Next-generation MicroRNA expression profiling technology : methods and protocols / edited by Jian-Bing Fan.
Contributor(s): Fan, Jian-BingMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 822.Publisher: New York : Humana Press, ©2012Description: 1 online resource (xi, 341 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781617794278; 1617794279; 1617794260; 9781617794261Subject(s): Gene expression -- Laboratory manuals | RNA -- Laboratory manuals | Gene Expression Profiling | MicroRNAs | Gene expression | RNA | Medicine | Human genetics | Gene expression | Biomedicine | biomedische wetenschappen | humane genetica | genexpressie | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Printed edition:: No titleDDC classification: 572.8/65 LOC classification: QH450 | .N49 2012Online resources: Click here to access online
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Includes bibliographical references and index.
Controlling miRNA regulation in disease -- Introduction to miRNA profiling technologies and cross-platform comparison -- Stem-loop RT-qPCR for microRNA expression profiling -- Poly(T) adaptor rt-PCR -- MicroRNA in situ hybridization -- Agilent microRNA microarray profiling system -- MiRNA expression profiling using illumina universal beadchips -- MicroRNA expression analysis using the affymetrix platform -- Individualized miRNA assay panels using optically encoded beads -- Microfluidic primer extension assay -- MicroRNA profiling using microparaflo microfluidic array technology -- MicroRNA expression analysis using the illumina microRNA-seq platform -- Next-generation sequencing of miRNAs with roche 454 GS-FLX technology: Steps for a successful application -- Methods for small RNA preparation for digital gene expression profiling by next-generation sequencing -- Profiling of short RNAs using helicos single-molecule sequencing -- DeepBASE: Annotation and discovery of microRNAs and other noncoding RNAs from deep-sequencing data -- Phenomir: MicroRNAs in human diseases and biological processes -- MiRNA expression profiling: From reference genes to global mean normalization -- MiRNA data analysis: Next-gen sequencing -- Integrated miRNA expression analysis and target prediction -- MiRNAs in human cancer -- Blood-based miRNA preparation for noninvasive biomarker development.
The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today. This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.