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Odontogenesis : methods and protocols / edited by Chrissa Kioussi.

Contributor(s): Kioussi, Chrissa [edt]Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 887.Publication details: New York : Humana Press : Springer, ©2012. Description: 1 online resource (x, 194 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781617798603; 1617798606Subject(s): Dentition | Teeth | Publication Formats | Physiological Phenomena | Dentition | Organogenesis | Embryonic and Fetal Development | Mouth | Publication Characteristics | Morphogenesis | Phenomena and Processes | Stomatognathic System | Anatomy | Growth and Development | Odontogenesis | Tooth | Laboratory Manuals | Dentition | Teeth | tanden | teeth | celbiologie | cellular biology | tandheelkunde | dentistry | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Odontogenesis.DDC classification: 612.3/11 LOC classification: QL858 | .O36 2012NLM classification: W1 | WU 25Online resources: Click here to access online
Contents:
Histological analysis of the embryonic and adult tooth / Atsushi Ohazama -- Determination of gene expression patterns by whole-mount in situ hybridization / Sergiy Kyryachenko [and others] -- Determination of gene expression patterns by in situ hybridization in sections/ Kateryna Kyrylkova [and others] -- Immunohistochemistry and detection of proliferating cells by BrdU / Sergiy Kyryachenko [and others] -- Detection of apoptosis by TUNEL assay / Kateryna Kyrylkova [and others] -- Use of siRNA in dental tissue-derived cell cultures : integrin knockdown in fibroblasts / Malgorzata M. Barczyk, Donald Gullberg, and Anne Isine Bolstad -- Organ cultures and kidney-capsule grafting of tooth germs / Keishi Otsu, Naoki Fujiwara, and Hidemitsu Harada -- Evaluation of skull and tooth morphology and mineralization using high-resolution x-ray tomography / Brian K. Bay -- Electron microscopy / Hans U. Luder and Margrit Amstad-Jossi -- Deoxyoligonucleotide microarrays for gene expression profiling in murine tooth germs / Harald Osmundsen, Anne-Marthe Jevnaker, and Maria A. Landin -- Lineage differentiation of mesenchymal stem cells from dental pulp, apical papilla, and periodontal ligament / Kentaro Akiyama [and others] -- In vivo transplantation and tooth repair / Shuhei Tsuchiya and Masaki J. Honda -- Methods to validate tooth-supporting regenerative therapies / Miguel Padial-Molina [and others] -- Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (organ germ method) / Masamitsu Oshima [and others] -- In vitro studies on odontogenic tumors / Javier Caton, Thimios A. Mitsiadis, and Peter R. Morgan -- Whole mount immunohistochemistry and in situ hybridization of larval and adultzZebra fish dental tissues / Barbara Verstraeten, Ellen Sanders, and Ann Huysseune.
Summary: Only in recent times has the possibility of growing and implanting replacement teeth, made from one's own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized. In Odontogenesis: Methods and Protocols, experts in the field examine techniques to approach this burgeoning field. This detailed volume includes chapters on the detection of "tooth development" gene expression, both at the RNA and protein level, current approaches to the manipulation of gene expression levels and subsequent analysis of tooth phenotypes, as well as chapters concerning current efforts to get living tooth implants working without waiting for a full understanding of the developmental pathways at the molecular level. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for trouble-shooting and avoiding known pitfalls. Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move forward toward the ultimate goal of getting a "bioengineered tooth" into the patient's mouth
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Histological analysis of the embryonic and adult tooth / Atsushi Ohazama -- Determination of gene expression patterns by whole-mount in situ hybridization / Sergiy Kyryachenko [and others] -- Determination of gene expression patterns by in situ hybridization in sections/ Kateryna Kyrylkova [and others] -- Immunohistochemistry and detection of proliferating cells by BrdU / Sergiy Kyryachenko [and others] -- Detection of apoptosis by TUNEL assay / Kateryna Kyrylkova [and others] -- Use of siRNA in dental tissue-derived cell cultures : integrin knockdown in fibroblasts / Malgorzata M. Barczyk, Donald Gullberg, and Anne Isine Bolstad -- Organ cultures and kidney-capsule grafting of tooth germs / Keishi Otsu, Naoki Fujiwara, and Hidemitsu Harada -- Evaluation of skull and tooth morphology and mineralization using high-resolution x-ray tomography / Brian K. Bay -- Electron microscopy / Hans U. Luder and Margrit Amstad-Jossi -- Deoxyoligonucleotide microarrays for gene expression profiling in murine tooth germs / Harald Osmundsen, Anne-Marthe Jevnaker, and Maria A. Landin -- Lineage differentiation of mesenchymal stem cells from dental pulp, apical papilla, and periodontal ligament / Kentaro Akiyama [and others] -- In vivo transplantation and tooth repair / Shuhei Tsuchiya and Masaki J. Honda -- Methods to validate tooth-supporting regenerative therapies / Miguel Padial-Molina [and others] -- Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (organ germ method) / Masamitsu Oshima [and others] -- In vitro studies on odontogenic tumors / Javier Caton, Thimios A. Mitsiadis, and Peter R. Morgan -- Whole mount immunohistochemistry and in situ hybridization of larval and adultzZebra fish dental tissues / Barbara Verstraeten, Ellen Sanders, and Ann Huysseune.

Includes bibliographical references and index.

Print version record.

Only in recent times has the possibility of growing and implanting replacement teeth, made from one's own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized. In Odontogenesis: Methods and Protocols, experts in the field examine techniques to approach this burgeoning field. This detailed volume includes chapters on the detection of "tooth development" gene expression, both at the RNA and protein level, current approaches to the manipulation of gene expression levels and subsequent analysis of tooth phenotypes, as well as chapters concerning current efforts to get living tooth implants working without waiting for a full understanding of the developmental pathways at the molecular level. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for trouble-shooting and avoiding known pitfalls. Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move forward toward the ultimate goal of getting a "bioengineered tooth" into the patient's mouth

English.

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