Yeast genetic networks : methods and protocols / edited by Attila Becskei.

Contributor(s): Becskei, Attila
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 734.Publisher: New York, N.Y. : Humana Press, ©2011Description: 1 online resource (xi, 344 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781617790867; 1617790869Subject(s): Yeast -- Genetics | Biology | Genetic Techniques | Models, Biological | Fungi | Investigative Techniques | Biological Science Disciplines | Models, Theoretical | Eukaryota | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Organisms | Natural Science Disciplines | Disciplines and Occupations | Yeasts | Genetics | Models, Genetic | Gene Expression Profiling | Yeast -- Genetics | Biology | Botany | Health & Biological Sciences | Earth & Environmental Sciences | Fungi & Algae | Microbiology & Immunology | gisten | yeasts | microbiologie | microbiology | genexpressie | gene expression | genetica | genetics | Genetics (General) | Genetica (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Yeast genetic networks.DDC classification: 660.6/2 LOC classification: QK617.5 | .Y43 2011Online resources: Click here to access online
Contents:
Global estimation of mRNA stability in yeast / Julia Marin-Navarro [and others] -- Genomic-wide methods to evaluate transcription rates in yeast / Jose Garcia-Martinez, Vicent Pelechano, and Jose E. Perez-Ortin -- Construction of cis-regulatory input functions of yeast promoters / Prasuna Ratna and Attila Becskei -- Luminescence as a continuous real-time reporter of promoter activity in yeast undergoing respiratory oscillations or cell division rhythms / J. Brian Robertson and Carl Hirschie Johnson -- Linearizer gene circuits with negative feedback regulation / Dmitry Nevozhay, Rhys M. Adams, and Gabor Balazsi -- Measuring in vivo signaling kinetics in a mitogen-activated kinase pathway using dynamic input stimulation / Megan N. McClean, Pascal Hersen, and Sharad Ramanathan -- Stochastic analysis of gene expression / Xiu-Deng Zheng and Yi Tao -- Studying adaptation and homeostatic behaviors of kinetic networks by using MATLAB / Tormod Drengstig, Thomas Kjosmoen, and Peter Ruoff -- Biochemical systems analysis of signaling pathways to understand fungal pathogenicity / Jacqueline Garcia [and others] -- Clustering change patterns using fourier transformation with time-course gene expression data / Jaehee Kim -- Finding modulators of stochasticity levels by quantitative genetics / Steffen Fehrmann and Gael Yvert -- Functional mapping of expression quantitative trait loci that regulate oscillatory gene expression / Arthur Berg [and others] -- Evolutionary aspects of a genetic network : studying the lactose/galactose regulon of Kluyveromyces lactis / Alexander Anders and Karin D. Breunig -- Analysis of subtelomeric silencing in Candida glabrata / Alejandro Juarez-Reyes, Alejandro De Las Penas, and Irene Castano -- Morphological and molecular genetic analysis of epigenetic switching of the human fungal pathogen Candida albicans / Denes Hnisz, Michael Tscherner, and Karl Kuchler -- Quantitation of cellular components in Cryptococcus neoformans for system biology analysis / Arpita Singh, Asfia Qureshi, and Maurizio Del Poeta.
Summary: Regulation of gene expression, a major determinant of gene activity, occupies a central place in molecular biology. Yeast Genetic Networks: Methods and Protocols covers approaches to the systems biological analysis of small-scale gene networks in yeast. Divided into four convenient sections, this detailed volume discusses the methods used to analyze gene expression quantitatively, presents a collection of mathematical and computational tools to analyze stochasticity, adaptation, sensitivity in signal transmission, and oscillations in gene expression, provides instructional methods on how to utilize the tools of quantitative genetics to identify genes that regulate stochasticity and oscillations in gene expression, and concludes with a section devoted to the analysis of conserved gene expression systems and networks in different fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Yeast Genetic Networks: Methods and Protocols serves as an ideal and valuable resource for both novices and experienced researchers.
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Includes bibliographical references and index.

Global estimation of mRNA stability in yeast / Julia Marin-Navarro [and others] -- Genomic-wide methods to evaluate transcription rates in yeast / Jose Garcia-Martinez, Vicent Pelechano, and Jose E. Perez-Ortin -- Construction of cis-regulatory input functions of yeast promoters / Prasuna Ratna and Attila Becskei -- Luminescence as a continuous real-time reporter of promoter activity in yeast undergoing respiratory oscillations or cell division rhythms / J. Brian Robertson and Carl Hirschie Johnson -- Linearizer gene circuits with negative feedback regulation / Dmitry Nevozhay, Rhys M. Adams, and Gabor Balazsi -- Measuring in vivo signaling kinetics in a mitogen-activated kinase pathway using dynamic input stimulation / Megan N. McClean, Pascal Hersen, and Sharad Ramanathan -- Stochastic analysis of gene expression / Xiu-Deng Zheng and Yi Tao -- Studying adaptation and homeostatic behaviors of kinetic networks by using MATLAB / Tormod Drengstig, Thomas Kjosmoen, and Peter Ruoff -- Biochemical systems analysis of signaling pathways to understand fungal pathogenicity / Jacqueline Garcia [and others] -- Clustering change patterns using fourier transformation with time-course gene expression data / Jaehee Kim -- Finding modulators of stochasticity levels by quantitative genetics / Steffen Fehrmann and Gael Yvert -- Functional mapping of expression quantitative trait loci that regulate oscillatory gene expression / Arthur Berg [and others] -- Evolutionary aspects of a genetic network : studying the lactose/galactose regulon of Kluyveromyces lactis / Alexander Anders and Karin D. Breunig -- Analysis of subtelomeric silencing in Candida glabrata / Alejandro Juarez-Reyes, Alejandro De Las Penas, and Irene Castano -- Morphological and molecular genetic analysis of epigenetic switching of the human fungal pathogen Candida albicans / Denes Hnisz, Michael Tscherner, and Karl Kuchler -- Quantitation of cellular components in Cryptococcus neoformans for system biology analysis / Arpita Singh, Asfia Qureshi, and Maurizio Del Poeta.

Regulation of gene expression, a major determinant of gene activity, occupies a central place in molecular biology. Yeast Genetic Networks: Methods and Protocols covers approaches to the systems biological analysis of small-scale gene networks in yeast. Divided into four convenient sections, this detailed volume discusses the methods used to analyze gene expression quantitatively, presents a collection of mathematical and computational tools to analyze stochasticity, adaptation, sensitivity in signal transmission, and oscillations in gene expression, provides instructional methods on how to utilize the tools of quantitative genetics to identify genes that regulate stochasticity and oscillations in gene expression, and concludes with a section devoted to the analysis of conserved gene expression systems and networks in different fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Yeast Genetic Networks: Methods and Protocols serves as an ideal and valuable resource for both novices and experienced researchers.

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