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Antibody methods and protocols / edited by Gabriele Proetzel, Hilmar Ebersbach.

Contributor(s): Proetzel, Gabriele [edt] | Ebersbach, Hilmar [edt]Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 901. | Springer protocols (Series)Publication details: New York : Humana Press : Springer Science+Business Media, ©2012. Description: 1 online resource (x, 325 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781617799310; 1617799319Subject(s): Immunoglobulins | Antibodies | Antibody Formation | Immunoglobulins | Medicine | Immunology | Monoclonal antibodies | Biomedicine | Antibodies | biomedische wetenschappen | immunologie | antilichamen | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Antibody methods and protocols.DDC classification: 571.9/67 LOC classification: QR186.7 | .A597 2012NLM classification: W1 | QW 525Online resources: Click here to access online
Contents:
Antigen presentation for the generation of binding molecules / Hilmar Ebersbach, Gabriele Proetzel, and Chonghui Zhang -- Recombinant antibodies and in vitro selection technologies / C. Ronald Geyer, John McCafferty, Stefan Dübel, Andrew R.M. Bradbury, and Sachdev S. Sidhu -- Phage display / Konstantin Petropoulos -- Generation of human fab libraries for phage display / Christoph Rader -- Selection of human fab libraries by phage display / Christoph Rader -- Ribosome Display / George Thom and Maria Groves -- Hybridoma technology for the generation of monoclonal antibodies / Chonghui Zhang -- Application of transgenic mice for therapeutic antibody discovery / E-Chiang Lee and Michael Owen -- Production of human or humanized antibodies in mice / Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné -- Immortalization of human B cells : analysis of B cell repertoire and production of human monoclonal antibodies / Elisabetta Traggiai -- Kinetic screening in the antibody development process / Michael Schräml and Matthias Biehl -- Temperature-dependent antibody kinetics as a tool in antibody lead selection / Michael Schräml and Leopold von Proff -- Determination of antibody glycosylation by mass spectrometry / Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll -- Cloning, expression, and purification of monoclonal antibodies in scFv-Fc format / Jiahui Yang and Christoph Rader -- PEGylation of antibody fragments for half-life extension / Simona Jevševar, Mateja Kusterle, and Maja Kenig -- Bispecific antibody derivatives based on full-length IgG formats / Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann -- Generation of fluorescent IgG fusion proteins in mammalian Cells / Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann -- Methods to engineer and identify IgG1 variants with improved FcRn binding or effector function / Robert F. Kelley and Y. Gloria Meng -- Class-specific effector functions of therapeutic antibodies / Virginie Pascal, Brice Laffleur, and Michel Cogné.
Summary: The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies. Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments. The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.
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Antigen presentation for the generation of binding molecules / Hilmar Ebersbach, Gabriele Proetzel, and Chonghui Zhang -- Recombinant antibodies and in vitro selection technologies / C. Ronald Geyer, John McCafferty, Stefan Dübel, Andrew R.M. Bradbury, and Sachdev S. Sidhu -- Phage display / Konstantin Petropoulos -- Generation of human fab libraries for phage display / Christoph Rader -- Selection of human fab libraries by phage display / Christoph Rader -- Ribosome Display / George Thom and Maria Groves -- Hybridoma technology for the generation of monoclonal antibodies / Chonghui Zhang -- Application of transgenic mice for therapeutic antibody discovery / E-Chiang Lee and Michael Owen -- Production of human or humanized antibodies in mice / Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné -- Immortalization of human B cells : analysis of B cell repertoire and production of human monoclonal antibodies / Elisabetta Traggiai -- Kinetic screening in the antibody development process / Michael Schräml and Matthias Biehl -- Temperature-dependent antibody kinetics as a tool in antibody lead selection / Michael Schräml and Leopold von Proff -- Determination of antibody glycosylation by mass spectrometry / Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll -- Cloning, expression, and purification of monoclonal antibodies in scFv-Fc format / Jiahui Yang and Christoph Rader -- PEGylation of antibody fragments for half-life extension / Simona Jevševar, Mateja Kusterle, and Maja Kenig -- Bispecific antibody derivatives based on full-length IgG formats / Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann -- Generation of fluorescent IgG fusion proteins in mammalian Cells / Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann -- Methods to engineer and identify IgG1 variants with improved FcRn binding or effector function / Robert F. Kelley and Y. Gloria Meng -- Class-specific effector functions of therapeutic antibodies / Virginie Pascal, Brice Laffleur, and Michel Cogné.

Includes bibliographical references and index.

Print version record.

The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies. Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments. The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.

English.

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