Single-cell analysis : methods and protocols / edited by Sara Lindström, Helene Andersson-Svahn.

Contributor(s): Lindström, Sara | Andersson-Svahn, Helene
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 853.Publisher: New York : Humana Press, ©2012Description: 1 online resource (x, 238 pages)Content type: text Media type: computer Carrier type: online resourceISBN: 9781617795671; 1617795674; 1617795666; 9781617795664Subject(s): Cytology -- Laboratory manuals | Cytological Techniques | Clinical Laboratory Techniques | Investigative Techniques | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Single-Cell Analysis | Cytology | Life sciences | Cytology | Cell Biology | Cell Physiology | celbiologie | cellular biology | celfysiologie | Cellular Biology | CelbiologieGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Printed edition:: No titleDDC classification: 571.6 LOC classification: QH583.2 | .S56 2012Online resources: Click here to access online
Contents:
Introduction : why analyze single cells / Dino Di Carlo, Henry Tat Kwong Tse, and Daniel R. Gossett -- Flow cytometry and microscopy as means of studying single cells : a short introductional overview / Sara Lindstrom -- Identification of enzyme-converted peptide products from single cells using capillary electrophoresis and liquid chromatography-mass spectrometry / Robert B. Brown [and others] -- Laser capture microdissection for gene expression analysis of specific cell populations in single blastocysts / Ward De Spiegelaere, Muriel Filliers, and Ann Van Soom -- Single-cell culture in microwells / Sara Lindstrom and Helene Andersson-Svahn -- Isodielectric separation and analysis of cells / Michael D. Vahey and Joel Voldman -- Single cell electroporation using microfluidic devices / Severine Le Gac and Albert van den Berg -- Perfusion culture of mammalian cells in a microfluidic channel with a built-in pillar array / Chi Zhang -- Padlock probes and rolling circle amplification for detection of repeats and single-copy genes in the single-cell comet assay / Sara Henriksson and Mats Nilsson -- Droplet microfluidics for single-cell analysis / Eric Brouzes -- Screening of antigen-specific antibody-secreting cells / Hiroyuki Kishi [and others] -- Analysis of single eukaryotic cells using raman tweezers / Elsa Correia Faria and Peter Gardner -- Single-cell microinjection technologies / Yan Zhang -- Ultrasonic manipulation of single cells / Martin Wiklund and Bjorn Onfelt -- Expanding the horizons for single-cell applications on lab-on-a-chip devices / Soo Hyeon Kim, Dominique Fourmy, and Teruo Fujii -- Analytical technologies for integrated single-cell analysis of human immune responses / Ayca Yalcın, Yvonne J. Yamanaka, and J. Christopher Love.
Summary: Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail. The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.
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Includes index.

Introduction : why analyze single cells / Dino Di Carlo, Henry Tat Kwong Tse, and Daniel R. Gossett -- Flow cytometry and microscopy as means of studying single cells : a short introductional overview / Sara Lindstrom -- Identification of enzyme-converted peptide products from single cells using capillary electrophoresis and liquid chromatography-mass spectrometry / Robert B. Brown [and others] -- Laser capture microdissection for gene expression analysis of specific cell populations in single blastocysts / Ward De Spiegelaere, Muriel Filliers, and Ann Van Soom -- Single-cell culture in microwells / Sara Lindstrom and Helene Andersson-Svahn -- Isodielectric separation and analysis of cells / Michael D. Vahey and Joel Voldman -- Single cell electroporation using microfluidic devices / Severine Le Gac and Albert van den Berg -- Perfusion culture of mammalian cells in a microfluidic channel with a built-in pillar array / Chi Zhang -- Padlock probes and rolling circle amplification for detection of repeats and single-copy genes in the single-cell comet assay / Sara Henriksson and Mats Nilsson -- Droplet microfluidics for single-cell analysis / Eric Brouzes -- Screening of antigen-specific antibody-secreting cells / Hiroyuki Kishi [and others] -- Analysis of single eukaryotic cells using raman tweezers / Elsa Correia Faria and Peter Gardner -- Single-cell microinjection technologies / Yan Zhang -- Ultrasonic manipulation of single cells / Martin Wiklund and Bjorn Onfelt -- Expanding the horizons for single-cell applications on lab-on-a-chip devices / Soo Hyeon Kim, Dominique Fourmy, and Teruo Fujii -- Analytical technologies for integrated single-cell analysis of human immune responses / Ayca Yalcın, Yvonne J. Yamanaka, and J. Christopher Love.

Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail. The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.

Includes bibliographical reference and index.

English.

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