Antiviral resistance in plants : methods and protocols / edited by John M. Watson and Ming-Bo Wang.

Contributor(s): Watson, J. M. (John Matheson) | Wang, Ming-Bo, 1962-
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 894.Publisher: New York : Humana Press : Springer, ©2012Description: 1 online resource (xi, 380 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781617798825; 1617798827; 1617798819; 9781617798818Subject(s): Plants -- Virus resistance -- Laboratory manuals | Plant immunology -- Laboratory manuals | Publication Formats | Viruses | RNA, Small Untranslated | Adaptive Immunity | Plant Physiological Phenomena | Gene Silencing | RNA, Antisense | Autoimmunity | Antisense Elements (Genetics) | Immunity | Phenomena and Processes | Organisms | RNA, Untranslated | RNA | Epigenesis, Genetic | Publication Characteristics | Nucleic Acids | Gene Expression Regulation | Immune System Phenomena | Nucleic Acids, Nucleotides, and Nucleosides | Genetic Phenomena | Chemicals and Drugs | RNA, Small Interfering | Plant Immunity | Laboratory Manuals | RNA Interference | Plant Viruses | Plant immunology | Plants -- Virus resistance | Life sciences | Medical virology | Botany | Plant diseases | Plant Sciences | Plant Pathology | Virology | planten | plants | plantenvirussen | plant viruses | ziekteresistentie | disease resistance | plant-microbe interactions | laboratoriummethoden | laboratory methods | protocollen | protocols | antivirale planten | antiviral plants | uitschakelen van genexpressie | gene silencing | Plant Viruses | Microbe-Plant Relations | Plantenvirussen | Microbe-plant relatiesGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Antiviral resistance in plants.DDC classification: 571.9/928 LOC classification: SB736 | .A58 2012Online resources: Click here to access online
Contents:
Historical overview of RNAi in plants / John A. Lindbo -- RNA silencing and antiviral defense in plants / Claire Agius, Andrew L. Eamens, Anthony A. Millar, John M. Watson, and Ming-Bo Wang -- Biological chemistry of virus-encoded suppressors of RNA silencing : an overview / Rustem T. Omarov and Herman B. Scholthof -- Virus-induced gene silencing using begomovirus satellite molecules / Xueping Zhou and Changjun Huang -- Strategies for viral cross protection in plants / Changyong Zhou and Yan Zhou -- Tobacco rattle virus (TRV)-based virus-induced gene Silencing / Shawn Bachan and Savithramma P. Dinesh-Kumar -- Virus-induced gene silencing in hexaploid wheat using barley stripe mosaic virus vectors / Steven R. Scofield and Amanda S. Brandt -- Characterization of plant virus-encoded gene silencing suppressors / Hajime Yaegashi, Masamichi Isogai, and Nobuyuki Yoshikawa -- Generation of plant small RNA cDNA libraries for high-throughput sequencing / Qian-Hao Zhu and Christopher A. Helliwell -- Modeling antiviral resistance in plants / Robert S. Anderssen and Peter M. Waterhouse -- Isolation and detection of small RNAs from plant tissues / Neil A. Smith and Andrew L. Eamens -- Isolation and analysis of small RNAs from virus-infected plants / Shaun J. Curtin, Agustín Zsögön, John M. Watson, and Peter M. Waterhouse -- Use of hairpin RNA constructs for engineering plant virus resistance / Neena Mitter and Ralf G. Dietzgen -- Artificial microRNAs for plant virus resistance / Jing Qu, Jian Ye, and Rongxiang Fang -- Isolation and biochemical analysis of plant small RNAs / H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau -- Isolation and analysis of plant RNA-dependent RNA polymerases / Julien Curaba -- Plant viroids : isolation, characterization/detection, and analysis / Robert A. Owens, Teruo Sano, and Nuria Duran-Vila -- Structural and functional analysis of CMV satellite RNAs in RNA silencing / Hanako Shimura and Chikara Masuta -- Virus-Induced Gene Silencing in Soybean / Aardra Kachroo and Said Ghabrial -- Chimeric, infectious, and stable virus transcripts to study RNA silencing in "Dark Green" Islands / Robin M. MacDiarmid -- Rolling circle amplification-mediated long hairpin RNA library construction in plants / Lei Wang and Yun-Liu Fan -- Interaction between plant viroid-induced symptoms and RNA silencing / Vicente Pallas, German Martinez, and Gustavo Gomez -- Identification of small molecule inhibitors of tomato bushy stunt virus replication / Zsuzsanna Sasvari and Peter D. Nagy -- Expression of dominant-negative mutants to study host factors affecting plant virus infections / Daniel Barajas and Peter D. Nagy.
Summary: Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.
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Includes bibliographical references and index.

Historical overview of RNAi in plants / John A. Lindbo -- RNA silencing and antiviral defense in plants / Claire Agius, Andrew L. Eamens, Anthony A. Millar, John M. Watson, and Ming-Bo Wang -- Biological chemistry of virus-encoded suppressors of RNA silencing : an overview / Rustem T. Omarov and Herman B. Scholthof -- Virus-induced gene silencing using begomovirus satellite molecules / Xueping Zhou and Changjun Huang -- Strategies for viral cross protection in plants / Changyong Zhou and Yan Zhou -- Tobacco rattle virus (TRV)-based virus-induced gene Silencing / Shawn Bachan and Savithramma P. Dinesh-Kumar -- Virus-induced gene silencing in hexaploid wheat using barley stripe mosaic virus vectors / Steven R. Scofield and Amanda S. Brandt -- Characterization of plant virus-encoded gene silencing suppressors / Hajime Yaegashi, Masamichi Isogai, and Nobuyuki Yoshikawa -- Generation of plant small RNA cDNA libraries for high-throughput sequencing / Qian-Hao Zhu and Christopher A. Helliwell -- Modeling antiviral resistance in plants / Robert S. Anderssen and Peter M. Waterhouse -- Isolation and detection of small RNAs from plant tissues / Neil A. Smith and Andrew L. Eamens -- Isolation and analysis of small RNAs from virus-infected plants / Shaun J. Curtin, Agustín Zsögön, John M. Watson, and Peter M. Waterhouse -- Use of hairpin RNA constructs for engineering plant virus resistance / Neena Mitter and Ralf G. Dietzgen -- Artificial microRNAs for plant virus resistance / Jing Qu, Jian Ye, and Rongxiang Fang -- Isolation and biochemical analysis of plant small RNAs / H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau -- Isolation and analysis of plant RNA-dependent RNA polymerases / Julien Curaba -- Plant viroids : isolation, characterization/detection, and analysis / Robert A. Owens, Teruo Sano, and Nuria Duran-Vila -- Structural and functional analysis of CMV satellite RNAs in RNA silencing / Hanako Shimura and Chikara Masuta -- Virus-Induced Gene Silencing in Soybean / Aardra Kachroo and Said Ghabrial -- Chimeric, infectious, and stable virus transcripts to study RNA silencing in "Dark Green" Islands / Robin M. MacDiarmid -- Rolling circle amplification-mediated long hairpin RNA library construction in plants / Lei Wang and Yun-Liu Fan -- Interaction between plant viroid-induced symptoms and RNA silencing / Vicente Pallas, German Martinez, and Gustavo Gomez -- Identification of small molecule inhibitors of tomato bushy stunt virus replication / Zsuzsanna Sasvari and Peter D. Nagy -- Expression of dominant-negative mutants to study host factors affecting plant virus infections / Daniel Barajas and Peter D. Nagy.

Online resource; title from PDF title page (Springer Protocols, viewed Oct. 17, 2012).

Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.

English.

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