The Myc gene : methods and protocols / edited by Laura Soucek, Vall d'Hebron Institute of Oncology (VHIO), Barcelona, Spain, Nicole M. Sodir, Department of Biochemistry, University of Cambridge, Cambridge, UK.Material type: TextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1012.Publisher: New York : Humana Press, 2013Description: 1 online resource (xi, 282 pages) : illustrations (some in color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781627034296; 1627034293; 1627034285; 9781627034289Subject(s): Myc oncogenes -- Laboratory manuals | Publication Formats | Proto-Oncogenes | Neoplastic Processes | Pathologic Processes | Oncogenes | Publication Characteristics | Neoplasms | Genes, Neoplasm | Pathological Conditions, Signs and Symptoms | Disease | Genes | Genome Components | Genome | Genetic Structures | Genetic Phenomena | Phenomena and Processes | Laboratory Manuals | Cell Transformation, Neoplastic | Genes, myc | Medicine | Health & Biological Sciences | Metabolic & Nutritional Diseases | Myc oncogenes | moleculaire biologie | molecular biology | celbiologie | cellular biology | genetica | genetics | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Myc gene.DDC classification: 616.994071 LOC classification: RC268.44.M93NLM classification: W1 | QZ 25Online resources: Click here to access online
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Includes bibliographical references and index.
The Myc world within Reach -- Methods for the Expression, Purification, Preparation and Biophysical Characterization of Constructs of the c-Myc and Max b-HLH-LZs -- Analyzing Myc in Cell Transformation and Evolution -- Identifying Myc Interactors -- Detection of c-Myc Protein-Protein Interactions And Phosphorylation Status by Immunoprecipitation -- An Efficient Way of Studying Protein Protein Interactions Involving HIF-, c-Myc, and Sp1 -- Methods for Determining Myc-induced Apoptosis -- Methods to Study Myc-regulated Cellular Senescence -- Chromatin Immunoprecipitation Assays for Myc and N-Myc -- Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene Repression -- Genome-wide Analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing -- A High Throughput siRNA Screening Platform to Identify MYC Synthetic Lethal Genes as Candidate Therapeutic Targets -- Investigating Myc-dependent Translational Regulation in Normal and Cancer Cells -- Studying Myc s Role in Metabolism Regulation -- Generation of a Tetracycline Regulated Mouse Model of MYC-induced T-cell Acute Lymphoblastic Leukemia -- Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration and Tumourigenesis -- Detection of c-myc Amplification in Formalin-fixed Paraffin-Embedded Tumor Tissue by Chromogenic in situ Hybridization (CIHS) -- Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules.
Myc controls multiple cellular functions, including cell proliferation, growth, differentiation and death, both directly and indirectly, through its modulation of downstream transcriptional programs. In The Myc Gene: Methods and Protocols, experts in the field summarize the standard and novel techniques that allow the studying of Myc mechanism of action in normal and cancer cells, in vitro and in vivo, in one succinct manual. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Online resource; title from PDF title page (SpringerLink, viewed September 9, 2013).
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