Mass spectrometry imaging : principles and protocols / edited by Stanislav S. Rubakhin, Jonathan V. Sweedler.

Contributor(s): Rubakhin, Stanislav S | Sweedler, Jonathan V
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 656.; Springer protocols (Series): Publisher: Totowa, N.J. : London : Humana ; Springer [distributor], ©2010Description: 1 online resource (xiv, 487 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781607617464; 1607617463Subject(s): Mass spectrometry -- Methodology | Imaging systems | Mass Spectrometry -- methods | Imaging systems | Chemistry | Human Anatomy & Physiology | Physical Sciences & Mathematics | Health & Biological Sciences | Analytical Chemistry | Animal Biochemistry | geneeskunde | medicine | volksgezondheid | public health | afbeelden | imagery | spectrometrie | spectrometry | microscopie | microscopy | radiologie | radiology | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Mass spectrometry imaging.DDC classification: 543.65 LOC classification: QP519.9.M3Online resources: Click here to access online
Contents:
Imaging mass spectrometry : viewing the future -- A mass spectrometry primer for mass spectrometry imaging -- Imaging of metals, metalloids, and non-metals by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in biological tissues -- Lipid detection, identification, and imaging single cells with SIMS -- The application and potential of ion mobility mass spectrometry in imaging MS with a focus on lipids -- Quantitative imaging of chemical composition in single cells by secondary ion mass spectrometry : cisplatin affects calcium stores in renal epithelial cells -- Imaging MALDI mass spectrometry of sphingolipids using an oscillating capillary nebulizer matrix application system -- Mapping pharmaceuticals in rat brain sections using MALDI imaging mass spectrometry -- Laser ablation electrospray ionization for atmospheric pressure molecular imaging mass spectrometry -- Matrix-assisted laser desorption/ionization and nanoparticle-based imaging mass spectrometry for small metabolites : a practical protocol -- Cellular imaging using matrix-enhanced and metal-assisted SIMS -- Tandem mass spectrometric methods for phospholipid analysis from brain tissue -- Chemical imaging with desorption electrospray ionization mass spectrometry -- Mass spectrometry imaging of small molecules using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS) -- Preparation of single cells for imaging mass spectrometry -- Applying imaging ToF-SIMS and PCA in differentiation of tissue types -- Direct molecular analysis of whole-body animal tissue sections by MALDI imaging mass spectrometry -- MALDI direct analysis and imaging of frozen versus FFPE tissues : what strategy for which sample? -- On tissue protein identification improvement by N-terminal peptide derivatization -- Specific MALDI-MSI : Tag-Mass -- Structurally selective imaging mass spectrometry by imaging ion mobility-mass spectrometry -- Tutorial : multivariate statistical treatment of imaging data for clinical biomarker discovery -- Applications of MALDI-MSI to pharmaceutical research -- Tissue preparation for the in situ MALDI MS imaging of proteins, lipids, and small molecules at cellular resolution -- Imaging of similar mass neuropeptides in neuronal tissue by enhanced resolution MALDI MS with an ion trap -- Orbitrap hybrid instrument -- Mass spectrometric imaging of neuropeptides in decapod crustacean neuronal tissues -- Mass spectrometry imaging using the stretched sample approach.
Summary: Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular Biology series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting. Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.
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Includes bibliographical references and index.

Imaging mass spectrometry : viewing the future -- A mass spectrometry primer for mass spectrometry imaging -- Imaging of metals, metalloids, and non-metals by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in biological tissues -- Lipid detection, identification, and imaging single cells with SIMS -- The application and potential of ion mobility mass spectrometry in imaging MS with a focus on lipids -- Quantitative imaging of chemical composition in single cells by secondary ion mass spectrometry : cisplatin affects calcium stores in renal epithelial cells -- Imaging MALDI mass spectrometry of sphingolipids using an oscillating capillary nebulizer matrix application system -- Mapping pharmaceuticals in rat brain sections using MALDI imaging mass spectrometry -- Laser ablation electrospray ionization for atmospheric pressure molecular imaging mass spectrometry -- Matrix-assisted laser desorption/ionization and nanoparticle-based imaging mass spectrometry for small metabolites : a practical protocol -- Cellular imaging using matrix-enhanced and metal-assisted SIMS -- Tandem mass spectrometric methods for phospholipid analysis from brain tissue -- Chemical imaging with desorption electrospray ionization mass spectrometry -- Mass spectrometry imaging of small molecules using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS) -- Preparation of single cells for imaging mass spectrometry -- Applying imaging ToF-SIMS and PCA in differentiation of tissue types -- Direct molecular analysis of whole-body animal tissue sections by MALDI imaging mass spectrometry -- MALDI direct analysis and imaging of frozen versus FFPE tissues : what strategy for which sample? -- On tissue protein identification improvement by N-terminal peptide derivatization -- Specific MALDI-MSI : Tag-Mass -- Structurally selective imaging mass spectrometry by imaging ion mobility-mass spectrometry -- Tutorial : multivariate statistical treatment of imaging data for clinical biomarker discovery -- Applications of MALDI-MSI to pharmaceutical research -- Tissue preparation for the in situ MALDI MS imaging of proteins, lipids, and small molecules at cellular resolution -- Imaging of similar mass neuropeptides in neuronal tissue by enhanced resolution MALDI MS with an ion trap -- Orbitrap hybrid instrument -- Mass spectrometric imaging of neuropeptides in decapod crustacean neuronal tissues -- Mass spectrometry imaging using the stretched sample approach.

Print version record.

Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular Biology series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting. Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.

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