Serum/plasma proteomics : methods and protocols / edited by Richard J. Simpson and David W. Greening.
Contributor(s): Simpson, Richard J | Greening, David WMaterial type: TextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 728.Publisher: New York, N.Y. : Humana Press, ©2011Description: 1 online resource (xiv, 397 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781617790683; 1617790680Other title: Plasma proteomicsSubject(s): Serum | Blood plasma | Proteomics | Serum | Plasma | Blood Proteins | Proteomics -- methods | Blood plasma | Proteomics | Serum | Chemistry | Human Anatomy & Physiology | Physical Sciences & Mathematics | Health & Biological Sciences | Physiology | Biochemistry | eiwitten | proteins | hematologie | haematology | eiwitexpressieanalyse | proteomics | ProteomicsGenre/Form: Electronic books. Additional physical formats: Print version:: Serum/plasma proteomics.DDC classification: 573.1/56 LOC classification: QP91Online resources: Click here to access online
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Includes bibliographical references and index.
Plasma biomarker discovery using 3D protein profiling coupled with label-free quantitation / Lynn A. Beer [and others] -- Intact protein separation by one- and two-dimensional liquid chromatography for the comparative proteomic separation of partitioned serum or plasma / Simon Sheng [and others] -- In-depth analysis of a plasma or serum proteome using a 4D protein profiling method / Hsin-Yao Tang, Lynn A. Beer, and David W. Speicher -- Intact-protein analysis system for discovery of serum-based disease biomarkers / Hong Wang and Samir Hanash -- Model-based discovery of circulating biomarkers / Maryann S. Vogelsang [and others] -- Low-molecular weight plasma proteome analysis using centrifugal ultrafiltration / David W. Greening and Richard J. Simpson -- High-throughput analysis of glycoproteins from plasma / Yan Li and Hui Zhang -- Minimizing preanalytical variation of plasma samples by proper blood collection and handling / Jizu Yi, David Craft, and Craig A. Gelfand -- Collection and handling of blood specimens for peptidomics / Harald Tammen and Rüdiger Hess -- Investigation of peptide biomarker stability in plasma samples using time-course MS analysis / Jizu Yi [and others] -- Biomarker validation in blood specimens by selected reaction monitoring mass spectrometry of N-glycosites / Reto Ossola [and others] -- Fluorescent microsphere-based method for assay of multiple analytes in plasma / Oliver K. Bernhard [and others] -- Immuno-mass spectrometry : quantification of low-abundance proteins in biological fluids / Vathany Kulasingam [and others] -- Qualitative and quantitative analysis of glycated proteins in human plasma by glucose isotopic labeling with 13C6-reducing sugars / Feliciano Priego-Capote [and others] -- Exosome isolation for proteomic analyses and RNA profiling / Douglas D. Taylor, Wolfgang Zacharias, and Cicek Gercel-Taylor -- Extraction and proteome analysis of liver tissue interstitial fluid / Wei Sun, Ying Jiang, and Fuchu He -- Protocol for the preparation of cryoprecipitate and cryodepleted plasma / Rosemary L. Sparrow, David W. Greening, and Richard J. Simpson -- Preparation of platelet concentrates / David W. Greening, Rosemary L. Sparrow, and Richard J. Simpson -- Phosphoproteome analysis of the platelet plasma membrane / Thomas Premsler [and others] -- Statistical design and analysis of label-free LC-MS proteomic experiments : a case study of coronary artery disease / Timothy Clough [and others] -- Data management in mass spectrometry-based proteomics / Lennart Martens -- Bioinformatics challenges in the proteomic analysis of human plasma / Joseph M. Foster and Lennart Martens -- Using the human plasma peptide atlas to study human plasma proteins / Terry Farrah, Eric W. Deutsch, and Ruedi Aebersold.
Print version record.
Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome. These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Serum/Plasma Proteomics: Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to the field in an effort to further our knowledge of this fundamental science.
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