RNA interference : from biology to clinical applications / edited by Wei-Ping Min, Thomas Ichim.

Contributor(s): Min, Wei-Ping | Ichim, Thomas, 1976-
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 623.; Springer protocols (Series): Publisher: New York : Humana Press, ©2010Description: 1 online resource (xvi, 450 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781607615880; 1607615886Subject(s): RNA editing | Gene silencing | RNA Interference | Gene Silencing | Epigenesis, Genetic | Gene Expression Regulation | Genetic Phenomena | Phenomena and Processes | Gene silencing | RNA editing | immunologie | immunology | geneeskunde | medicine | biomedische wetenschappen | biomedicine | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: RNA interference.DDC classification: 612.015 LOC classification: QH450.25 | .R63 2010Online resources: Click here to access online
Contents:
Endogenous antiviral mechanisms of RNA interference : a comparative biology perspective / Abubaker M.E. Sidahmed and Bruce Wilkie -- Monitoring innate immune recruitment by siRNAs in mammalian cells / Michael P. Gantier and Bryan R.G. Williams -- Current knowledge of microRNAs and noncoding RNAs in virus-infected cells / Dominique L. Ouellet and Patrick Provost -- Allele-specific silencing by RNA interference / Hirohiko Hohjoh -- Computational siRNA design considering alternative splicing / Young J. Kim -- Bioinformatic approaches to siRNA selection and optimization / Pirkko Muhonen and Harry Holthofer -- Optimized gene silencing by co-expression of mulitple shRNAs in a single vector / Yasuhito Ishigaki, Akihiro Nagao, and Tsukasa Matsunaga -- Strategies in designing multigene expression units to downregulate HIV-1 / Jane Zhang and John J. Rossi -- Designing optimal siRNA based on target site accessibility / Ivo L. Hofacker and Hakim Tafer -- Chemical synthesis of 2'-O-alkylated siRNAs / Joachim W. Engels [and others] -- siRNA specific delivery system for targeting dendritic cells / Xiufen Zheng [and others] -- Hydrodynamic delivery protocols / Piotr G. Rychahou and B. Mark Evers -- New methods for reverse transfection with siRNA from a solid surface / Satoshi Fujita [and others] -- Nonviral siRNA delivery for gene silencing in neurodegenerative diseases / Satya Prakash, Meenakshi Malhotra, and Venkatesh Rengaswamy.
Using siRNA to uncover novel oncogenic signaling pathways / Jin-Mei Lai, Chi-Ying F. Huang, and Chang-Han Chen -- Biodegradable polymer-mediated sh/siRNA delivery for cancer studies / Dhananjay J. Jere and Chong-Su Cho -- Cellular siRNA delivery using TatU1A and photo-induced RNA interference / Tamaki Endoh and Takashi Ohtsuki -- Polyethylenimine (PEI)/siRNA-mediated gene knockdown in vitro and in vivo / Sabrina Höbel and Achim Aigner -- Transfection of siRNAs in multiple myeloma cell lines / Jose L.R. Brito, Nicola Brown, and Garth J. Morgan -- A new approach for therapeutic use by RNA interference in the brain / Yukio Akaneya -- Inhibitory RNA molecules in immunotherapy for cancer / Chih-Ping Mao and T.-C. Wu -- Preventing tissue injury using siRNA / Zhu-Xu Zhang [and others] -- Preventing immune rejection through gene silencing / Xusheng Zhang, Mu Li, and Wei-Ping Min -- Topical application of siRNA targeting cutaneous dendritic cells in allergic skin disease / Miyuki Azuma, Patcharee Ritprajak, and Masaaki Hashiguchi -- Direct application of siRNA for in vivo pain research / Philippe Sarret, Louis Doré-Savard, and Nicolas Beaudet -- A potential therapeutic for pandemic influenza using RNA interference / Shaguna Seth [and others] -- Evaluation of targets for ovarian cancer gene silencing therapy : in vitro and in vivo approaches / Anastasia Malek and Oleg Tchernitsa.
Summary: From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace. In RNA Interference: From Biology to Clinical Applications, expert contributors provide an overview of the most current science and protocols that span the biological disciplines from detailed nucleic acid chemistry, to pharmacology, to the manipulation of signal transduction pathways. Divided into three distinct sections, this volume delves into the physiology of RNA interference, RNA interference in the laboratory and siRNA delivery, and preclinical and clinical issues associated with the use of RNAi-inducing agents as drugs in order to stimulate new questions and offer the tools necessary to start addressing those questions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and inspiring, RNA Interference: From Biology to Clinical Applications aims to promote and motivate innovation by reviewing what has been done, providing details of how it has been done, and encouraging speculation on what the future may hold.
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Includes bibliographical references and index.

Endogenous antiviral mechanisms of RNA interference : a comparative biology perspective / Abubaker M.E. Sidahmed and Bruce Wilkie -- Monitoring innate immune recruitment by siRNAs in mammalian cells / Michael P. Gantier and Bryan R.G. Williams -- Current knowledge of microRNAs and noncoding RNAs in virus-infected cells / Dominique L. Ouellet and Patrick Provost -- Allele-specific silencing by RNA interference / Hirohiko Hohjoh -- Computational siRNA design considering alternative splicing / Young J. Kim -- Bioinformatic approaches to siRNA selection and optimization / Pirkko Muhonen and Harry Holthofer -- Optimized gene silencing by co-expression of mulitple shRNAs in a single vector / Yasuhito Ishigaki, Akihiro Nagao, and Tsukasa Matsunaga -- Strategies in designing multigene expression units to downregulate HIV-1 / Jane Zhang and John J. Rossi -- Designing optimal siRNA based on target site accessibility / Ivo L. Hofacker and Hakim Tafer -- Chemical synthesis of 2'-O-alkylated siRNAs / Joachim W. Engels [and others] -- siRNA specific delivery system for targeting dendritic cells / Xiufen Zheng [and others] -- Hydrodynamic delivery protocols / Piotr G. Rychahou and B. Mark Evers -- New methods for reverse transfection with siRNA from a solid surface / Satoshi Fujita [and others] -- Nonviral siRNA delivery for gene silencing in neurodegenerative diseases / Satya Prakash, Meenakshi Malhotra, and Venkatesh Rengaswamy.

Using siRNA to uncover novel oncogenic signaling pathways / Jin-Mei Lai, Chi-Ying F. Huang, and Chang-Han Chen -- Biodegradable polymer-mediated sh/siRNA delivery for cancer studies / Dhananjay J. Jere and Chong-Su Cho -- Cellular siRNA delivery using TatU1A and photo-induced RNA interference / Tamaki Endoh and Takashi Ohtsuki -- Polyethylenimine (PEI)/siRNA-mediated gene knockdown in vitro and in vivo / Sabrina Höbel and Achim Aigner -- Transfection of siRNAs in multiple myeloma cell lines / Jose L.R. Brito, Nicola Brown, and Garth J. Morgan -- A new approach for therapeutic use by RNA interference in the brain / Yukio Akaneya -- Inhibitory RNA molecules in immunotherapy for cancer / Chih-Ping Mao and T.-C. Wu -- Preventing tissue injury using siRNA / Zhu-Xu Zhang [and others] -- Preventing immune rejection through gene silencing / Xusheng Zhang, Mu Li, and Wei-Ping Min -- Topical application of siRNA targeting cutaneous dendritic cells in allergic skin disease / Miyuki Azuma, Patcharee Ritprajak, and Masaaki Hashiguchi -- Direct application of siRNA for in vivo pain research / Philippe Sarret, Louis Doré-Savard, and Nicolas Beaudet -- A potential therapeutic for pandemic influenza using RNA interference / Shaguna Seth [and others] -- Evaluation of targets for ovarian cancer gene silencing therapy : in vitro and in vivo approaches / Anastasia Malek and Oleg Tchernitsa.

Print version record.

From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace. In RNA Interference: From Biology to Clinical Applications, expert contributors provide an overview of the most current science and protocols that span the biological disciplines from detailed nucleic acid chemistry, to pharmacology, to the manipulation of signal transduction pathways. Divided into three distinct sections, this volume delves into the physiology of RNA interference, RNA interference in the laboratory and siRNA delivery, and preclinical and clinical issues associated with the use of RNAi-inducing agents as drugs in order to stimulate new questions and offer the tools necessary to start addressing those questions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and inspiring, RNA Interference: From Biology to Clinical Applications aims to promote and motivate innovation by reviewing what has been done, providing details of how it has been done, and encouraging speculation on what the future may hold.

English.

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