Fluorescent protein-based biosensors : methods and protocols / edited by Jin Zhang and Qiang Ni, Robert H. Newman.

Contributor(s): Zhang, Jin (Molecular scientist) [editor.] | Ni, Qiang | Newman, Robert H
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1071.Publisher: New York. : Humana Press : Springer, ©2014Description: 1 online resource (xi, 251 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781627036221; 1627036229; 1627036210; 9781627036214Subject(s): Biosensors -- Laboratory manuals | Fluorescent probes -- Laboratory manuals | Molecular Probe Techniques | Investigative Techniques | Publication Formats | Biochemical Phenomena | Biology | Cell Physiological Phenomena | Proteins | Biological Science Disciplines | Publication Characteristics | Chemical Phenomena | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Natural Science Disciplines | Phenomena and Processes | Amino Acids, Peptides, and Proteins | Disciplines and Occupations | Chemicals and Drugs | Methods | Laboratory Manuals | Green Fluorescent Proteins | Signal Transduction | Biosensing Techniques | Genetics | Luminescent Proteins | Biosensors | Fluorescent probes | Biology | Health & Biological Sciences | Biomedical Engineering | Biophysics | eiwitten | proteins | levenswetenschappen | life sciences | celfysiologie | cell physiology | Proteins and Enzymes | Eiwitten en enzymenGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Fluorescent protein-based biosensors.DDC classification: 681/.25 LOC classification: R857.B54Online resources: Click here to access online
Contents:
Design and application of genetically encodable biosensors based on fluorescent proteins / Robert H. Newman and Jin Zhang -- Introduction to fluorescence imaging techniques geared towards biosensor applications / J. Goedhart, Mark A. Hink, and Kees Jalink -- Quantitative measurement of Ca² and Zn² in mammalian cells using genetically encoded fluorescent biosensors / J. Genevieve Park and Amy E. Palmer -- Detecting cAMP with an epac-based FRET sensor in single living cells / J. Klarenbeek and Kees Jalink -- Analysis of compartmentalized cAMP : a method to compare signals from differently targeted FRET reporters / Alessandra Stangherlin [and others] -- Genetically encoded fluorescent biosensors for live cell imaging of lipid dynamics / Moritoshi Sato -- Live-cell imaging of cytosolic NADH-NAD redox state using a genetically encoded fluorescent biosensor / Yin Pun Hung and Gary Yellen -- Measuring membrane voltage with microbial rhodopsins / Adam E. Cohen and Daniel R. Hochbaum -- Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons / Ana F. Oliveira and Ryohei Yasuda -- Imaging kinase activity at protein scaffolds / Maya T. Kunkel and Alexandra C. Newton -- Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells / Sohum Mehta and Jin Zhang -- Genetically encoded FRET indicators for live-cell imaging of histone acetylation / Kazuki Sasaki and Minoru Yoshida -- Genetically encoded fluorescent biosensors for live-cell imaging of MT1-MMP protease activity / Mingxing Ouyang, Shaoying Lu, and Yingxiao Wang -- Biosensor imaging in brain slice preparations / Marina Polito, Pierre Vincent, and Elvire Guiot -- Optical calcium imaging using DNA-encoded fluorescence sensors in transgenic fruit flies, Drosophila melanogaster / Shubham Dipt, Thomas Riemensperger, and André Fiala -- Multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase a dynamics in parallel / Nwe-Nwe Aye-Han and Jin Zhang -- FRET and BRET-based biosensors in live cell compound screens / Katie Herbst Robinson, Jessica R. Yang, and Jin Zhang -- Integrating fluorescent biosensor data using computational models / Eric C. Greenwald, Renata K. Polanowska-Grabowska, and Jeffrey J. Saucerman.
Summary: In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.
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Design and application of genetically encodable biosensors based on fluorescent proteins / Robert H. Newman and Jin Zhang -- Introduction to fluorescence imaging techniques geared towards biosensor applications / J. Goedhart, Mark A. Hink, and Kees Jalink -- Quantitative measurement of Ca² and Zn² in mammalian cells using genetically encoded fluorescent biosensors / J. Genevieve Park and Amy E. Palmer -- Detecting cAMP with an epac-based FRET sensor in single living cells / J. Klarenbeek and Kees Jalink -- Analysis of compartmentalized cAMP : a method to compare signals from differently targeted FRET reporters / Alessandra Stangherlin [and others] -- Genetically encoded fluorescent biosensors for live cell imaging of lipid dynamics / Moritoshi Sato -- Live-cell imaging of cytosolic NADH-NAD redox state using a genetically encoded fluorescent biosensor / Yin Pun Hung and Gary Yellen -- Measuring membrane voltage with microbial rhodopsins / Adam E. Cohen and Daniel R. Hochbaum -- Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons / Ana F. Oliveira and Ryohei Yasuda -- Imaging kinase activity at protein scaffolds / Maya T. Kunkel and Alexandra C. Newton -- Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells / Sohum Mehta and Jin Zhang -- Genetically encoded FRET indicators for live-cell imaging of histone acetylation / Kazuki Sasaki and Minoru Yoshida -- Genetically encoded fluorescent biosensors for live-cell imaging of MT1-MMP protease activity / Mingxing Ouyang, Shaoying Lu, and Yingxiao Wang -- Biosensor imaging in brain slice preparations / Marina Polito, Pierre Vincent, and Elvire Guiot -- Optical calcium imaging using DNA-encoded fluorescence sensors in transgenic fruit flies, Drosophila melanogaster / Shubham Dipt, Thomas Riemensperger, and André Fiala -- Multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase a dynamics in parallel / Nwe-Nwe Aye-Han and Jin Zhang -- FRET and BRET-based biosensors in live cell compound screens / Katie Herbst Robinson, Jessica R. Yang, and Jin Zhang -- Integrating fluorescent biosensor data using computational models / Eric C. Greenwald, Renata K. Polanowska-Grabowska, and Jeffrey J. Saucerman.

Includes bibliographical references and index.

In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction. Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.

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