DNA replication : methods and protocols / edited by Sonya Vengrova and Jacob Z. Dalgaard.

Contributor(s): Vengrova, Sonya | Dalgaard, Jacob Z
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): 521.; Springer protocols (Series): Publisher: [New York?] : [London] : Humana ; [Springer, distributor], ©2009Description: 1 online resource (xiii, 692 pages, 6 unnumbered pages of plates) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781603278157; 160327815XSubject(s): DNA replication -- Research -- Methodology | DNA Replication | ADN -- Réplication -- Recherche -- Méthodologie | Animal genetics | Biochemical engineering | Genetic engineering | Life sciences | Replikation | Methode | Genetics | Animal Biochemistry | Biology | Human Anatomy & Physiology | Health & Biological Sciences | ADN | dna replication | engineering | biochemie | biochemistry | genetische modificatie | genetic engineering | genexpressieanalyse | genomics | diergenetica | animal genetics | Molecular Genetics | Moleculaire geneticaGenre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: DNA replication.DDC classification: 572.8645 LOC classification: QH506 | .M45 v.521 2009Online resources: Click here to access online
Contents:
DNA replication initiation / Stephen J. Aves -- DNA replication fork proteins / Ulrich Hübscher -- Random and site-specific replication termination / Jacob Z. Dalgaard [and others] -- Checkpoint regulation of DNA replication / Erik Boye, Henriette C. Skjølberg, and Beáta Grallert -- Introduction to molecular combing : genomics, DNA replication, and cancer / John Herrick and Aaron Bensimon -- Replication initiation point mapping : approach and implications / Sapna Das-Bradoo and Anja-Katrin Bielinsky -- Purification of restriction fragments containing replication intermediates from complex genomes for 2-D gel analysis / Larry D. Mesner, Pieter A. Dijkwel, and Joyce L. Hamlin -- Topological analysis of plasmid DNA replication intermediates using two-dimensional agarose gels / Olivier Hyrien -- Analysis of telomeric DNA replication using neutral-alkaline two-dimensional gel electrophoresis / Svetlana Makovets -- Chromatin immunoprecipitation of replication factors moving with the replication fork / Jordan B. Rapp [and others] -- Density transfer as a method to analyze the progression of DNA replication forks / José Antonio Tercero -- High-resolution mapping of points of site-specific replication stalling / Sonya Vengrova and Jacob Z. Dalgaard -- DNA replication in nucleus-free xenopus egg extracts / Ronald Lebofsky, Tatsuro Takahashi, and Johannes C. Walter -- ChIP-chip to analyze the binding of replication proteins to chromatin using oligonucleotide DNA microarrays / Christopher J. Viggiani, Jennifer G. Aparicio, and Oscar M. Aparicio -- Analyzing origin activation patterns by copy number change experiments / Miruthubashini Raveendranathan and Anja-Katrin Bielinsky -- Detection of replication origins using comparative genomics and recombinational ARS assay / Conrad A. Nieduszynski and Anne D. Donaldson -- Isolation of restriction fragments containing origins of replication from complex genomes / Larry D. Mesner and Joyce L. Hamlin -- Application of alkaline sucrose gradient centrifugation in the analysis of DNA replication after DNA damage / Sascha Raschke, Jun Guan, and George Iliakis -- Isolation of recombinant DNA elongation proteins / Barbara van Loon, Elena Ferrari, and Ulrich Hübscher -- In vitro assays for studying helicase activities / Jeong-Hoon Kim and Yeon-Soo Seo -- The use of 2-aminopurine fluorescence to study DNA polymerase function / Linda J. Reha-Krantz -- Single-molecule observation of prokaryotic DNA replication / Nathan A. Tanner and Antoine M. van Oijen -- Visualization of DNA replication sites in mammalian nuclei / Daniela S. Dimitrova -- Cell-cycle synchrony for analysis of S. pombe DNA replication / Douglas D. Luche and Susan L. Forsburg -- Measuring DNA content by flow cytometry in fission yeast / Sarah A. Sabatinos and Susan L. Forsburg -- Microscopy techniques to examine DNA replication in fission yeast / Marc D. Green, Sarah A. Sabatinos, and Susan L. Forsburg -- Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe / Stephen E. Kearsey and Juraj Gregan -- Assays used to study the DNA replication checkpoint in fission yeast / Eishi Noguchi [and others] -- Incorporation of thymidine analogs for studying replication kinetics in fission yeast / Nicholas Rhind -- The fast-halo assay for the assessment of DNA damage at the single-cell level / Piero Sestili -- Monitoring homologous recombination following replication fork perturbation in the fission yeast Schizosaccharomyces pombe / Fekret Osman and Matthew C. Whitby -- Computational methods to study kinetics of DNA replication / Scott Cheng-Hsing Yang, Michel G. Gauthier, and John Bechhoefer -- Use of DNA combing to study DNA replication in Xenopus and human cell-free systems / Kathrin Marheineke [and others] -- Electron microscopy methods for studying in vivo DNA replication intermediates / Massimo Lopes -- Determining the replication dynamics of specific gene loci by single-molecule analysis of replicated DNA / Agnieszka Demczuk and Paolo Norio -- Use of DNA combing for studying DNA replication in vivo in yeast and mammalian cells / Etienne Schwob [and others].
Summary: Since the discovery of DNA structure and throughout the ensuing "DNA era, " the field of DNA replication has expanded to cover a vast number of experimental systems. In DNA Replication: Methods and Protocols, expert researchers present a collection of techniques and approaches used to investigate DNA replication with an emphasis on the most recent technological developments. Beginning with several informative introductory review chapters, this extensive volume is organized for clarity while fully encouraging innovation by the mixing of methods to create new techniques. Written in the highly successful Methods in Molecular Biology series format, chapters contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Replication: Methods and Protocols provides an excellent tool for both established laboratories and individuals new to this exciting field of research.
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Includes bibliographical references and index.

DNA replication initiation / Stephen J. Aves -- DNA replication fork proteins / Ulrich Hübscher -- Random and site-specific replication termination / Jacob Z. Dalgaard [and others] -- Checkpoint regulation of DNA replication / Erik Boye, Henriette C. Skjølberg, and Beáta Grallert -- Introduction to molecular combing : genomics, DNA replication, and cancer / John Herrick and Aaron Bensimon -- Replication initiation point mapping : approach and implications / Sapna Das-Bradoo and Anja-Katrin Bielinsky -- Purification of restriction fragments containing replication intermediates from complex genomes for 2-D gel analysis / Larry D. Mesner, Pieter A. Dijkwel, and Joyce L. Hamlin -- Topological analysis of plasmid DNA replication intermediates using two-dimensional agarose gels / Olivier Hyrien -- Analysis of telomeric DNA replication using neutral-alkaline two-dimensional gel electrophoresis / Svetlana Makovets -- Chromatin immunoprecipitation of replication factors moving with the replication fork / Jordan B. Rapp [and others] -- Density transfer as a method to analyze the progression of DNA replication forks / José Antonio Tercero -- High-resolution mapping of points of site-specific replication stalling / Sonya Vengrova and Jacob Z. Dalgaard -- DNA replication in nucleus-free xenopus egg extracts / Ronald Lebofsky, Tatsuro Takahashi, and Johannes C. Walter -- ChIP-chip to analyze the binding of replication proteins to chromatin using oligonucleotide DNA microarrays / Christopher J. Viggiani, Jennifer G. Aparicio, and Oscar M. Aparicio -- Analyzing origin activation patterns by copy number change experiments / Miruthubashini Raveendranathan and Anja-Katrin Bielinsky -- Detection of replication origins using comparative genomics and recombinational ARS assay / Conrad A. Nieduszynski and Anne D. Donaldson -- Isolation of restriction fragments containing origins of replication from complex genomes / Larry D. Mesner and Joyce L. Hamlin -- Application of alkaline sucrose gradient centrifugation in the analysis of DNA replication after DNA damage / Sascha Raschke, Jun Guan, and George Iliakis -- Isolation of recombinant DNA elongation proteins / Barbara van Loon, Elena Ferrari, and Ulrich Hübscher -- In vitro assays for studying helicase activities / Jeong-Hoon Kim and Yeon-Soo Seo -- The use of 2-aminopurine fluorescence to study DNA polymerase function / Linda J. Reha-Krantz -- Single-molecule observation of prokaryotic DNA replication / Nathan A. Tanner and Antoine M. van Oijen -- Visualization of DNA replication sites in mammalian nuclei / Daniela S. Dimitrova -- Cell-cycle synchrony for analysis of S. pombe DNA replication / Douglas D. Luche and Susan L. Forsburg -- Measuring DNA content by flow cytometry in fission yeast / Sarah A. Sabatinos and Susan L. Forsburg -- Microscopy techniques to examine DNA replication in fission yeast / Marc D. Green, Sarah A. Sabatinos, and Susan L. Forsburg -- Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe / Stephen E. Kearsey and Juraj Gregan -- Assays used to study the DNA replication checkpoint in fission yeast / Eishi Noguchi [and others] -- Incorporation of thymidine analogs for studying replication kinetics in fission yeast / Nicholas Rhind -- The fast-halo assay for the assessment of DNA damage at the single-cell level / Piero Sestili -- Monitoring homologous recombination following replication fork perturbation in the fission yeast Schizosaccharomyces pombe / Fekret Osman and Matthew C. Whitby -- Computational methods to study kinetics of DNA replication / Scott Cheng-Hsing Yang, Michel G. Gauthier, and John Bechhoefer -- Use of DNA combing to study DNA replication in Xenopus and human cell-free systems / Kathrin Marheineke [and others] -- Electron microscopy methods for studying in vivo DNA replication intermediates / Massimo Lopes -- Determining the replication dynamics of specific gene loci by single-molecule analysis of replicated DNA / Agnieszka Demczuk and Paolo Norio -- Use of DNA combing for studying DNA replication in vivo in yeast and mammalian cells / Etienne Schwob [and others].

Print version record.

Since the discovery of DNA structure and throughout the ensuing "DNA era, " the field of DNA replication has expanded to cover a vast number of experimental systems. In DNA Replication: Methods and Protocols, expert researchers present a collection of techniques and approaches used to investigate DNA replication with an emphasis on the most recent technological developments. Beginning with several informative introductory review chapters, this extensive volume is organized for clarity while fully encouraging innovation by the mixing of methods to create new techniques. Written in the highly successful Methods in Molecular Biology series format, chapters contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Replication: Methods and Protocols provides an excellent tool for both established laboratories and individuals new to this exciting field of research.

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