Mammalian cell viability : methods and protocols / edited by Martin J. Stoddart.

Contributor(s): Stoddart, Martin J
Material type: TextTextSeries: Springer protocols (Series): ; Methods in molecular biology (Clifton, N.J.): v. 740.Publisher: New York : Springer, ©2011Description: 1 online resource (xi, 240 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781617791086; 1617791083Subject(s): Cells -- Physiology | Cells -- Analysis | Cells -- Aging | Cell death | Mammals -- Physiology | Cell Survival | Mammals -- physiology | Cell death | Cells -- Aging | Cells -- Analysis | Mammals -- Physiology | celbiologie | cellular biology | celkweek | cell culture | levenswetenschappen | life sciences | Cellular Biology | CelbiologieGenre/Form: Electronic books. Additional physical formats: Print version:: Mammalian cell viability.DDC classification: 571.6 LOC classification: QH582.4 | .M36 2011Online resources: Click here to access online
Contents:
Cell viability assays : introduction / Martin J. Stoddart -- Cell viability analysis using trypan blue : manual and automated methods / Kristine S. Louis and Andre C. Siegel -- Estimation of cell number based on metabolic activity : the MTT reduction assay / Laszlo Kupcsik -- WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells / Martin J. Stoddart -- Assessment of cell proliferation with resazurin-based fluorescent dye / Ewa M. Czekanska -- xCELLigence system for real-time and label-free monitoring of cell viability / Ning Ke [and others] -- Analysis of tumor and endothelial cell viability and survival using sulforhodamine B and clonogenic assays / Caroline Woolston and Stewart Martin -- Annexin V/7-AAD staining in keratinocytes / Maya Zimmermann and Norbert Meyer -- Measurement of caspase activity : from cell populations to individual cells / Gabriela Paroni and Claudio Brancolini -- Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes / Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz -- Multiplexing cell viability assays / Helga H.J. Gerets, Stephane Dhalluin, and Franck A. Atienzar -- Cytotoxicity testing : measuring viable cells, dead cells, and detecting mechanism of cell death / Terry L. Riss, Richard A. Moravec, and Andrew L. Niles -- Fluorescein diacetate for determination of cell viability in 3D fibroblast-collagen-GAG constructs / Heather M. Powell, Alexis D. Armour, and Steven T. Boyce -- Confocal imaging protocols for live/dead staining in three-dimensional carriers / Benjamin Gantenbein-Ritter . [and others] -- Viability assessment of osteocytes using histological lactate dehydrogenase activity staining on human cancellous bone sections / Katharina Jahn and Martin J. Stoddart -- Measuring glutamate receptor activation-induced apoptotic cell death in ischemic rat retina using the TUNEL assay / Won-Kyu Ju and Keun-Young Kim -- Exploiting the liberation of Zn2+ to measure cell viability / Christian J. Stork and Yang V. Li -- Noninvasive bioluminescent quantification of viable stem cells in engineered constructs / Karim Oudina, Adeline Cambon-Binder, and Delphine Logeart-Avramoglou -- Raman micro-spectroscopy as a non-invasive cell viability test / Sophie Verrier, Alina Zoladek, and Ioan Notingher -- Closed ampoule isothermal microcalorimetry for continuous real-time detection and evaluation of cultured mammalian cell activity and responses / Olivier Braissant and Alma U. "Dan" Daniels -- Digital image processing of live/dead staining / Pieter Spaepen [and others].
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Includes bibliographical references and index.

Cell viability assays : introduction / Martin J. Stoddart -- Cell viability analysis using trypan blue : manual and automated methods / Kristine S. Louis and Andre C. Siegel -- Estimation of cell number based on metabolic activity : the MTT reduction assay / Laszlo Kupcsik -- WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells / Martin J. Stoddart -- Assessment of cell proliferation with resazurin-based fluorescent dye / Ewa M. Czekanska -- xCELLigence system for real-time and label-free monitoring of cell viability / Ning Ke [and others] -- Analysis of tumor and endothelial cell viability and survival using sulforhodamine B and clonogenic assays / Caroline Woolston and Stewart Martin -- Annexin V/7-AAD staining in keratinocytes / Maya Zimmermann and Norbert Meyer -- Measurement of caspase activity : from cell populations to individual cells / Gabriela Paroni and Claudio Brancolini -- Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes / Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz -- Multiplexing cell viability assays / Helga H.J. Gerets, Stephane Dhalluin, and Franck A. Atienzar -- Cytotoxicity testing : measuring viable cells, dead cells, and detecting mechanism of cell death / Terry L. Riss, Richard A. Moravec, and Andrew L. Niles -- Fluorescein diacetate for determination of cell viability in 3D fibroblast-collagen-GAG constructs / Heather M. Powell, Alexis D. Armour, and Steven T. Boyce -- Confocal imaging protocols for live/dead staining in three-dimensional carriers / Benjamin Gantenbein-Ritter . [and others] -- Viability assessment of osteocytes using histological lactate dehydrogenase activity staining on human cancellous bone sections / Katharina Jahn and Martin J. Stoddart -- Measuring glutamate receptor activation-induced apoptotic cell death in ischemic rat retina using the TUNEL assay / Won-Kyu Ju and Keun-Young Kim -- Exploiting the liberation of Zn2+ to measure cell viability / Christian J. Stork and Yang V. Li -- Noninvasive bioluminescent quantification of viable stem cells in engineered constructs / Karim Oudina, Adeline Cambon-Binder, and Delphine Logeart-Avramoglou -- Raman micro-spectroscopy as a non-invasive cell viability test / Sophie Verrier, Alina Zoladek, and Ioan Notingher -- Closed ampoule isothermal microcalorimetry for continuous real-time detection and evaluation of cultured mammalian cell activity and responses / Olivier Braissant and Alma U. "Dan" Daniels -- Digital image processing of live/dead staining / Pieter Spaepen [and others].

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