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Methicillin-resistant Staphylococcus aureus (MRSA) protocols / edited by Yinduo Ji.

Contributor(s): Ji, YinduoMaterial type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1085.Publication details: New York : Humana Press : Springer, ©2014. Edition: 2nd edDescription: 1 online resource (x, 353 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781627036641; 1627036644; 9781627036634; 1627036636Other title: MRSA protocolsSubject(s): Staphylococcus aureus infections -- Laboratory manuals | Methicillin resistance -- Laboratory manuals | Therapeutics | Staphylococcus aureus | Gram-Positive Bacterial Infections | Epidemiologic Study Characteristics as Topic | Bacterial Infections | Health Care Evaluation Mechanisms | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Staphylococcus | Staphylococcaceae | Quality of Health Care | Bacterial Infections and Mycoses | Gram-Positive Cocci | Bacillales | Disease | Health Care Quality, Access, and Evaluation | Gram-Positive Endospore-Forming Rods | Gram-Positive Endospore-Forming Bacteria | Gram-Positive Bacteria | Delivery of Health Care | Bacteria | Endospore-Forming Bacteria | Organisms | Clinical Protocols | Staphylococcal Infections | Methicillin-Resistant Staphylococcus aureus | Methicillin resistance | Staphylococcus aureus infections | geneeskunde | medicine | bacteria | microbiologie | microbiology | bacteriologie | bacteriology | Bacteriology | BacteriologieGenre/Form: Laboratory Manuals. | Laboratory manuals. Additional physical formats: Printed edition:: No titleDDC classification: 616.9/297 LOC classification: QR201.S68 | M48 2014NLM classification: W1 | WC 250Online resources: Click here to access online
Contents:
Clinical, epidemiologic, and laboratory aspects of methicillin-resistant Staphylococcus aureus infections / Elizabeth L. Palavecino -- Community-associated methicillin-resistant Staphylococcus aureus case studies / Madeleine G. Sowash and Anne-Catrin Uhlemann -- Rapid methods for detection of MRSA in clinical specimens / Elizabeth L. Palavecino -- Immunofluorescence microscopy for the detection of surface antigens in methicillin-resistant Staphylococcus aureus (MRSA) / Yekaterina Timofeyeva, Ingrid L. Scully, and Annaliesa S. Anderson -- Internal transcribed spacer (ITS)-PCR identification of MRSA / Shin-ichi Fujita -- Pulsed-field gel electrophoresis typing of Staphylococcus aureus isolates / Yiping He, Yanping Xie, and Sue Reed -- Multilocus sequence typing (MLST) of Staphylococcus aureus / Nicholas A. Saunders and Anne Holmes -- Staphylococcal cassette chromosome mec (SCCmec) analysis of MRSA / Teruyo Ito [and others] -- Genetic interruption of target genes for investigation of virulence factors / Adhar C. Manna -- Molecular analysis of staphylococcal superantigens / Wilmara Salgado-Pabón, Laura C. Case-Cook, and Patrick M. Schlievert -- Investigation of Staphylococcus aureus adhesion and invasion of host cells / Junshu Yang and Yinduo Ji -- Investigation of biofilm formation in clinical Isolates of Staphylococcus aureus / James E. Cassat, Mark S. Smeltzer, and Chia Y. Lee -- Transcriptomic analysis of Staphylococcus aureus using microarray and advanced next-generation RNA-seq technologies / Ting Lei, Aaron Becker, and Yinduo Ji -- Proteomic approach to investigate pathogenicity and metabolism of methicillin-resistant Staphylococcus aureus / Patrice François [and others] -- Metabolomic investigation of methicillin-resistant Staphylococcus aureus / Ting Lei [and others] -- Treatment of infections due to resistant Staphylococcus aureus / Gregory M. Anstead, Jose Cadena, and Heta Javeri -- Anti-infective drug development for MRSA / Anu Daniel -- Animal models in drug development for MRSA / Andrea Marra.
Summary: Presenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S. aureus (MRSA), this second edition of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that utilize the power of complete genomic sequences and advanced high-throughput technologies that have pushed this field to its present state. These enable the development of specific and rapid diagnosis methods, the investigation and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identification of novel targets to develop more potent therapeutic and/or preventive agents. Written for the Methods in Molecular Biology series, numerous chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, Second Edition serves as a key reference for researchers attempting to set up a new method to study MRSA or even for technicians and scientists working on other pathogens.
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Clinical, epidemiologic, and laboratory aspects of methicillin-resistant Staphylococcus aureus infections / Elizabeth L. Palavecino -- Community-associated methicillin-resistant Staphylococcus aureus case studies / Madeleine G. Sowash and Anne-Catrin Uhlemann -- Rapid methods for detection of MRSA in clinical specimens / Elizabeth L. Palavecino -- Immunofluorescence microscopy for the detection of surface antigens in methicillin-resistant Staphylococcus aureus (MRSA) / Yekaterina Timofeyeva, Ingrid L. Scully, and Annaliesa S. Anderson -- Internal transcribed spacer (ITS)-PCR identification of MRSA / Shin-ichi Fujita -- Pulsed-field gel electrophoresis typing of Staphylococcus aureus isolates / Yiping He, Yanping Xie, and Sue Reed -- Multilocus sequence typing (MLST) of Staphylococcus aureus / Nicholas A. Saunders and Anne Holmes -- Staphylococcal cassette chromosome mec (SCCmec) analysis of MRSA / Teruyo Ito [and others] -- Genetic interruption of target genes for investigation of virulence factors / Adhar C. Manna -- Molecular analysis of staphylococcal superantigens / Wilmara Salgado-Pabón, Laura C. Case-Cook, and Patrick M. Schlievert -- Investigation of Staphylococcus aureus adhesion and invasion of host cells / Junshu Yang and Yinduo Ji -- Investigation of biofilm formation in clinical Isolates of Staphylococcus aureus / James E. Cassat, Mark S. Smeltzer, and Chia Y. Lee -- Transcriptomic analysis of Staphylococcus aureus using microarray and advanced next-generation RNA-seq technologies / Ting Lei, Aaron Becker, and Yinduo Ji -- Proteomic approach to investigate pathogenicity and metabolism of methicillin-resistant Staphylococcus aureus / Patrice François [and others] -- Metabolomic investigation of methicillin-resistant Staphylococcus aureus / Ting Lei [and others] -- Treatment of infections due to resistant Staphylococcus aureus / Gregory M. Anstead, Jose Cadena, and Heta Javeri -- Anti-infective drug development for MRSA / Anu Daniel -- Animal models in drug development for MRSA / Andrea Marra.

Includes bibliographical references and index.

Presenting the most up-to-date techniques for the detection, genotyping, and investigation of methicillin-resistant S. aureus (MRSA), this second edition of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that utilize the power of complete genomic sequences and advanced high-throughput technologies that have pushed this field to its present state. These enable the development of specific and rapid diagnosis methods, the investigation and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identification of novel targets to develop more potent therapeutic and/or preventive agents. Written for the Methods in Molecular Biology series, numerous chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, Second Edition serves as a key reference for researchers attempting to set up a new method to study MRSA or even for technicians and scientists working on other pathogens.

English.

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