Immune receptors : methods and protocols / edited by Jonathan P. Rast, James W.D. Booth.

Includes bibliographical references and index.

Screening for ligands of c-type lectin-like receptors / Elwira Pyz and Gordon D. Brown -- Yeast surface display of lamprey variable lymphocyte receptors / Gang Xu, Satoshi Tasumi, and Zeev Pancer -- Identification of scavenger receptor ligands / Claudine Neyen, Annette Pluddemann, and Siamon Gordon -- Construction, expression, and purification of chimeric protein reagents based on immunoglobulin Fc regions / John P. Cannon, Marci O'Driscoll, and Gary W. Litman -- Innate immune receptors for nucleic acids / Andrea Stutz, Damien Bertheloot, and Eicke Latz -- Analysis of receptor-ligand interactions by surface plasmon resonance / Kimiko Kuroki and Katsumi Maenaka -- Cell-based reporter assay to analyze activation of Nod1 and Nod2 / Birte Zurek, Harald Bielig, and Thomas A. Kufer -- Determining FceRI diffusional dynamics via single quantum dot tracking / Diane S. Lidke [and others] -- Ratiometric analysis of subcellular recruitment of Fc receptors during phagocytosis / Patricia Mero and James W.D. Booth -- Assessment of the recycling of the membrane-bound chemokine, CX₃CL1 / Sajedabanu Patel, Ilya Mukovozov, and Lisa A. Robinson -- Measuring immune receptor mobility by fluorescence recovery after photobleaching / Kristen Silver and Rene E. Harrison -- Probing the plasma membrane structure of immune cells through the analysis of membrane sheets by electron microscopy / Bjorn F. Lillemeier and Mark M. Davis -- Rapamycin-based inducible translocation systems for studying phagocytosis / Michal Bohdanowicz and Gregory D. Fairn -- Micropatterned ligand arrays to study spatial regulation in Fc receptor signaling / Alexis J. Torres, David Holowka, and Barbara A. Baird -- CELLISA : reporter cell-based immunization and screening of hybridomas specific for cell surface antigens / Peter Chen, Aruz Mesci, and James R. Carlyle -- Use of colloidal silica-beads for the isolation of cell-surface proteins for mass spectrometry-based proteomics / Yunee Kim [and others] -- Transfection-based genomic readout for identifying rare transcriptional splice variants / Larry J. Dishaw [and others] -- Characterizing somatic hypermutation and gene conversion in the chicken DT40 cell system / Nagarama Kothapalli and Sebastian D. Fugmann -- Characterizing immune receptors from new genome sequences / Katherine M. Buckley and Jonathan P. Rast.

Print version record.

Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity. The receptors that mediate these functions are at the front lines of both protective and regulative roles of the immune system. In Immune Receptors: Methods and Protocols, expert researchers present a variety of experimental approaches to the characterization of immune receptors and the cell biology that mediates their functions. These include imaging techniques that aim to understand receptor localization and trafficking, techniques to measure receptor-ligand interactions, strategies to identify novel ligands and methods to analyze downstream receptor signaling, as well as strategies for genomic and proteomic characterization of receptor repertoires. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immune Receptors: Methods and Protocols offers technical descriptions and protocols that will be useful both to investigators who are interested in carrying out these procedures and to those who seek a deeper understanding of the bench science that lies behind the immunology literature.

English.