Jasmonate signaling : methods and protocols / edited by Alain Goossens, Laurens Pauwels.
Contributor(s): Goossens, Alain | Pauwels, LaurensMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): 1011.Publisher: New York : Humana Press, ©2013Description: 1 online resourceContent type: text Media type: computer Carrier type: online resourceISBN: 9781627034142; 1627034145; 1627034137; 9781627034135Subject(s): Plant cellular signal transduction -- Laboratory manuals | Growth (Plants) -- Regulation -- Laboratory manuals | Botanical chemistry -- Laboratory manuals | Chemistry | Plant Growth Regulators | Plant Development | Signal Transduction | Botanical chemistry | Growth (Plants) -- Regulation | Plant cellular signal transduction | Life sciences | Biochemistry | Botany | Plant Sciences | Plant Biochemistry | jasmonzuur | jasmonic acid | planten | plants | plantensamenstelling | plant composition | biochemie | plantkunde | signaaltransductie | signal transduction | bioinformatics | laboratoriummethoden | laboratory methods | protocollen | protocols | Plant Composition | Biological Techniques | Plantensamenstelling | Biologische techniekenGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Jasmonate signaling.DDC classification: 571.7/42 LOC classification: QK725 | .J37 2013Online resources: Click here to access online
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Includes bibliographical references and index.
Phenotyping jasmonate regulation of senescence / Martin A. Seltmann and Susanne Berger -- Characterizing jasmonate regulation of male fertility in Arabidopsis / Bryan Thines, Ajin Mandaokar, and John Browse -- Phenotyping jasmonate regulation of root growth / Fabian Kellermeier and Anna Amtmann -- Bioassays for assessing jasmonate-dependent defenses triggered by pathogens, herbivorous insects, or beneficial Rhizobacteria / Saskia C.M. Van Wees [and others] -- Elicitation of jasmonate-mediated defense responses by mechanical wounding and insect herbivory / Marco Herde, Abraham J.K. Koo, and Gregg A. Howe -- Pseudomonas syringae infection assays in Arabidopsis / Jian Yao, John Withers, and Sheng Yang He -- Jasmonate signaling in the field, part I : elicited changes in jasmonate pools of transgenic Nicotiana attenuata / Emmanuel Gaquerel [and others] -- Jasmonate Signaling in the field, part II : insect-guided characterization of genetic variations in jasmonate-dependent defenses of transgenic and natural Nicotiana attenuata populations / Emmanuel Gaquerel [and others] -- Profiling of jasmonic acid-related metabolites and hormones in wounded leaves / Yusuke Jikumaru -- Non-targeted approach for extended liquid chromatography-mass spectrometry profiling of free and esterified jasmonates after wounding / Gaëtan Glauser and Jean-Luc Wolfender -- Cell-specific detection of jasmonates by means of an immunocytological approach / Bettina Hause, Kati Mielke, and Susanne Forner -- Jasmonic acid-amino acid conjugation enzyme assays / Martha L. Rowe and Paul E. Staswick -- Pull-down analysis of interactions among jasmonic acid core signaling proteins / Sandra Fonseca and Roberto Solano -- Yeast two-hybrid analysis of jasmonate signaling proteins / Amparo Pérez Cuéllar [and others] -- Modified bimolecular fluorescence complementation assay to study the inhibition of transcription complex formation by JAZ proteins / Tiancong Qi, Susheng Song, and Daoxin Xie -- Agroinfiltration of Nicotiana benthamiana leaves for co-localization of regulatory proteins involved in jasmonate signaling / Volkan Çevik and Kemal Kazan -- Electrophoretic mobility shift assay for the analysis of interactions of jasmonic acid-responsive transcription factors with DNA / Johan Memelink -- Transient expression assays in tobacco protoplasts / Robin Vanden Bossche [and others] -- Functional analysis of jasmonic acid-responsive secondary metabolite transporters / Nobukazu Shitan, Akifumi Sugiyama, and Kazufumi Yazaki -- Expression analysis of jasmonate-responsive lectins in plants / Nausicaä Lannoo and Els J.M. Van Damme -- Profiling the jasmonic acid responses by nuclear magnetic resonance-based metabolomics / Hye Kyong Kim, Young Hae Choi, and Robert Verpoorte -- Metabolite profiling of plant tissues by liquid chromatography fourier transform ion cyclotron resonance mass spectrometry / Jacob Pollier and Alain Goossens -- cDNA-AFLP-based transcript profiling for genome-wide expression analysis of jasmonate-treated plants and plant cultures / Janine Colling [and others] -- Analysis of RNA-seq data with TopHat and cufflinks for genome-wide expression analysis of jasmonate-treated plants and plant cultures / Jacob Pollier, Stephane Rombauts, and Alain Goossens -- Transcriptome coexpression analysis using ATTED-II for integrated transcriptomic/metabolomic analysis / Keiko Yonekura-Sakakibara and Kazuki Saito -- Guide to CORNET for the construction of coexpression and protein-protein interaction networks / Stefanie De Bodt and Dirk Inzé.
It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.