophila / Christian Wegener, Susanne Neupert, and Reinhard Predel -- 10. Direct peptide profiling of brain tissue by MALDI-TOF mass spectrometry / Joachim Schachtner [and others] -- 11. Peptidomic analysis of single identified neurons / Susanne Neupert and Reinhard Predel -- 12. Identification and analysis of bioactive peptides in amphibian skin secretions / J. Michael Conlon and Jérôme Leprince -- 13. An efficient protocol for DNA amplification of multiple amphibian skin antimicrobial peptide cDNAs / Shawichi Iwamuro and Tetsuya Kobayashi -- 14. Combined peptidomics and genomics approach to the isolation of amphibian antimicrobial peptides / Ren Lai -- 15. Identification and relative quantification of neuropeptides from the endocrine tissues / Kurt Boonen [and others] -- 16. Peptidome analysis of mouse liver tissue by size exclusion chromatography prefractionation / Lianghai Hu, Mingliang Ye, and Hanfa Zou -- 17. Rat brain neuropeptidomics: tissue collection, protease inhibition, neuropeptide extraction, and mass spectrometric analysis / Robert M. Sturm, James A. Dowell, and Lingjun Li -- 18. Quantitative neuroproteomics of the synapse / Dinah Lee Ramos-Ortolaza -- 19. Peptidomics analysis of lymphoblastoid cell lines / Anne Fogli and Philippe Bulet -- 20. Peptidomics: identification of pathogenic and marker peptides / Yang Xiang -- 21. Peptidomic approaches to the identification and characterization of functional peptides in hydra / Toshio Takahashi and Toshitaka Fujisawa -- 22. Immunochemical methods for the peptidomic analysis of tachykinin peptides and their precursors / Nigel M. Page and Nicola J. Weston-Bell -- 23. Affinity peptidomics: peptide selection and affinity capture on hydrogels and microarrays / Fan Zhang [and others] -- 24. In situ biosynthesis of peptide arrays / Mingyue He and Oda Stoevesandt -- 25. Bioinformatic approaches to the identification of novel neuropeptide precursors / Elke Clynen [and others] -- 26. Bioinformatic identification of plant peptides / Kevin A. Lease and John C. Walker.
Summary: Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism. In Peptidomics: Methods and Protocols, expert researchers present a comprehensive range of analytical techniques for the analysis of the peptide contents of complex biological samples with an emphasis often on higher throughput techniques, suitable for the analysis of large numbers of peptides typically present in the peptidomes. Encompassing a number of species ranging from bacteria to man, the methods presented intensively cover topics such as organism handling, tissue and organ dissection, cellular and subcellular fractionation, peptide extraction, fractionation and purification, structural characterization, molecular cloning, and sequence analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Peptidomics: Methods and Protocols brings this ten year old field fully up-to-date in order to inspire novices and experts alike with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide contents of complex biological samples.
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Includes bibliographical references and index.

1. Peptidomics: divide et impera / Mikhail Soloviev -- 2. Performing comparative peptidomics analyses of Salmonella from different growth conditions / Joshua N. Adkins [and others] -- 3. Approaches to identify endogenous peptides in the soil nematode Caenorhabditis elegans / Steven J. Husson [and others] -- 4. Mass spectrometric analysis of Molluscan neuropeptides / Ka Wan Li and August B. Smit -- 5. Monitoring neuropeptides in vivo via microdialysis and mass spectrometry / Heidi L. Behrens and Lingjun Li -- 6. Protocols for peptidomic analysis of spider venoms / Liang Songping -- 7. Purification and characterization of biologically active peptides from spider venoms / Alexander A. Vassilevski [and others] -- 8. MALDI-TOF mass spectrometry approaches to the characterisation of insect Neuropeptides / Robert J. Weaver and Neil Audsley -- 9. Direct MALDI-TOF mass spectrometric peptide profiling of neuroendocrine tissue of Drosophila / Christian Wegener, Susanne Neupert, and Reinhard Predel -- 10. Direct peptide profiling of brain tissue by MALDI-TOF mass spectrometry / Joachim Schachtner [and others] -- 11. Peptidomic analysis of single identified neurons / Susanne Neupert and Reinhard Predel -- 12. Identification and analysis of bioactive peptides in amphibian skin secretions / J. Michael Conlon and Jérôme Leprince -- 13. An efficient protocol for DNA amplification of multiple amphibian skin antimicrobial peptide cDNAs / Shawichi Iwamuro and Tetsuya Kobayashi -- 14. Combined peptidomics and genomics approach to the isolation of amphibian antimicrobial peptides / Ren Lai -- 15. Identification and relative quantification of neuropeptides from the endocrine tissues / Kurt Boonen [and others] -- 16. Peptidome analysis of mouse liver tissue by size exclusion chromatography prefractionation / Lianghai Hu, Mingliang Ye, and Hanfa Zou -- 17. Rat brain neuropeptidomics: tissue collection, protease inhibition, neuropeptide extraction, and mass spectrometric analysis / Robert M. Sturm, James A. Dowell, and Lingjun Li -- 18. Quantitative neuroproteomics of the synapse / Dinah Lee Ramos-Ortolaza -- 19. Peptidomics analysis of lymphoblastoid cell lines / Anne Fogli and Philippe Bulet -- 20. Peptidomics: identification of pathogenic and marker peptides / Yang Xiang -- 21. Peptidomic approaches to the identification and characterization of functional peptides in hydra / Toshio Takahashi and Toshitaka Fujisawa -- 22. Immunochemical methods for the peptidomic analysis of tachykinin peptides and their precursors / Nigel M. Page and Nicola J. Weston-Bell -- 23. Affinity peptidomics: peptide selection and affinity capture on hydrogels and microarrays / Fan Zhang [and others] -- 24. In situ biosynthesis of peptide arrays / Mingyue He and Oda Stoevesandt -- 25. Bioinformatic approaches to the identification of novel neuropeptide precursors / Elke Clynen [and others] -- 26. Bioinformatic identification of plant peptides / Kevin A. Lease and John C. Walker.

Print version record.

Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism. In Peptidomics: Methods and Protocols, expert researchers present a comprehensive range of analytical techniques for the analysis of the peptide contents of complex biological samples with an emphasis often on higher throughput techniques, suitable for the analysis of large numbers of peptides typically present in the peptidomes. Encompassing a number of species ranging from bacteria to man, the methods presented intensively cover topics such as organism handling, tissue and organ dissection, cellular and subcellular fractionation, peptide extraction, fractionation and purification, structural characterization, molecular cloning, and sequence analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Peptidomics: Methods and Protocols brings this ten year old field fully up-to-date in order to inspire novices and experts alike with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide contents of complex biological samples.

English.

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