Stem cells and aging : methods and protocols / edited by Kursad Turksen.
Contributor(s): Turksen, KursadMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 976.Publisher: New York : Humana Press : Springer, ©2013Description: 1 online resource (xi, 179 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781627033176; 1627033173Subject(s): Stem cells -- Laboratory manuals | Aging -- Molecular aspects -- Laboratory manuals | Stem Cells | Biology | Health & Biological Sciences | Cytology | Aging -- Molecular aspects | Stem cells | celbiologie | cellular biology | stamcellen | stem cells | geneeskunde | medicine | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Stem cells and aging.DDC classification: 616.02/774 LOC classification: QH588.S83 | S74 2013Online resources: Click here to access online
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Includes bibliographical references and index.
Comprehensive hematopoietic stem cell isolation methods / Kyle Rector, Yi Liu, and Gary Van Zant -- Serial transplantation of bone marrow to test self-renewal capacity of hematopoietic stem cells in vivo / Charusheila Ramkumar, Rachel M. Gerstein, and Hong Zhang -- Mouse hematopoietic stem cell transplantation / Hui Cheng, Paulina H. Liang, and Tao Cheng -- Isolation, characterization, and transplantation of adult liver progenitor cells / Mladen I. Yovchev, Mariana D. Dabeva, and Michael Oertel -- Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces / Mitra Lavasani [and others] -- Human myoblasts from skeletal muscle biopsies : in vitro culture preparations for morphological and cytochemical analyses at light and electron microscopy / Manuela Malatesta [and others] -- Cardiac stem cell senescence / Daniela Cesselli [and others] -- Isolation of mesenchymal stem cells from human bone and long-term cultivation under physiologic oxygen conditions / Sebastian Klepsch [and others] -- Methods for assessing effects of Wnt/b-catenin signaling in senescence of mesenchymal stem cells / Hai-jie Wang and Yu-zhen Tan -- Intra-femoral injection of human mesenchymal stem cells / Sindhu T. Mohanty and Ilaria Bellantuono -- Tracking of replicative senescence in mesenchymal stem cells by colony-forming unit frequency / Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner -- Chromatin immunoprecipitation coupled by quantitative real-time PCR as a tool for analyzing epigenetic regulation of stem cell aging / Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang -- Quantitative fluorescence in situ hybridization on paraffin embedded tissue / Mario Ricciardi, Mauro Krampera, and Marco Chilosi.
Print version record.
Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome. Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population.
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