Tandem repeats in genes, proteins, and disease : methods and protocols / edited by Danny M. Hatters, Anthony J. Hannan.

Contributor(s): Hatters, Danny M | Hannan, Anthony J, 1969-
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1017.Publisher: New York : Humana Press, ©2013Description: 1 online resource (xi, 258 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781627034388; 1627034382; 1627034374; 9781627034371Subject(s): Nucleotides -- Laboratory manuals | Genetics -- Laboratory manuals | Publication Formats | Repetitive Sequences, Nucleic Acid | Biology | Genome Components | Pathologic Processes | Phenomena and Processes | Genome | Pathological Conditions, Signs and Symptoms | Base Sequence | Publication Characteristics | Biological Science Disciplines | Natural Science Disciplines | Genetic Structures | Molecular Structure | Disease | Disciplines and Occupations | Biochemical Phenomena | Chemical Phenomena | Genetic Phenomena | Tandem Repeat Sequences | Genetics | Laboratory Manuals | Genetics | Nucleotides | Biology | Human Anatomy & Physiology | Health & Biological Sciences | Biophysics | Animal Biochemistry | Medicine | Human genetics | Medical genetics | Biomedicine | Gene Function | biomedische wetenschappen | moleculaire genetica | molecular genetics | humane genetica | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Tandem repeats in genes, proteins, and disease.DDC classification: 572.8/5 LOC classification: QP625.N89 | .T36 2013Online resources: Click here to access online
Contents:
Longitudinal imaging and analysis of neurons expressing polyglutamine-expanded proteins / Andrey S. Tsvetkov, D. Michael Ando, and Steven Finkbeiner -- Atomic force microscopy assays for evaluating polyglutamine aggregation in solution and on surfaces / Kathleen A. Burke and Justin Legleiter -- Morphometric analysis of Huntington's disease neurodegeneration in Drosophila / Wan Song [and others] -- Size analysis of polyglutamine protein aggregates using fluorescence detection in an analytical ultracentrifuge / Saskia Polling, Danny M. Hatters, and Yee-Foong Mok -- Method for the incremental expansion of polyglutamine repeats in recombinant proteins / Amy L. Robertson and Stephen P. Bottomley -- Pulse shape analysis (PulSA) to track protein translocalization in cells by flow cytometry : applications for polyglutamine aggregation / Yasmin M. Ramdzan, Rebecca Wood, and Danny M. Hatters -- Characterizing social behavior in genetically targeted mouse models of brain disorders / Emma L. Burrows and Anthony J. Hannan -- PCR amplification and sequence analysis of GC-rich sequences : aristaless-related homeobox example / May H. Tan, Jozef Gécz, and Cheryl Shoubridge -- Challenges of "Sticky" co-immunoprecipitation : polyalanine tract protein-protein interactions / T.R. Mattiske [and others] -- Molecular pathology of polyalanine expansion disorders : new perspectives from mouse models / James N. Hughes and Paul Q. Thomas -- Yeast as a platform to explore polyglutamine toxicity and aggregation / Martin L. Duennwald -- Immuno-based detection assays to quantify distinct mutant huntingtin conformations in biological samples / Gregor P. Lotz and Andreas Weiss -- Modeling and analysis of repeat RNA toxicity in Drosophila / S.E. Samaraweera [and others] -- Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis / Mats Holmberg and Ellen A.A. Nollen -- Kinetic analysis of aggregation data / Regina M. Murphy -- Bioinformatics method for identifying Q/N-rich prion-like domains in proteins / Eric D. Ross [and others] -- Detecting soluble polyQ oligomers and investigating their impact on living cells using split-GFP / Patrick Lajoie and Erik Lee Snapp -- Cell biological approaches to investigate polyglutamine-expanded AR metabolism / Lori J. Cooper and Diane E. Merry.
Summary: The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.
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Longitudinal imaging and analysis of neurons expressing polyglutamine-expanded proteins / Andrey S. Tsvetkov, D. Michael Ando, and Steven Finkbeiner -- Atomic force microscopy assays for evaluating polyglutamine aggregation in solution and on surfaces / Kathleen A. Burke and Justin Legleiter -- Morphometric analysis of Huntington's disease neurodegeneration in Drosophila / Wan Song [and others] -- Size analysis of polyglutamine protein aggregates using fluorescence detection in an analytical ultracentrifuge / Saskia Polling, Danny M. Hatters, and Yee-Foong Mok -- Method for the incremental expansion of polyglutamine repeats in recombinant proteins / Amy L. Robertson and Stephen P. Bottomley -- Pulse shape analysis (PulSA) to track protein translocalization in cells by flow cytometry : applications for polyglutamine aggregation / Yasmin M. Ramdzan, Rebecca Wood, and Danny M. Hatters -- Characterizing social behavior in genetically targeted mouse models of brain disorders / Emma L. Burrows and Anthony J. Hannan -- PCR amplification and sequence analysis of GC-rich sequences : aristaless-related homeobox example / May H. Tan, Jozef Gécz, and Cheryl Shoubridge -- Challenges of "Sticky" co-immunoprecipitation : polyalanine tract protein-protein interactions / T.R. Mattiske [and others] -- Molecular pathology of polyalanine expansion disorders : new perspectives from mouse models / James N. Hughes and Paul Q. Thomas -- Yeast as a platform to explore polyglutamine toxicity and aggregation / Martin L. Duennwald -- Immuno-based detection assays to quantify distinct mutant huntingtin conformations in biological samples / Gregor P. Lotz and Andreas Weiss -- Modeling and analysis of repeat RNA toxicity in Drosophila / S.E. Samaraweera [and others] -- Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis / Mats Holmberg and Ellen A.A. Nollen -- Kinetic analysis of aggregation data / Regina M. Murphy -- Bioinformatics method for identifying Q/N-rich prion-like domains in proteins / Eric D. Ross [and others] -- Detecting soluble polyQ oligomers and investigating their impact on living cells using split-GFP / Patrick Lajoie and Erik Lee Snapp -- Cell biological approaches to investigate polyglutamine-expanded AR metabolism / Lori J. Cooper and Diane E. Merry.

Includes bibliographical references and index.

The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.

English.

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