MicroRNA and cancer : methods and protocols / edited by Wei Wu.

Contributor(s): Wu, Wei
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 676.Publisher: New York, N.Y. : Humana Press, ©2011Description: 1 online resource (x, 273 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781607618638; 160761863XSubject(s): Small interfering RNA | Cancer | Biology | RNA, Antisense | Publication Formats | Disease | RNA, Small Untranslated | Biological Science Disciplines | RNA, Untranslated | Antisense Elements (Genetics) | RNA | Publication Characteristics | Neoplasms | MicroRNAs | Genetics | Laboratory Manuals | Nucleic Acids, Nucleotides, and Nucleosides | Nucleic Acids | Natural Science Disciplines | Chemicals and Drugs | Disciplines and Occupations | Cancer | Small interfering RNA | neoplasms | biomedische wetenschappen | biomedicine | humane genetica | human genetics | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: MicroRNA and cancer.DDC classification: 572.8/8 LOC classification: QP623.5.S63 | M53 2011Online resources: Click here to access online
Contents:
MicroRNA biogenesis and cancer / J. Winter and S. Diederichs -- Computational identification of miRNAs involved in cancer / A. Oulas, N. Karathanasis and P. Poirazi -- The principles of miRNA-masking antisense oligonucleotides technology / Z. Wang -- The concept of multiple-target anti-miRNA antisense oligonucleotide technology / Z. Wang -- Modulation of microRNAs for potential cancer therapeutics / W. Wu -- Detection of microRNAs in cultured cells and paraffin-embedded tissue specimens by in situ hybridization / A. Gupta and Y.Y. Mo -- MicroRNA northern blotting, precursor cloning, and Ago2-improved rna interference / J. Winter and S. Diederichs -- MiRNA profiling on high-throughput openarray system / E.V. Grigorenko [and others] -- Silicon nanowire biosensor for ultrasensitive and label-free direct detection of miRNAs / G.J. Zhang -- High-throughput and reliable protocols for animal microRNA library cloning / C. Xiao -- MicroRNA regulation of growth factor receptor signaling in human cancer cells / K.M. Giles [and others] -- Epigenetic regulation of microRNA expression in cancer / H. Choudhry and J.W. Catto -- In vitro functional study of miR-126 in leukemia / Z. Li and J. Chen -- Prediction of the biological effect of polymorphisms within microRNA binding sites / D. Landi [and others] -- The guideline of the design and validation of miRNA mimics / Z. Wang -- Analysis of targets and functions coregulated by microRNAs / S.J. Chen and H.C. Chen -- Utilization of SSCprofiler to predict a new miRNA gene / A. Oulas and P. Poirazi -- MicroRNA profiling using fluorescence-labeled beads: Data acquisition and processing / T. Streichert, B. Otto and U. Lehmann.
Summary: The tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes. Recent miRNA studies change the landscape of cancer genetics by scrutinizing the alterations of genome-wide miRNA expressions in most common cancers and their regulatory functions during the development of cancer. The connections between miRNAs and cancer are widespread enough to warrant more comprehensive investigations in the systems biology perspective. In MicroRNA and Cancer: Methods and Protocols, internationally renowned experts provide the latest miRNA knowledge, the various techniques and methodologies currently available for cancer research application. Ranging from the fundamental concepts to practical applications, this book presents: • Overview of microRNA biogenesis, computational prediction of new miRNAs in the cancer genome, and miRNA-based therapeutic approaches for cancer treatment • Detailed experimental protocols in miRNA detection with novel and high-throughput technology, miRNA library cloning, miRNA epigenetic regulation, and miRNA pathway study • Stepwise computational and bioinformatic procedures for miRNA complex networks in cancer genomes with a variety of softwares and programs • Cross-cited notes on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols serves researchers with the basic principles of experimental and computational methods for microRNA study in cancer research and provides a firm grounding for those who wish to further develop their own applications and tailor them to their own specific research needs.
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Includes bibliographical references and index.

MicroRNA biogenesis and cancer / J. Winter and S. Diederichs -- Computational identification of miRNAs involved in cancer / A. Oulas, N. Karathanasis and P. Poirazi -- The principles of miRNA-masking antisense oligonucleotides technology / Z. Wang -- The concept of multiple-target anti-miRNA antisense oligonucleotide technology / Z. Wang -- Modulation of microRNAs for potential cancer therapeutics / W. Wu -- Detection of microRNAs in cultured cells and paraffin-embedded tissue specimens by in situ hybridization / A. Gupta and Y.Y. Mo -- MicroRNA northern blotting, precursor cloning, and Ago2-improved rna interference / J. Winter and S. Diederichs -- MiRNA profiling on high-throughput openarray system / E.V. Grigorenko [and others] -- Silicon nanowire biosensor for ultrasensitive and label-free direct detection of miRNAs / G.J. Zhang -- High-throughput and reliable protocols for animal microRNA library cloning / C. Xiao -- MicroRNA regulation of growth factor receptor signaling in human cancer cells / K.M. Giles [and others] -- Epigenetic regulation of microRNA expression in cancer / H. Choudhry and J.W. Catto -- In vitro functional study of miR-126 in leukemia / Z. Li and J. Chen -- Prediction of the biological effect of polymorphisms within microRNA binding sites / D. Landi [and others] -- The guideline of the design and validation of miRNA mimics / Z. Wang -- Analysis of targets and functions coregulated by microRNAs / S.J. Chen and H.C. Chen -- Utilization of SSCprofiler to predict a new miRNA gene / A. Oulas and P. Poirazi -- MicroRNA profiling using fluorescence-labeled beads: Data acquisition and processing / T. Streichert, B. Otto and U. Lehmann.

Print version record.

The tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes. Recent miRNA studies change the landscape of cancer genetics by scrutinizing the alterations of genome-wide miRNA expressions in most common cancers and their regulatory functions during the development of cancer. The connections between miRNAs and cancer are widespread enough to warrant more comprehensive investigations in the systems biology perspective. In MicroRNA and Cancer: Methods and Protocols, internationally renowned experts provide the latest miRNA knowledge, the various techniques and methodologies currently available for cancer research application. Ranging from the fundamental concepts to practical applications, this book presents: • Overview of microRNA biogenesis, computational prediction of new miRNAs in the cancer genome, and miRNA-based therapeutic approaches for cancer treatment • Detailed experimental protocols in miRNA detection with novel and high-throughput technology, miRNA library cloning, miRNA epigenetic regulation, and miRNA pathway study • Stepwise computational and bioinformatic procedures for miRNA complex networks in cancer genomes with a variety of softwares and programs • Cross-cited notes on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols serves researchers with the basic principles of experimental and computational methods for microRNA study in cancer research and provides a firm grounding for those who wish to further develop their own applications and tailor them to their own specific research needs.

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