Neural development : methods and protocols / edited by Renping Zhou, Lin Mei.

Contributor(s): Zhou, Renping [editor.] | Mei, Lin, 1959- [editor.]
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1018.Publisher: New York : Humana Press : Springer, [2013]Copyright date: ©2013Description: 1 online resource (xiii, 347 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781627034449; 1627034447Subject(s): Neurons -- Growth | Developmental neurobiology | Developmental neurobiology | Neurons -- Growth | Zoology | Biology | Health & Biological Sciences | Biophysics | Animal Anatomy & Embryology | Nervous System -- growth & development | Nervous SystemGenre/Form: Electronic books. Additional physical formats: Print version:: Neural development.DDC classification: 573.8/536 LOC classification: QP363.5Online resources: Click here to access online
Contents:
Isolation and maintenance of cortical neural progenitor cells in vitro / Mi-Yeon Kim, Byoung-San Moon, and Kang-Yell Choi -- Neural induction with a dopaminergic phenotype from human pluripotent stem cells through a feeder-free floating aggregation culture / Asuka Morizane, Daisuke Doi, and Jun Takahashi -- Nucleoside analog labeling of neural stem cells and their progeny / Erik Michael DeBoer and Mladen-Roko Rasin -- Culture of dissociated hippocampal neurons / Yun Peng, Wen-Cheng Xiong, and Lin Mei -- Culturing mouse cerebellar granule neurons / Tharakeswari Selvakumar and Daniel L. Kilpatrick -- Preparation of primary cultured dopaminergic neurons from mouse nrain / Won-Seok Choi, Hyung-Wook Kim, and Zhengui Xia -- Culturing astrocytes from postnatal rats / Yangyang Huang and Cheryl F. Dreyfus -- Culturing oligodendrocyte lineage cells from neonatal rats / Clifton G. Fulmer and Cheryl F. Dreyfus -- Isolation and culture of schwann sells / Yanmei Tao -- DNA transfection : calcium phosphate method / Munjin Kwon and Bonnie L. Firestein -- Gene-gun approach for transfection and labeling of cells in brain slices / Anna Dunaevsky -- Lentiviral vector production, titration, and transduction of primary neurons / Baojin Ding and Daniel L. Kilpatrick -- In ovo electroporation in embryonic chick spinal cords / Hui Wang and Michael P. Matise -- Gene transfer in developing chick embryos : in ovo electroporation / Emma K. Farley -- In utero electroporation in mice / Chunlei Wang and Lin Mei -- General introduction to in situ hybridization protocol using nonradioactively labeled probes to detect mRNAs on tissue sections / Daehoon Lee, Shan Xiong, and Wen-Cheng Xiong -- Immunofluorescence staining with frozen mouse or chick embryonic tissue sections / Hui Wang and Michael P. Matise -- [beta]-galactosidase staining of LacZ fusion proteins in whole tissue preparations / Margaret A. Cooper and Renping Zhou -- Chromatin immunoprecipitation assay of brain tissues using percoll gradient-purified nuclei / Baojin Ding and Daniel L. Kilpatrick -- In vivo dual luciferase reporter assay with chick neural tube in ovo electroporation system / Hui Wang and Michael P. Matise -- Growth cone collapse assay / Xin Yue, Alexander I. Son, and Renping Zhou -- Stripe assay : studying growth preference and axon guidance on binary choice substrates in vitro / Markus Weschenfelder [and others] -- Microcontact printing of substrate-bound protein patterns for cell and tissue culture / Martin Fritz and Martin Bastmeyer -- Semiautomated analysis of dendrite morphology in cell culture / Eric S. Sweet [and others] -- Monitoring synaptic plasticity by imaging AMPA receptor content and dynamics on dendritic spines / Hiroshi Makino and Bo Li -- Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms / Haitao Wu and Lin Mei -- Routine histology techniques for the developing and adult central nervous system / W. Geoffrey McAuliffe -- Cryosectioning / Alexander I. Son, Katie Sokolowski, and Renping Zhou -- Golgi-cox method / Gitanjali Das, Kenneth Reuhl, and Renping Zhou -- Neuroanatomical tract-tracing using biotinylated dextran amine / Nikolai E. Lazarov -- Retrograde tracing technique for neonatal animals / Kengo Funakoshi, Akira Yoshikawa, and Yoshitoshi Atobe.
Summary: Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Protocols, experts in the field contribute commonly used protocols to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, protocols for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and accessible, Neural Development: Methods and Protocols provides key guidance for students and postdoctoral fellows new to developmental neurobiology.
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Includes bibliographical references and index.

Print version record.

Isolation and maintenance of cortical neural progenitor cells in vitro / Mi-Yeon Kim, Byoung-San Moon, and Kang-Yell Choi -- Neural induction with a dopaminergic phenotype from human pluripotent stem cells through a feeder-free floating aggregation culture / Asuka Morizane, Daisuke Doi, and Jun Takahashi -- Nucleoside analog labeling of neural stem cells and their progeny / Erik Michael DeBoer and Mladen-Roko Rasin -- Culture of dissociated hippocampal neurons / Yun Peng, Wen-Cheng Xiong, and Lin Mei -- Culturing mouse cerebellar granule neurons / Tharakeswari Selvakumar and Daniel L. Kilpatrick -- Preparation of primary cultured dopaminergic neurons from mouse nrain / Won-Seok Choi, Hyung-Wook Kim, and Zhengui Xia -- Culturing astrocytes from postnatal rats / Yangyang Huang and Cheryl F. Dreyfus -- Culturing oligodendrocyte lineage cells from neonatal rats / Clifton G. Fulmer and Cheryl F. Dreyfus -- Isolation and culture of schwann sells / Yanmei Tao -- DNA transfection : calcium phosphate method / Munjin Kwon and Bonnie L. Firestein -- Gene-gun approach for transfection and labeling of cells in brain slices / Anna Dunaevsky -- Lentiviral vector production, titration, and transduction of primary neurons / Baojin Ding and Daniel L. Kilpatrick -- In ovo electroporation in embryonic chick spinal cords / Hui Wang and Michael P. Matise -- Gene transfer in developing chick embryos : in ovo electroporation / Emma K. Farley -- In utero electroporation in mice / Chunlei Wang and Lin Mei -- General introduction to in situ hybridization protocol using nonradioactively labeled probes to detect mRNAs on tissue sections / Daehoon Lee, Shan Xiong, and Wen-Cheng Xiong -- Immunofluorescence staining with frozen mouse or chick embryonic tissue sections / Hui Wang and Michael P. Matise -- [beta]-galactosidase staining of LacZ fusion proteins in whole tissue preparations / Margaret A. Cooper and Renping Zhou -- Chromatin immunoprecipitation assay of brain tissues using percoll gradient-purified nuclei / Baojin Ding and Daniel L. Kilpatrick -- In vivo dual luciferase reporter assay with chick neural tube in ovo electroporation system / Hui Wang and Michael P. Matise -- Growth cone collapse assay / Xin Yue, Alexander I. Son, and Renping Zhou -- Stripe assay : studying growth preference and axon guidance on binary choice substrates in vitro / Markus Weschenfelder [and others] -- Microcontact printing of substrate-bound protein patterns for cell and tissue culture / Martin Fritz and Martin Bastmeyer -- Semiautomated analysis of dendrite morphology in cell culture / Eric S. Sweet [and others] -- Monitoring synaptic plasticity by imaging AMPA receptor content and dynamics on dendritic spines / Hiroshi Makino and Bo Li -- Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms / Haitao Wu and Lin Mei -- Routine histology techniques for the developing and adult central nervous system / W. Geoffrey McAuliffe -- Cryosectioning / Alexander I. Son, Katie Sokolowski, and Renping Zhou -- Golgi-cox method / Gitanjali Das, Kenneth Reuhl, and Renping Zhou -- Neuroanatomical tract-tracing using biotinylated dextran amine / Nikolai E. Lazarov -- Retrograde tracing technique for neonatal animals / Kengo Funakoshi, Akira Yoshikawa, and Yoshitoshi Atobe.

Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Protocols, experts in the field contribute commonly used protocols to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, protocols for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and accessible, Neural Development: Methods and Protocols provides key guidance for students and postdoctoral fellows new to developmental neurobiology.

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