Heterologous expression of membrane proteins : methods and protocols / edited by Isabelle Mus-Veteau.

Contributor(s): Mus-Veteau, Isabelle
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 601.Publisher: New York : Humana Press, ©2010Description: 1 online resource (xi, 272 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781607613442; 1607613441Subject(s): Membrane proteins -- Analysis -- Methodology | Proteins | Amino Acids, Peptides, and Proteins | Chemicals and Drugs | Membrane Proteins | Heterologe Genexpression | Membranproteine | celbiologie | cellular biology | membranen | membranes | levenswetenschappen | life sciences | eiwitexpressieanalyse | proteomics | Biology (General) | Biologie (algemeen)Genre/Form: Electronic books. | Aufsatzsammlung. Additional physical formats: Print version:: Heterologous expression of membrane proteins.DDC classification: 572.694 LOC classification: QP552.M44Online resources: Click here to access online
Contents:
Heterologous expression of membrane proteins for structural analysis / Isabell Mus-Veteau.-- Production of membrane proteins in Escherichia coli and Lactococcus lactis / Eric R. Geertsma, Bert Poolman.-- Mammalian membrane receptors expression as inclusion bodies in Escherichia coli / Bernard Mouillac, Jean-Louis Baneres.-- Expression of membrane proteins at the Escherichia coli Membrane for structural studies / Manuela Zoonens, Bruno Miroux.-- Membrane protein expression in Lactococcus lactis / Annie Frelet-Barrand, Sylvain Boutigny, Edmund R.S. Kunji, Norbert Rolland.-- Heterologous expression of human membrane receptors in yeast Sacchraomyces cerevisiae / Olivier Joubert, Rony Nehme, Michel bidet, Isabelle Mus-Veteau.-- Mammalian membrane protein expression in baculovirus-infected insect cells / Celine Trometer, Pierre Falson.-- Expression of membrane proteins in Drosophila melanogaster S2 cells: production and analysis of a EGFP-fused G protein-coupled receptor as a model / Karl Brillet, Carlos A. Pereira, Renaud Wagner.-- Membrane protein epression in the eyes of transgenic flies / Valerie Panneels, Irmgard Sinning.-- Expression of mammalian membrane proteins in mammalian cells using semliki forest virus vectors / Kenneth Lundstrom.-- Membrane protein expression in cell-free systems / Birgit Schneider, Friederike Jnge, Vladimir A. Shirokov, Florian Durst, Daniel Schwarz, Volker Dotsch, Frank Bernhard.-- Practical considerations of membrane protein instability during purification and crystallization / Christopher G. Tate.-- Membrane protein solubilization / Katia Duquesne, James N. Sturgis.-- Amphipols and fluorinated surfactants: tow alternatives to detergents for studying ertrand Arnou, Marie Jidenko, Estelle Marchal, Marc Le Maire, Christine Jaxel.-- Amphipols and fluorinated surfactants: two alternatives to detergents for studying membrane proteins in vitro / Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc Le Maire, Christine Jaxel.-- Heterologous expression and affinity purification of eukaryotic membrane proteins in view of functional and structural studies: the example of the sarcoplasmic reticulum Ca2+-ATPase / Delphine Cardi, Cedric Montigny, Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc Le Maire, Christine Jaxel.
Summary: Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.
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Includes bibliographical references and index.

Heterologous expression of membrane proteins for structural analysis / Isabell Mus-Veteau.-- Production of membrane proteins in Escherichia coli and Lactococcus lactis / Eric R. Geertsma, Bert Poolman.-- Mammalian membrane receptors expression as inclusion bodies in Escherichia coli / Bernard Mouillac, Jean-Louis Baneres.-- Expression of membrane proteins at the Escherichia coli Membrane for structural studies / Manuela Zoonens, Bruno Miroux.-- Membrane protein expression in Lactococcus lactis / Annie Frelet-Barrand, Sylvain Boutigny, Edmund R.S. Kunji, Norbert Rolland.-- Heterologous expression of human membrane receptors in yeast Sacchraomyces cerevisiae / Olivier Joubert, Rony Nehme, Michel bidet, Isabelle Mus-Veteau.-- Mammalian membrane protein expression in baculovirus-infected insect cells / Celine Trometer, Pierre Falson.-- Expression of membrane proteins in Drosophila melanogaster S2 cells: production and analysis of a EGFP-fused G protein-coupled receptor as a model / Karl Brillet, Carlos A. Pereira, Renaud Wagner.-- Membrane protein epression in the eyes of transgenic flies / Valerie Panneels, Irmgard Sinning.-- Expression of mammalian membrane proteins in mammalian cells using semliki forest virus vectors / Kenneth Lundstrom.-- Membrane protein expression in cell-free systems / Birgit Schneider, Friederike Jnge, Vladimir A. Shirokov, Florian Durst, Daniel Schwarz, Volker Dotsch, Frank Bernhard.-- Practical considerations of membrane protein instability during purification and crystallization / Christopher G. Tate.-- Membrane protein solubilization / Katia Duquesne, James N. Sturgis.-- Amphipols and fluorinated surfactants: tow alternatives to detergents for studying ertrand Arnou, Marie Jidenko, Estelle Marchal, Marc Le Maire, Christine Jaxel.-- Amphipols and fluorinated surfactants: two alternatives to detergents for studying membrane proteins in vitro / Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc Le Maire, Christine Jaxel.-- Heterologous expression and affinity purification of eukaryotic membrane proteins in view of functional and structural studies: the example of the sarcoplasmic reticulum Ca2+-ATPase / Delphine Cardi, Cedric Montigny, Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc Le Maire, Christine Jaxel.

Print version record.

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.

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