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Immune homeostasis : methods and protocols / edited by Andrew L. Snow, Michael J. Lenardo.

Contributor(s): Snow, Andrew L | Lenardo, MichaelMaterial type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 979.Publication details: New York : Humana Press : Springer, ©2013. Description: 1 online resource (xi, 215 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781627032902; 1627032908; 1627032894; 9781627032896Subject(s): Immunology -- Laboratory manuals | Immune System | Homeostasis | Immunology | biomedische wetenschappen | biomedicine | immunologie | immunology | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Immune homeostasis.DDC classification: 571.9/6 LOC classification: QR183 | .I46 2013NLM classification: W1 | QW 540Online resources: Click here to access online
Contents:
Quantitating lymphocyte programmed cell death in vitro using simple kill assays / Lixin Zheng -- Fluorescence-activated cell sorting-based quantitation of T cell receptor restimulation-induced cell death in activated, primary human T cells / Gil Katz and Andrew L. Snow -- Evaluation of IL-2-withdrawal-induced apoptosis in human T lymphocytes / Joao Bosco Oliveira -- Determination of apoptosis sensitivity in specific T cell subsets from human peripheral blood by utilizing a multiparameter fluorescence-activated cell sorting-based technique / Bernice Lo and Madhu Ramaswamy -- Visualization of fas-mediated death-inducing signaling complex formation by immunoprecipitation / Nelia Cordeiro and Nicolas Bidere -- Analyses of programmed cell death in dendritic cells / Min Chen, Lily Huang, and Jin Wang -- Detection of necrosis by release of lactate dehydrogenase Activity / Francis Ka-Ming Chan, Kenta Moriwaki, and Maria Jose De Rosa -- Assessment of CD4+ and CD8+ T cell responses using MHC class I and II tetramers / Sema Kurtulus and David Hildeman -- Homeostatic proliferation of mature T cells / Christopher E. Martin [and others] -- Quantitating lymphocyte homeostasis in vivo in humans using stable isotope tracers / Liset Westera [and others] -- Real-time quantitative (RQ- )PCR approach to quantify the contribution of proliferation to B lymphocyte homeostasis / Menno C. van Zelm [and others] -- Molecular measurement of T cell receptor excision circles / Heather E. Lynch and Gregory D. Sempowski -- Isolation of RNA and the synthesis and amplification of cDNA from antigen-specific T cells for genome-wide expression analysis / R. Anthony Barnitz [and others] -- Designs for massively parallel sequencing approaches to identify causal mutations in human immune disorders / Yu Zhang and Helen C. Su -- Flow cytometric measurement of SLAM-associated protein and x-linked inhibitor of apoptosis / Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich -- In vitro suppression assay for functional assessment of human regulatory T cells / Dat Q. Tran.
Summary: Many questions remain unresolved as researchers continue to characterize and define the nature of normal immune homeostasis and determine how these processes are dysregulated in immunodeficiency, as well as in autoimmune and lymphoproliferative disorders. Immune Homeostasis: Methods and Protocols focuses on experimental techniques for measuring and analyzing immune cell dynamics, with a particular emphasis on examining lymphocyte programmed cell death in different contexts. With contributions by leading experts in the field, the collection examines detailed protocols for studying various pathways of apoptosis and necrosis in different types of hematopoietic cells, both in vitro and in vivo, methods for studying the maintenance of lymphocyte populations in the steady-state or following infectious challenges in both mice and humans, and technical insights into state-of-the-art genomics tools, among other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Valuable and easy to use, Immune Homeostasis: Methods and Protocols serves as an important toolkit for basic and clinical scientists interested in examining various aspects of immune homeostasis in both normal and disease-related contexts in order to further study the dynamic processes that contribute to homeostasis of the immune system.
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Includes bibliographical references and index.

Quantitating lymphocyte programmed cell death in vitro using simple kill assays / Lixin Zheng -- Fluorescence-activated cell sorting-based quantitation of T cell receptor restimulation-induced cell death in activated, primary human T cells / Gil Katz and Andrew L. Snow -- Evaluation of IL-2-withdrawal-induced apoptosis in human T lymphocytes / Joao Bosco Oliveira -- Determination of apoptosis sensitivity in specific T cell subsets from human peripheral blood by utilizing a multiparameter fluorescence-activated cell sorting-based technique / Bernice Lo and Madhu Ramaswamy -- Visualization of fas-mediated death-inducing signaling complex formation by immunoprecipitation / Nelia Cordeiro and Nicolas Bidere -- Analyses of programmed cell death in dendritic cells / Min Chen, Lily Huang, and Jin Wang -- Detection of necrosis by release of lactate dehydrogenase Activity / Francis Ka-Ming Chan, Kenta Moriwaki, and Maria Jose De Rosa -- Assessment of CD4+ and CD8+ T cell responses using MHC class I and II tetramers / Sema Kurtulus and David Hildeman -- Homeostatic proliferation of mature T cells / Christopher E. Martin [and others] -- Quantitating lymphocyte homeostasis in vivo in humans using stable isotope tracers / Liset Westera [and others] -- Real-time quantitative (RQ- )PCR approach to quantify the contribution of proliferation to B lymphocyte homeostasis / Menno C. van Zelm [and others] -- Molecular measurement of T cell receptor excision circles / Heather E. Lynch and Gregory D. Sempowski -- Isolation of RNA and the synthesis and amplification of cDNA from antigen-specific T cells for genome-wide expression analysis / R. Anthony Barnitz [and others] -- Designs for massively parallel sequencing approaches to identify causal mutations in human immune disorders / Yu Zhang and Helen C. Su -- Flow cytometric measurement of SLAM-associated protein and x-linked inhibitor of apoptosis / Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich -- In vitro suppression assay for functional assessment of human regulatory T cells / Dat Q. Tran.

Print version record.

Many questions remain unresolved as researchers continue to characterize and define the nature of normal immune homeostasis and determine how these processes are dysregulated in immunodeficiency, as well as in autoimmune and lymphoproliferative disorders. Immune Homeostasis: Methods and Protocols focuses on experimental techniques for measuring and analyzing immune cell dynamics, with a particular emphasis on examining lymphocyte programmed cell death in different contexts. With contributions by leading experts in the field, the collection examines detailed protocols for studying various pathways of apoptosis and necrosis in different types of hematopoietic cells, both in vitro and in vivo, methods for studying the maintenance of lymphocyte populations in the steady-state or following infectious challenges in both mice and humans, and technical insights into state-of-the-art genomics tools, among other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Valuable and easy to use, Immune Homeostasis: Methods and Protocols serves as an important toolkit for basic and clinical scientists interested in examining various aspects of immune homeostasis in both normal and disease-related contexts in order to further study the dynamic processes that contribute to homeostasis of the immune system.

English.

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