Helicases : methods and protocols / edited by Mohamed M. Abdelhaleem.

Contributor(s): Abdelhaleem, Mohamed M
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 587.Publisher: New York, N.Y. : Humana Press, ©2010Description: 1 online resource (xi, 404 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781603273558; 1603273557Subject(s): DNA helicases | DNA Helicases | RNA Helicases | Laboratory Manuals | Publication Formats | Isomerases | Adenosine Triphosphatases | RNA Nucleotidyltransferases | Enzymes | Nucleotidyltransferases | Acid Anhydride Hydrolases | Publication Characteristics | Hydrolases | Enzymes and Coenzymes | Phosphotransferases | Transferases | Chemicals and Drugs | DNA helicases | Animal Biochemistry | Human Anatomy & Physiology | Health & Biological Sciences | enzymen | enzymes | laboratoriummethoden | laboratory methods | protocollen | protocols | moleculaire biologie | molecular biology | Proteins and Enzymes | Eiwitten en enzymenGenre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Helicases.DDC classification: 612.015 LOC classification: QP616.D54 | H45 2010Online resources: Click here to access online
Contents:
Helicases : an overview / Mohamed Abdelhaleem -- Fluorescent biosensors to investigate helicase activity / Martin R. Webb -- Single-molecule FRET analysis of helicase functions / Eli Rothenberg and Taekjip Ha -- Kinetics of motor protein translocation on single-stranded DNA / Christopher J. Fischer, Lake Wooten, Eric J. Tomko, and Timothy M. Lohman -- Experimental and computational analysis of DNA unwinding and polymerization kinetics / Manjula Pandey, Mikhail K. Levin, and Smita S. Patel -- Protein displacement by helicases / Laxmi Yeruva and Kevin D. Raney -- In vivo investigation of protein-protein interactions for helicases using tandem affinity purification / Matthew Jessulat, Terry Buist, Md Alamgir, Mohsen Hooshyar, Jianhua Xu, Hiroyuki Aoki, M. Clelia Ganoza, Gareth Butland, and Ashkan Golshani -- Mapping genomic targets of DNA helicases by chromatin immunoprecipitation in Saccharomyces cerevisiae / Jennifer Cobb and Haico van Attikum -- Methods to study how replication fork helicases unwind DNA / Daniel L. Kaplan and Irina Bruc -- Simple enzymatic assays for the in vitro motor activity of transcription termination factor rho from Escherichia coli / Marc Boudvillain, Céline Walmacq, Annie Schwartz, and Frédérique Jacquinot -- Single-molecule studies of RecBCD / Thomas T. Perkins and Hung-Wen Li -- Mutational analysis of bloom helicase / Xu Guang Xi -- In vivo analysis of Drosophila BLM helicase function during DNA double-strand gap repair / Mitch McVey -- Purification and characterization of RecQ helicases of plants / Daniela Kobbe, Manfred Focke, and Holger Puchta -- Fluorometric assay of Hepatitis C virus NS3 helicase activity / Mariusz Krawczyk, Anna Stankiewicz-Drogoń, Anne-Lise Haenni, and Anna Boguszewska-Chachulska -- A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities / David N. Frick, Olya Ginzburg, and Angela M.L. Lam -- Computer modeling of helicases using elastic network model / Wenjun Zheng -- Duplex unwinding with DEAD-box proteins / Eckhard Jankowsk and, Andrea Putnam -- Analysis of RNA helicase p68 (Ddx5) as a transcriptional regulator / Samantha M. Nicol and Frances V. Fuller-Pace -- A method to study the role of DDX3 RNA helicase in HIV-1 / Chia-Yen Chen, Venkat R.K. Yedavalli, and Kuan-Teh Jeang -- Molecular characterization of nuclear DNA helicase II (RNA helicase A) / Suisheng Zhang and Frank Grosse -- Regulation of inter- and intramolecular interaction of RNA, DNA, and proteins by MLE / Hyangyee Oh, Andrew M. Parrott, Yongkyu Park, and Chee-Gun Lee -- Biochemical characterization of human Upf1 helicase / Zhihong Cheng, Gaku Morisawa, and Haiwei Song -- Assays of the helicase, ATPase, and exoribonuclease activities of the yeast mitrochondrial degradosome / Michal Malecki, Piotr P. Stepien, and Pawel Golic -- Characterization of the helicase activity and anti-telomerase properties of yeast Pif1p in vitro / Jean-Baptiste Boulé and Virginia A. Zakian -- A method to confer salinity stress tolerance to plants by helicase overexpression / Narendra Tuteja -- A method to inhibit the growth of Plasmodium falciparum by double-stranded RNA-mediated gene silencing of helicases / Renu Tuteja.
Summary: In recent years, much information has been revealed concerning the essential role of helicases, the enzymes that utilize the energy derived from nucleoside triphosphate hydrolysis to unwind the double stranded helical structure of nucleic acids. In Helicases: Methods and Protocols, this topic is explored by leading international scientists who have contributed significantly to our current understanding of helicases and the ways in which they function. Chapters include methods for the production and purification of helicases from different species, as well as detailed studies of helicase activities, including NTP binding and hydrolysis, nucleic acid binding and unwinding, and translocation along nucleic acid substrates. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and innovative, Helicases: Methods and Protocols is the ultimate guide for researchers, providing the scientific community with comprehensive accounts of the most advanced methods used to study helicases today.
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Includes bibliographical references and index.

Helicases : an overview / Mohamed Abdelhaleem -- Fluorescent biosensors to investigate helicase activity / Martin R. Webb -- Single-molecule FRET analysis of helicase functions / Eli Rothenberg and Taekjip Ha -- Kinetics of motor protein translocation on single-stranded DNA / Christopher J. Fischer, Lake Wooten, Eric J. Tomko, and Timothy M. Lohman -- Experimental and computational analysis of DNA unwinding and polymerization kinetics / Manjula Pandey, Mikhail K. Levin, and Smita S. Patel -- Protein displacement by helicases / Laxmi Yeruva and Kevin D. Raney -- In vivo investigation of protein-protein interactions for helicases using tandem affinity purification / Matthew Jessulat, Terry Buist, Md Alamgir, Mohsen Hooshyar, Jianhua Xu, Hiroyuki Aoki, M. Clelia Ganoza, Gareth Butland, and Ashkan Golshani -- Mapping genomic targets of DNA helicases by chromatin immunoprecipitation in Saccharomyces cerevisiae / Jennifer Cobb and Haico van Attikum -- Methods to study how replication fork helicases unwind DNA / Daniel L. Kaplan and Irina Bruc -- Simple enzymatic assays for the in vitro motor activity of transcription termination factor rho from Escherichia coli / Marc Boudvillain, Céline Walmacq, Annie Schwartz, and Frédérique Jacquinot -- Single-molecule studies of RecBCD / Thomas T. Perkins and Hung-Wen Li -- Mutational analysis of bloom helicase / Xu Guang Xi -- In vivo analysis of Drosophila BLM helicase function during DNA double-strand gap repair / Mitch McVey -- Purification and characterization of RecQ helicases of plants / Daniela Kobbe, Manfred Focke, and Holger Puchta -- Fluorometric assay of Hepatitis C virus NS3 helicase activity / Mariusz Krawczyk, Anna Stankiewicz-Drogoń, Anne-Lise Haenni, and Anna Boguszewska-Chachulska -- A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities / David N. Frick, Olya Ginzburg, and Angela M.L. Lam -- Computer modeling of helicases using elastic network model / Wenjun Zheng -- Duplex unwinding with DEAD-box proteins / Eckhard Jankowsk and, Andrea Putnam -- Analysis of RNA helicase p68 (Ddx5) as a transcriptional regulator / Samantha M. Nicol and Frances V. Fuller-Pace -- A method to study the role of DDX3 RNA helicase in HIV-1 / Chia-Yen Chen, Venkat R.K. Yedavalli, and Kuan-Teh Jeang -- Molecular characterization of nuclear DNA helicase II (RNA helicase A) / Suisheng Zhang and Frank Grosse -- Regulation of inter- and intramolecular interaction of RNA, DNA, and proteins by MLE / Hyangyee Oh, Andrew M. Parrott, Yongkyu Park, and Chee-Gun Lee -- Biochemical characterization of human Upf1 helicase / Zhihong Cheng, Gaku Morisawa, and Haiwei Song -- Assays of the helicase, ATPase, and exoribonuclease activities of the yeast mitrochondrial degradosome / Michal Malecki, Piotr P. Stepien, and Pawel Golic -- Characterization of the helicase activity and anti-telomerase properties of yeast Pif1p in vitro / Jean-Baptiste Boulé and Virginia A. Zakian -- A method to confer salinity stress tolerance to plants by helicase overexpression / Narendra Tuteja -- A method to inhibit the growth of Plasmodium falciparum by double-stranded RNA-mediated gene silencing of helicases / Renu Tuteja.

Print version record.

In recent years, much information has been revealed concerning the essential role of helicases, the enzymes that utilize the energy derived from nucleoside triphosphate hydrolysis to unwind the double stranded helical structure of nucleic acids. In Helicases: Methods and Protocols, this topic is explored by leading international scientists who have contributed significantly to our current understanding of helicases and the ways in which they function. Chapters include methods for the production and purification of helicases from different species, as well as detailed studies of helicase activities, including NTP binding and hydrolysis, nucleic acid binding and unwinding, and translocation along nucleic acid substrates. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and innovative, Helicases: Methods and Protocols is the ultimate guide for researchers, providing the scientific community with comprehensive accounts of the most advanced methods used to study helicases today.

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