Therapeutic oligonucleotides : methods and protocols / edited by John Goodchild.

Contributor(s): Goodchild, John, chemist
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 764.; Springer protocols (Series): Publisher: New York : Humana Press : Springer, ©2011Description: 1 online resource (xi, 340 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781617791888; 1617791881Subject(s): Oligonucleotides -- Therapeutic use | Polynucleotides | Nucleotides | Nucleic Acids, Nucleotides, and Nucleosides | Chemicals and Drugs | Oligonucleotides | Oligonucleotides -- Therapeutic use | levenswetenschappen | life sciences | biochemie | biochemistry | nucleic acids | chemie | chemistry | Biology (General) | Biologie (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Therapeutic oligonucleotides.DDC classification: 572.8/633 LOC classification: QH345Online resources: Click here to access online
Contents:
Therapeutic oligonucleotides / John Goodchild -- Dinucleotides containing 3-S-phosphorothiolate linkages / James W. Gaynor and Richard Cosstick -- 2-O,4-C-methyleneoxymethylene bridged nucleic acids (2,4-BNACOC) / Yoshiyuki Hari [and others] -- Non-covalent peptide-based strategy for ex vivo and in vivo oligonucleotide delivery / Laurence Crombez [and others] -- Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides / Samir El Andaloussi [and others] -- Nanoparticle for tumor targeted delivery of oligomers / Xinrong Liu, Yi Wang, and Donald J. Hnatowich -- Light-directed delivery of nucleic acids / Sigurd Bøe [and others] -- Antibody targeted siRNA delivery / Masoud M. Toloue and Lance P. Ford -- Aptamer-drug conjugation for targeted tumor cell therapy / Michael J. Donovan [and others] -- Five-step process for screening antisense compounds for efficacy: gene target IL-12Rb2 / Nikki B. Marshall, Laura L. Hauck, and Dan V. Mourich -- Diverse small non-coding RNAs in RNA interference pathways / Liande Li and Yi Liu -- Quantification of siRNAs in vitro and in vivo / Angie Cheng, Alexander V. Vlassov, and Susan Magdaleno -- Optimization of transfection conditions and analysis of siRNA potency using real-time PCR / Angie Cheng, Susan Magdaleno, and Alexander V. Vlassov -- siRNA knockdown of gene expression in endothelial cells / Emily Dennstedt and Brad Bryan -- Using RNA interference in Schistosoma mansoni / Rita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly -- Performing the labeled microRNA pull-down (LAMP) assay system : an experimental approach for high-throughput identification of microRNA-target mRNAs / Ren-Jun Hsu and Huai-Jen Tsai -- Synthesis, purification, and characterization of oligoribonucleotides that act as agonists of TLR7 and/or TLR8 / Tao Lan and Ekambar R. Kandimalla -- Synthesis, purification, and characterization of immune-modulatory oligodeoxynucleotides that act as agonists of toll-like receptor 9 / Mallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla -- Surface plasmon resonance investigation of RNA aptamer-RNA ligand interactions / Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulme -- Practical considerations for analyzing antigene RNAs (agRNAs) : RNA immunoprecipitation of argonaute protein / Jacob C. Schwartz and David R. Corey -- Inhibition of human papillomavirus expression using DNAzymes / Maria Luisa Benitez-Hess, Pablo Reyes-Gutierrez, and Luis Marat Alvarez-Salas.
Summary: The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.
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Includes bibliographical references and index.

Therapeutic oligonucleotides / John Goodchild -- Dinucleotides containing 3-S-phosphorothiolate linkages / James W. Gaynor and Richard Cosstick -- 2-O,4-C-methyleneoxymethylene bridged nucleic acids (2,4-BNACOC) / Yoshiyuki Hari [and others] -- Non-covalent peptide-based strategy for ex vivo and in vivo oligonucleotide delivery / Laurence Crombez [and others] -- Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides / Samir El Andaloussi [and others] -- Nanoparticle for tumor targeted delivery of oligomers / Xinrong Liu, Yi Wang, and Donald J. Hnatowich -- Light-directed delivery of nucleic acids / Sigurd Bøe [and others] -- Antibody targeted siRNA delivery / Masoud M. Toloue and Lance P. Ford -- Aptamer-drug conjugation for targeted tumor cell therapy / Michael J. Donovan [and others] -- Five-step process for screening antisense compounds for efficacy: gene target IL-12Rb2 / Nikki B. Marshall, Laura L. Hauck, and Dan V. Mourich -- Diverse small non-coding RNAs in RNA interference pathways / Liande Li and Yi Liu -- Quantification of siRNAs in vitro and in vivo / Angie Cheng, Alexander V. Vlassov, and Susan Magdaleno -- Optimization of transfection conditions and analysis of siRNA potency using real-time PCR / Angie Cheng, Susan Magdaleno, and Alexander V. Vlassov -- siRNA knockdown of gene expression in endothelial cells / Emily Dennstedt and Brad Bryan -- Using RNA interference in Schistosoma mansoni / Rita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly -- Performing the labeled microRNA pull-down (LAMP) assay system : an experimental approach for high-throughput identification of microRNA-target mRNAs / Ren-Jun Hsu and Huai-Jen Tsai -- Synthesis, purification, and characterization of oligoribonucleotides that act as agonists of TLR7 and/or TLR8 / Tao Lan and Ekambar R. Kandimalla -- Synthesis, purification, and characterization of immune-modulatory oligodeoxynucleotides that act as agonists of toll-like receptor 9 / Mallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla -- Surface plasmon resonance investigation of RNA aptamer-RNA ligand interactions / Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulme -- Practical considerations for analyzing antigene RNAs (agRNAs) : RNA immunoprecipitation of argonaute protein / Jacob C. Schwartz and David R. Corey -- Inhibition of human papillomavirus expression using DNAzymes / Maria Luisa Benitez-Hess, Pablo Reyes-Gutierrez, and Luis Marat Alvarez-Salas.

The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.

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