Ligand-macromolecular interactions in drug discovery : methods and protocols / edited by Ana Cećilia A. Roque.
Contributor(s): Roque, Ana Cecília A. (Ana Cecília Afonso)Material type: TextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 572.Publisher: New York, NY : Humana Press, ©2010Description: 1 online resource (x, 297 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781607612445; 1607612445Subject(s): Drug development | Ligand binding (Biochemistry) | Macromolecules | Clinical Laboratory Techniques | Ligands | Macromolecular Substances | Drug Discovery | Chemistry, Pharmaceutical | Laboratory Chemicals | Investigative Techniques | Chemicals and Drugs | Pharmacology | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Specialty Uses of Chemicals | Chemistry | Chemical Actions and Uses | Biological Science Disciplines | Natural Science Disciplines | Disciplines and Occupations | Drug development | Ligand binding (Biochemistry) | Macromolecules | Pharmacy, Therapeutics, & Pharmacology | Health & Biological Sciences | farmacologie | pharmacology | toxicologie | toxicology | chemie | chemistry | geneeskunde | medicine | biomedische wetenschappen | biomedicine | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Ligand-macromolecular interactions in drug discovery.DDC classification: 615/.19 LOC classification: RM301.25 | .L54 2010Online resources: Click here to access online
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Includes bibliographical references and index.
Part I. Reviews -- 1. An Historical overview of drug discovery / Ana Sofia Pina, Abid Hussain, and Ana Cecília Roque -- 2. Ligand mcromolecule iteractions : theoretical principles of molecular recognition / Tammy Nolan, Nidhi Singh, and Christopher R. McCurdy -- 3. X-Ray cystallography in drug discovery / Ana Luísa Carvalho, José Trincão, and Maria João Romão -- 4. Virtual screening of compound libraries / Nuno M.F.S.A. Cerqueira, Sérgio F. Sousa, Pedro A. Fernandes, and Maria João Ramos -- Part II: Protocols -- 5. Combinatorial chemistry and the synthesis of compound libraries / Rolf Breinbauer and Matthias Mentel -- 6. Ligand-based nuclear magnetic resonance screening techniques / Aldino Viegas, Anjos L. Macedo, and Eurico Cabrita -- 7. Isothermal titration calorimetry and differential scanning calorimetry / Geoff Holdgate -- 8. Adaptive combinatorial design of focused compound libraries / Gisbert Schneider and Andreas Schüller -- 9. Chemical microarrays : a new tool for discovery enzyme inhibitors / Shuguang Liang, Wei Xu, Kurumi Y. Horiuchi, Yuan Wang, and Haiching Ma -- 10. Fluorescence polarization and time-resolved fluorescence resonance energy transfer techniques for PI3K assays / Kurumi Y. Horiuchi and Haiching Ma -- 11. Small molecule protein interaction profiling with functional protein microarrays / Lihao Meng, Dawn Mattoon, and Paul Predki -- 12. Capillary electrophoresis in drug discovery / Milena Quaglia and Ersilia De Lorenzi -- 13. SPR in drug discovery : searching bioactive compounds in plant extracts / Maria Minunni and Anna Rita Bilia -- 14. Application of frontal affinity chromatography with mass spectrometry (FAC-MS) for stereospecific ligand-macromolecule interaction, detection and screening / Jacek Slon-Usakiewicz and Peter Redden -- 15. GPC spin column HPLC-ESI-MS methods for screening drugs noncovalently bound to proteins / Marshall M. Siegel -- 16. A scintillation proximity assay for fatty acid amide hydrolase compatible with inhibitor screening / Yuren Wang and Philip Jones -- 17. A natural products approach to drug discovery : probing modes of action of antitumor agents by genome-scale cDNA library screening / Hendrik Luesch and Pedro Abreu -- 18. Ligand-macromolecule interactions in live cells by fluorescence correlation spectroscopy / Aladdin Pramanik.
Print version record.
Drug research has been greatly transformed by the "omics revolution" and advances in computational tools, combinatorial chemistry, and high throughput screening techniques (HTS). These implements have advanced the ability to identify target molecules that serve as points of attack for future medicines, the design and synthesis of potential lead compounds, and further characterization, screening, and assays for therapeutic efficacy and toxicity. In Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols, experts in the field highlight the main principles and methodologies currently utilized in the study of molecular interactions between compounds, either natural or synthetic, and complementary biological targets, within the scope of drug discovery. Beginning with a historical perspective of drug research focusing on the contribution of genomics, proteomics, high-throughput methods, and computational developments in drug discovery, the book then delves into highly detailed methods on topics such as NMR spectroscopy, compound library design and synthesis, chemical and biological microarrays, and many others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols will serve as an ideal guide to scientists in academia and in industry who are striving to further our knowledge of medicines.