Synthetic gene networks : methods and protocols / edited by Wilfried Weber, Martin Fussenegger.

Contributor(s): Weber, Wilfried, 1974- | Fussenegger, Martin
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 813.Publisher: New York : Humana Press, ©2012Description: 1 online resource (xi, 393 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781617794124; 1617794120; 1617794112; 9781617794117Subject(s): Synthetic biology -- Laboratory manuals | Bioengineering -- Laboratory manuals | Bioengineering | Regulatory Sequences, Nucleic Acid | Biology | Base Sequence | Engineering | Biological Science Disciplines | Natural Science Disciplines | Technology, Industry, and Agriculture | Genetic Structures | Genetic Phenomena | Disciplines and Occupations | Technology, Industry, Agriculture | Phenomena and Processes | Synthetic Biology | Gene Regulatory Networks | Bioengineering | Synthetic biology | Life sciences | Bioinformatics | rna | genen | genes | Bioinformatics (General) | Bioinformatica (algemeen)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Printed edition:: No titleDDC classification: 610.28 LOC classification: TA164 | .S96 2012Online resources: Click here to access online
Contents:
In silico implementation of synthetic gene networks -- Standardization in synthetic biology -- Robust optimal design of synthetic biological networks -- Predicting synthetic gene networks -- Reprogramming a GFP reporter gene subjects it to complex lentiviral gene regulation -- A high-throughput microfluidic method for generating and characterizing transcription factor mutant libraries -- Identifying and optimizing intracellular protein-protein interactions using bacterial genetic selection -- Zinc-finger nucleases-based genome engineering to generate isogenic human cell lines -- RNA-based networks: Using RNA aptamers and ribozymes as synthetic genetic devices -- MicroRNA circuits for transcriptional logic -- Light-regulated gene expression in yeast -- Light-controlled gene switches in mammalian cells -- Expressed protein modifications: Making synthetic proteins -- Using transcription machinery engineering to elicit complex cellular phenotypes -- Streamlining of a pseudomonas putida genome using a combinatorial deletion method based on minitransposon insertion and the flp-FRT recombination system -- Transposon-based and plasmid-based genetic tools for editing genomes of gram-negative bacteria -- Synthetic networks: Oscillators and toggle switches for escherichia coli -- Studying microbial communities in biofilms -- Quantitative analysis of the spatiotemporal dynamics of a synthetic predator-prey ecosystem -- Drosophila s2 schneider cells: A useful tool for rebuilding and redesigning approaches in synthetic biology -- Synthetic gene networks in plant systems -- Design and construction of synthetic gene networks in mammalian cells -- Synthetic gene networks as blueprint for smart hydrogels.
Summary: The rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research. Synthetic Gene Networks: Methods and Protocols provides the necessary information to design and construct synthetic gene networks in different host backgrounds. Divided into four convenient sections, this volume focuses on design concepts to devise synthetic gene networks and how mathematical models can be applied to the predictable engineering of desired network features. The volume continues by highlighting the construction and validation of biologic tools, describing strategies to optimize and streamline the host cell for optimized network performance, and covering how optimally designed gene networks can be implemented in a large variety of host cells ranging from bacteria over yeast and insect cells to plant and mammalian cell culture. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Gene Networks: Methods and Protocols serves as an invaluable resource for established biologists, engineers, and computer scientists or novices just entering into the rapidly growing field of synthetic biology.
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Includes bibliographical references and index.

In silico implementation of synthetic gene networks -- Standardization in synthetic biology -- Robust optimal design of synthetic biological networks -- Predicting synthetic gene networks -- Reprogramming a GFP reporter gene subjects it to complex lentiviral gene regulation -- A high-throughput microfluidic method for generating and characterizing transcription factor mutant libraries -- Identifying and optimizing intracellular protein-protein interactions using bacterial genetic selection -- Zinc-finger nucleases-based genome engineering to generate isogenic human cell lines -- RNA-based networks: Using RNA aptamers and ribozymes as synthetic genetic devices -- MicroRNA circuits for transcriptional logic -- Light-regulated gene expression in yeast -- Light-controlled gene switches in mammalian cells -- Expressed protein modifications: Making synthetic proteins -- Using transcription machinery engineering to elicit complex cellular phenotypes -- Streamlining of a pseudomonas putida genome using a combinatorial deletion method based on minitransposon insertion and the flp-FRT recombination system -- Transposon-based and plasmid-based genetic tools for editing genomes of gram-negative bacteria -- Synthetic networks: Oscillators and toggle switches for escherichia coli -- Studying microbial communities in biofilms -- Quantitative analysis of the spatiotemporal dynamics of a synthetic predator-prey ecosystem -- Drosophila s2 schneider cells: A useful tool for rebuilding and redesigning approaches in synthetic biology -- Synthetic gene networks in plant systems -- Design and construction of synthetic gene networks in mammalian cells -- Synthetic gene networks as blueprint for smart hydrogels.

Online resource; title from PDF title page (SpringerProtocols, viewed Nov. 30, 2011).

The rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research. Synthetic Gene Networks: Methods and Protocols provides the necessary information to design and construct synthetic gene networks in different host backgrounds. Divided into four convenient sections, this volume focuses on design concepts to devise synthetic gene networks and how mathematical models can be applied to the predictable engineering of desired network features. The volume continues by highlighting the construction and validation of biologic tools, describing strategies to optimize and streamline the host cell for optimized network performance, and covering how optimally designed gene networks can be implemented in a large variety of host cells ranging from bacteria over yeast and insect cells to plant and mammalian cell culture. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Gene Networks: Methods and Protocols serves as an invaluable resource for established biologists, engineers, and computer scientists or novices just entering into the rapidly growing field of synthetic biology.

English.

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