Biolistic DNA delivery : methods and protocols / edited by Stephan Sudowe, Angelika B. Reske-Kunz.Material type: TextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 940.Publication details: New York : Humana Press : Springer, ©2013. Description: 1 online resource (xiv, 415 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781627031103; 1627031103Subject(s): Medicine | Human genetics | Genetic engineering | Injections, Jet | Gene Transfer Techniques | Genetic Techniques | Injections, Subcutaneous | Investigative Techniques | Injections | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Drug Administration Routes | Drug Therapy | Therapeutics | Biolistics | Genetic engineering | Human genetics | Medicine | biomedische wetenschappen | biomedicine | genetische modificatie | genetic engineering | humane genetica | human genetics | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Biolistic DNA delivery.DDC classification: 611.01816 LOC classification: RB155-155.8NLM classification: W1 | WB 354Online resources: Click here to access online
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Comparison between Agrobacterium-mediated and direct gene transfer using the gene gun / Caixia Gao and Klaus K. Nielsen -- Transient gene expression in epidermal cells of plant leaves by biolistic DNA delivery / Shoko Ueki [and others] -- Transformation of nuclear DNA in meristematic and embryogenic tissues / Mulpuri Sujatha and K.B.R.S. Visarada -- Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (handygun) / Anssi L. Vuorinen [and others] -- Handgun-mediated inoculation of plants with viral pathogens for mechanistic studies / Victor Gaba, Moshe Lapidot, and Amit Gal-On -- Biolistics-based gene silencing in plants using a modified particle inflow gun / Kevin M. Davies [and others] -- Biolistic transformation of Caenorhabditis elegans / Meltem Isik and Eugene Berezikov -- Improved vectors for selection of transgenic Caenorhabditis elegans / Annabel A. Ferguson [and others] -- Biolistic transformation of Brugia Malayi / Tarig B. Higazi and Thomas R. Unnasch -- Biolistic transfection of human embryonic kidney (HEK) 293 cells / Xiongwei Li [and others] -- Biolistic transfection of tumor tissue samples / Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang -- Biolistic transfection of freshly isolated adult ventricular myocytes / David F. Steele, Ying Dou, and David Fedida -- Biolistic transfection of neurons in organotypic brain slices / John A. O'Brien and Sarah C.R. Lummis -- Biolistic DNA delivery to mice with the low pressure gene gun / Meng-Chi Yen and Ming-Derg Lai -- Chemokine overexpression in the skin by biolistic DNA delivery / Ahmad Jalili -- Enhancement of gene gun-induced vaccine-specific cytotoxic T-cell response by administration of chemotherapeutic drugs / Steve Pascolo -- Dendritic cell-specific biolistic transfection using the Fascin gene promoter / Yvonne Hohn, Stephan Sudowe, and Angelika B. Reske-Kunz -- Particle-mediated administration of plasmid DNA on corneas of BALB/c mice / Dirk Bauer [and others] -- Optimizing particle-mediated epidermal delivery of an influenza DNA vaccine in ferrets / Eric J. Yager [and others] -- Methods for monitoring gene gun-induced HBV and HCV-specific immune responses in mouse models / Gustaf Ahlen, Matti Sallberg, and Lars Frelin -- Gene gun immunization to combat malaria / Elke S. Bergmann-Leitner and Wolfgang W. Leitner -- Identification of T cell epitopes of Mycobacterium tuberculosis with biolistic DNA vaccination / Toshi Nagata and Yukio Koide -- Biolistic DNA vaccination against trypanosoma infection / Marianne Bryan, Siobhan Guyach, and Karen A. Norris -- Biolistic DNA vaccination against melanoma / Julia Steitz and Thomas Tuting -- Biolistic DNA vaccination against cervical cancer / Michal Smahel -- Efficiency of biolistic DNA vaccination in experimental type I allergy / Verena Raker [and others] -- Safety assessment of biolistic DNA vaccination / Barbara Langer [and others] -- DiOlistics: delivery of fluorescent dyes into cells / Nyssa Sherazee and Veronica A. Alvarez -- Protein antigen delivery by gene gun-mediated epidermal antigen incorporation (EAI) / Sandra Scheiblhofer [and others].
Includes bibliographical references and index.
Print version record.
Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.