Receptor binding techniques / edited by Anthony P. Davenport.
Contributor(s): Davenport, Anthony PMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): volume 897.Publisher: New York, NY : London : Humana ; Springer [distributor], 2012Edition: 3rd edDescription: 1 online resource (xii, 309 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781617799099; 1617799092Subject(s): Receptor-ligand complexes -- Research -- Laboratory manuals | Ligand binding (Biochemistry) -- Laboratory manuals | Cell receptors -- Research -- Laboratory manuals | Biochemical Phenomena | Proteins | Metabolism | Diagnostic Techniques, Endocrine | Diagnostic Techniques, Radioisotope | Investigative Techniques | Laboratory Techniques and Procedures | Receptors, Cell Surface | Molecular Structure | Membrane Proteins | Diagnostic Techniques and Procedures | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Amino Acids, Peptides, and Proteins | Chemical Phenomena | Diagnosis | Chemicals and Drugs | Phenomena and Processes | Radioligand Assay | Methods | Binding Sites | Receptors, Drug | Receptors, G-Protein-Coupled | Carrier Proteins | Biology | Health & Biological Sciences | Cytology | Biophysics | Cell receptors -- Research | Ligand binding (Biochemistry) | eiwitten | proteins | moleculaire biologie | molecular biology | liganden | ligands | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Receptor binding techniques.DDC classification: 571.6 LOC classification: QH603.C43 | R39 2012Online resources: Click here to access online
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Previous edition: 2005.
Includes bibliographical references and index.
Print version record.
A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function. A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel 'biased agonists'. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding.
880-01 Receptor databases and computational websites for ligand binding / Brinda K. Rana, Philip E. Bourne, and Paul A. Insel -- How to use the IUPHAR receptor database to navigate pharmacological data / Chidochangu P. Mpamhanga [and others] -- Radioligand binding assays and their analysis / Janet J. Maguire, Rhoda E. Kuc, and Anthony P. Davenport -- Use of scintillation proximity assay to measure radioligand binding to immobilized receptors without separation of bound from free ligand / Jenny Berry, Molly Price-Jones, and Barbara Killian -- Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands / Craig J. Daly, Ingela Parmryd, and John C. McGrath -- Dissecting the pharmacology of G protein-coupled receptor signaling complexes using bimolecular fluorescence complementation / Laura E. Kilpatrick and Nicholas D. Holliday -- Live cell imaging of G protein-coupled receptors / Anke Teichmann [and others] -- Characterization of G-protein coupled receptor modulators using homogeneous cAMP assays / Daniel L. Bassoni [and others] -- Measurements of [beta]-arrestin recruitment to activated seven transmembrane receptors using enzyme complementation / Daniel L. Bassoni [and others] -- Quantitative phosphor imaging autoradiography of radioligands for positron emission tomography / Peter Johnstrom, Joseph L. Bird, and Anthony P. Davenport -- Dynamic in vivo imaging of receptors in small animals using positron emission tomography / Peter Johnstrom [and others] -- Cellular localization of receptors using antibodies visualized by light and dual labeling confocal microscopy / Anthony P. Davenport and Rhoda E. Kuc -- Detection of mRNA encoding receptors by in situ and northern hybridization / Alessandra P. Princivalle [and others].