Mycobacteria protocols / edited by Tanya Parish and Amanda Claire Brown.

Contributor(s): Parish, Tanya | Brown, Amanda Claire
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): volume 465.Publisher: New York, NY : Humana Press, ©2008Edition: 2nd edDescription: 1 online resource (xix, 456 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781597452076; 1597452076Subject(s): Mycobacteria -- Laboratory manuals | Mycobacterium | Genetic Techniques | Molecular Biology -- methods | Cytological Techniques | Mycobacteria | Mycobactériacées | microbiologie | microbiology | bacteriologie | bacteriology | geneeskunde | medicine | microorganisms | bacteria | Microbiology (General) | Microbiologie (algemeen)Genre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Mycobacteria protocols.DDC classification: 579.374 LOC classification: QR82.M8 | M935 2008Online resources: Click here to access online
Contents:
Isolation of Mycobacterium species genomic DNA / John T. Belisle, Spencer B. Mahaffey, and Preston J. Hill -- Isolation of mycobacterial RNA / Tige R. Rustad [and others] -- Lipoglycans of Mycobacterium tuberculosis : isolation, purification, and characterization / Libin Shi, Jordi B. Torrelles, and Delphi Chatterjee -- Analyzing lipid metabolism : activation and [beta]-oxidation of fatty acids / Paul Robert Wheeler -- Whole genome analysis using microarrays / S.J. Waddell, J. Hinds, and P.D. Butcher -- Use of DNA microarrays to study global patterns of gene expression / Roberta Provvedi, Giorgio Palù, and Riccardo Manganelli -- Two-dimensional gel electrophoresis-based proteomics of mycobacteria / Jens Mattow [and others] -- Transport assays and permeability in pathogenic mycobacteria / Marie-Antoinette Lanéelle and Mamadou Daffé -- Continuous culture of mycobacteria / Joanna Bacon and Kim A. Hatch -- Measuring minimum inhibitory concentrations in mycobacteria / Frederick A. Sirgel, Ian J.F. Wiid, and Paul D. van Helden -- Rapid screening of inhibitors of Mycobacterium tuberculosis growth using tetrazolium salts / Anita G. Amin [and others] -- Electroporation of mycobacteria / Renan Goude and Tanya Parish -- Ins and outs of mycobacterial plasmids / Farahnaz Movahedzadeh and Wilbert Bitter -- The use of temperature-sensitive plasmids in mycobacteria / Damien Portevin, Wladimir Malaga, and Christophe Guilhot -- Heterologous expression of genes in mycobacteria / James A. Triccas and Anthony A. Ryan -- Inducible expression systems for mycobacteria / Christopher M. Sassetti -- Assaying promoter activity using LacZ and GFP as reporters / Paul Carroll and Jade James -- Construction of unmarked deletion mutants in mycobacteria / Houhui Song, Frank Wolschendorf, and Michael Niederweis -- Construction of targeted mycobacterial mutants by homologous recombination / Sharon L. Kendall and Rosangela Frita -- Phage transposon mutagenesis / M. Sloan Siegrist and Eric J. Rubin -- Gene essentiality testing in Mycobacterium smegmatis using specialized transduction / Apoorva Bhatt and William R. Jacobs, Jr. -- Gene switching and essentiality testing / Amanda Claire Brown -- Insertion element IS6110-based restriction fragment length polymorphism genotyping of Mycobacterium tuberculosis / Robin M. Warren, Paul D. van Helden, and Nicolaas C. Gey van Pittius -- Typing Mycobacterium tuberculosis using variable number tandem repeat analysis / T.J. Brown, V.N. Nikolayevskyy, and F.A. Drobniewski -- Molecular detection of drug-resistant Mycobacterium tuberculosis with a scanning-frame oligonucleotide microarray / Dmitriy V. Volokhov [and others] -- MycoDB : an online database for comparative genomics of the mycobacteria and related organisms / Roy R. Chaudhuri -- Environmental amoebae and mycobacterial pathogenesis / Melanie Harriff and Luiz E. Bermudez.
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Includes bibliographical references and index.

Isolation of Mycobacterium species genomic DNA / John T. Belisle, Spencer B. Mahaffey, and Preston J. Hill -- Isolation of mycobacterial RNA / Tige R. Rustad [and others] -- Lipoglycans of Mycobacterium tuberculosis : isolation, purification, and characterization / Libin Shi, Jordi B. Torrelles, and Delphi Chatterjee -- Analyzing lipid metabolism : activation and [beta]-oxidation of fatty acids / Paul Robert Wheeler -- Whole genome analysis using microarrays / S.J. Waddell, J. Hinds, and P.D. Butcher -- Use of DNA microarrays to study global patterns of gene expression / Roberta Provvedi, Giorgio Palù, and Riccardo Manganelli -- Two-dimensional gel electrophoresis-based proteomics of mycobacteria / Jens Mattow [and others] -- Transport assays and permeability in pathogenic mycobacteria / Marie-Antoinette Lanéelle and Mamadou Daffé -- Continuous culture of mycobacteria / Joanna Bacon and Kim A. Hatch -- Measuring minimum inhibitory concentrations in mycobacteria / Frederick A. Sirgel, Ian J.F. Wiid, and Paul D. van Helden -- Rapid screening of inhibitors of Mycobacterium tuberculosis growth using tetrazolium salts / Anita G. Amin [and others] -- Electroporation of mycobacteria / Renan Goude and Tanya Parish -- Ins and outs of mycobacterial plasmids / Farahnaz Movahedzadeh and Wilbert Bitter -- The use of temperature-sensitive plasmids in mycobacteria / Damien Portevin, Wladimir Malaga, and Christophe Guilhot -- Heterologous expression of genes in mycobacteria / James A. Triccas and Anthony A. Ryan -- Inducible expression systems for mycobacteria / Christopher M. Sassetti -- Assaying promoter activity using LacZ and GFP as reporters / Paul Carroll and Jade James -- Construction of unmarked deletion mutants in mycobacteria / Houhui Song, Frank Wolschendorf, and Michael Niederweis -- Construction of targeted mycobacterial mutants by homologous recombination / Sharon L. Kendall and Rosangela Frita -- Phage transposon mutagenesis / M. Sloan Siegrist and Eric J. Rubin -- Gene essentiality testing in Mycobacterium smegmatis using specialized transduction / Apoorva Bhatt and William R. Jacobs, Jr. -- Gene switching and essentiality testing / Amanda Claire Brown -- Insertion element IS6110-based restriction fragment length polymorphism genotyping of Mycobacterium tuberculosis / Robin M. Warren, Paul D. van Helden, and Nicolaas C. Gey van Pittius -- Typing Mycobacterium tuberculosis using variable number tandem repeat analysis / T.J. Brown, V.N. Nikolayevskyy, and F.A. Drobniewski -- Molecular detection of drug-resistant Mycobacterium tuberculosis with a scanning-frame oligonucleotide microarray / Dmitriy V. Volokhov [and others] -- MycoDB : an online database for comparative genomics of the mycobacteria and related organisms / Roy R. Chaudhuri -- Environmental amoebae and mycobacterial pathogenesis / Melanie Harriff and Luiz E. Bermudez.

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