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Human embryonic stem cells handbook / edited by Kursad Turksen.

Contributor(s): Turksen, KursadMaterial type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; 873.Publication details: New York : Humana Press, ©2012. Description: 1 online resourceContent type: text Media type: computer Carrier type: online resourceISBN: 9781617797941; 1617797944; 1617797936; 9781617797934Subject(s): Embryonic stem cells -- Laboratory manuals | Publication Formats | Stem Cells | Cells | Publication Characteristics | Anatomy | Pluripotent Stem Cells | Embryonic Stem Cells | Laboratory Manuals | Embryonic stem cells | Life sciences | Cytology | Stem cells | Cell Biology | stamcellen | celbiologie | cellular biology | Cellular Biology | CelbiologieGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. | Laboratory manuals. Additional physical formats: Print version:: Human embryonic stem cells handbook.DDC classification: 616.02/774 LOC classification: QH588.S83 | H86 2012NLM classification: W1 | QU 25Online resources: Click here to access online
Contents:
Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology -- Successful Scale-Up and Quality Assessments of Human Embryonic Stem Cells for Cell Therapy: Challenges and Overview -- Human Embryonic Stem Cells Derived in Xeno-Free Conditions -- Procedures for Derivation and Characterization of Human Embryonic Stem Cells from Odense, Denmark -- Principles for Derivation of Human Embryonic Stem Cells -- Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells -- Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos -- Analysis of LINE-1 Expression in Human Pluripotent Cells -- Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan -- Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos -- Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos -- Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders -- Single-Cell Enzymatic Dissociation of hESC Lines OxF1 -- OxF4 and Culture in Feeder-Free Conditions -- Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension -- Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells -- Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs) -- Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol -- Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells -- Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells -- Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next Generation Sequencing (MeDIP-seq) -- Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis.
Summary: Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult. In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology. Written in the Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.
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Includes index.

Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology -- Successful Scale-Up and Quality Assessments of Human Embryonic Stem Cells for Cell Therapy: Challenges and Overview -- Human Embryonic Stem Cells Derived in Xeno-Free Conditions -- Procedures for Derivation and Characterization of Human Embryonic Stem Cells from Odense, Denmark -- Principles for Derivation of Human Embryonic Stem Cells -- Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells -- Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos -- Analysis of LINE-1 Expression in Human Pluripotent Cells -- Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan -- Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos -- Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos -- Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders -- Single-Cell Enzymatic Dissociation of hESC Lines OxF1 -- OxF4 and Culture in Feeder-Free Conditions -- Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension -- Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells -- Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs) -- Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol -- Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells -- Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells -- Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next Generation Sequencing (MeDIP-seq) -- Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis.

Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult. In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology. Written in the Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.

Includes bibliographical references and index.

English.

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