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Mammalian cell viability : methods and protocols / edited by Martin J. Stoddart.

Contributor(s): Stoddart, Martin JMaterial type: TextTextSeries: Springer protocols (Series) | Methods in molecular biology (Clifton, N.J.) ; v. 740.Publication details: New York : Springer, ©2011. Description: 1 online resource (xi, 240 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781617791086; 1617791083Subject(s): Cells -- Physiology | Cells -- Analysis | Cells -- Aging | Cell death | Mammals -- Physiology | Cell Survival | Mammals -- physiology | Cell death | Cells -- Aging | Cells -- Analysis | Mammals -- Physiology | celbiologie | cellular biology | celkweek | cell culture | levenswetenschappen | life sciences | Cellular Biology | CelbiologieGenre/Form: Electronic books. Additional physical formats: Print version:: Mammalian cell viability.DDC classification: 571.6 LOC classification: QH582.4 | .M36 2011NLM classification: W1 | QU 25Online resources: Click here to access online
Contents:
Cell viability assays : introduction / Martin J. Stoddart -- Cell viability analysis using trypan blue : manual and automated methods / Kristine S. Louis and Andre C. Siegel -- Estimation of cell number based on metabolic activity : the MTT reduction assay / Laszlo Kupcsik -- WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells / Martin J. Stoddart -- Assessment of cell proliferation with resazurin-based fluorescent dye / Ewa M. Czekanska -- xCELLigence system for real-time and label-free monitoring of cell viability / Ning Ke [and others] -- Analysis of tumor and endothelial cell viability and survival using sulforhodamine B and clonogenic assays / Caroline Woolston and Stewart Martin -- Annexin V/7-AAD staining in keratinocytes / Maya Zimmermann and Norbert Meyer -- Measurement of caspase activity : from cell populations to individual cells / Gabriela Paroni and Claudio Brancolini -- Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes / Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz -- Multiplexing cell viability assays / Helga H.J. Gerets, Stephane Dhalluin, and Franck A. Atienzar -- Cytotoxicity testing : measuring viable cells, dead cells, and detecting mechanism of cell death / Terry L. Riss, Richard A. Moravec, and Andrew L. Niles -- Fluorescein diacetate for determination of cell viability in 3D fibroblast-collagen-GAG constructs / Heather M. Powell, Alexis D. Armour, and Steven T. Boyce -- Confocal imaging protocols for live/dead staining in three-dimensional carriers / Benjamin Gantenbein-Ritter . [and others] -- Viability assessment of osteocytes using histological lactate dehydrogenase activity staining on human cancellous bone sections / Katharina Jahn and Martin J. Stoddart -- Measuring glutamate receptor activation-induced apoptotic cell death in ischemic rat retina using the TUNEL assay / Won-Kyu Ju and Keun-Young Kim -- Exploiting the liberation of Zn2+ to measure cell viability / Christian J. Stork and Yang V. Li -- Noninvasive bioluminescent quantification of viable stem cells in engineered constructs / Karim Oudina, Adeline Cambon-Binder, and Delphine Logeart-Avramoglou -- Raman micro-spectroscopy as a non-invasive cell viability test / Sophie Verrier, Alina Zoladek, and Ioan Notingher -- Closed ampoule isothermal microcalorimetry for continuous real-time detection and evaluation of cultured mammalian cell activity and responses / Olivier Braissant and Alma U. "Dan" Daniels -- Digital image processing of live/dead staining / Pieter Spaepen [and others].
Summary: Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field
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Includes bibliographical references and index.

Cell viability assays : introduction / Martin J. Stoddart -- Cell viability analysis using trypan blue : manual and automated methods / Kristine S. Louis and Andre C. Siegel -- Estimation of cell number based on metabolic activity : the MTT reduction assay / Laszlo Kupcsik -- WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells / Martin J. Stoddart -- Assessment of cell proliferation with resazurin-based fluorescent dye / Ewa M. Czekanska -- xCELLigence system for real-time and label-free monitoring of cell viability / Ning Ke [and others] -- Analysis of tumor and endothelial cell viability and survival using sulforhodamine B and clonogenic assays / Caroline Woolston and Stewart Martin -- Annexin V/7-AAD staining in keratinocytes / Maya Zimmermann and Norbert Meyer -- Measurement of caspase activity : from cell populations to individual cells / Gabriela Paroni and Claudio Brancolini -- Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes / Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz -- Multiplexing cell viability assays / Helga H.J. Gerets, Stephane Dhalluin, and Franck A. Atienzar -- Cytotoxicity testing : measuring viable cells, dead cells, and detecting mechanism of cell death / Terry L. Riss, Richard A. Moravec, and Andrew L. Niles -- Fluorescein diacetate for determination of cell viability in 3D fibroblast-collagen-GAG constructs / Heather M. Powell, Alexis D. Armour, and Steven T. Boyce -- Confocal imaging protocols for live/dead staining in three-dimensional carriers / Benjamin Gantenbein-Ritter . [and others] -- Viability assessment of osteocytes using histological lactate dehydrogenase activity staining on human cancellous bone sections / Katharina Jahn and Martin J. Stoddart -- Measuring glutamate receptor activation-induced apoptotic cell death in ischemic rat retina using the TUNEL assay / Won-Kyu Ju and Keun-Young Kim -- Exploiting the liberation of Zn2+ to measure cell viability / Christian J. Stork and Yang V. Li -- Noninvasive bioluminescent quantification of viable stem cells in engineered constructs / Karim Oudina, Adeline Cambon-Binder, and Delphine Logeart-Avramoglou -- Raman micro-spectroscopy as a non-invasive cell viability test / Sophie Verrier, Alina Zoladek, and Ioan Notingher -- Closed ampoule isothermal microcalorimetry for continuous real-time detection and evaluation of cultured mammalian cell activity and responses / Olivier Braissant and Alma U. "Dan" Daniels -- Digital image processing of live/dead staining / Pieter Spaepen [and others].

Print version record.

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field

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