Current laboratory methods in neuroscience research / Huangui Xiong, Howard E. Gendelman, editors.

Contributor(s): Xiong, Huangui [editor.] | Gendelman, Howard E, 1954- [editor.]
Material type: TextTextSeries: Springer protocols (Series): Publisher: New York, NY : Springer, 2014Description: 1 online resource (xiii, 585 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781461487944; 1461487943; 1461487935; 9781461487937Subject(s): Neurosciences -- Research | Neurosciences -- Laboratory manuals | Neurosciences | Research Design | MEDICAL -- Physiology | SCIENCE -- Life Sciences -- Human Anatomy & Physiology | Neurosciences | Neurosciences -- ResearchGenre/Form: Electronic books. | Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Printed edition:: No titleDDC classification: 612.8 LOC classification: RC337Online resources: Click here to access online
Contents:
Neurohistological Techniques -- Brain Tissue Preparation, Sectioning, and Staining / Jingdong Zhang and Huangui Xiong -- Brain Stereotaxic Injection / Jingdong Zhang and Huangui Xiong -- Neuronal Tract Tracing with Light and Electron Microscopy / Jingdong Zhang and Huangui Xiong -- Stereological Analysis / Kristi M. Anderson, Adam M. Szlachetka and R. Lee Mosley -- In Vitro Preparations -- Preparation and Use of Rodent Hippocampal Slices / Huangui Xiong and Jianxun Xia -- Single-Cell Neuronal Dissociation for Electrophysiological Studies / Yu-Long Li -- Isolation and Culture of Human Neurons, Microglia, and Astrocytes / Li Wu and Santhi Gorantla -- Isolation and Culture of Neural Stem/Progenitor Cells / Hui Peng, Qiang Chen and Jialin Zheng -- Isolation of Mitochondria from Brain Tissue and Cells / Changhai Tian and Jialin Zheng -- Isolation of Synaptosomes from Archived Brain Tissues / Gurudutt Pendyala, James L. Buescher and Howard S. Fox -- Xenopus Oocytes Isolation and Microinjection / Huangui Xiong -- Leukocyte Isolation and Application in Neuroscience -- Centrifugal Elutriation for Studies of Neuroimmunity / Santhi Gorantla, Myhanh Che and Howard E. Gendelman -- Flow Cytometry in Neuroscience Research / Charles Kuszynski -- Standard Laboratory Nucleic Acid and Protein Detections -- Western Blotting Technique in Biomedical Research / Jianuo Liu, James Haorah and Huangui Xiong -- Polymerase Chain Reaction (PCR) and Real-Time PCR / Georgette D. Kanmogne -- Nanomedicine -- Nanoformulations / Xin-Ming Liu and Tatiana K. Bronich -- Neuronanomedicine / JoEllyn M. McMillan, Xin-Ming Liu and Howard E. Gendelman -- Bioimaging -- Confocal Imaging of Nerve Cells / You Zhou -- Magnetic Resonance Imaging / Yutong Liu -- X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging / Katherine A. Estes, Jacob C. Peterson, Adam M. Szlachetka and R. Lee Mosley -- Noninvasive Neurophysiological Imaging with Magnetoencephalography / Tony W. Wilson -- Multiphoton Brain Imaging / Anna Dunaevsky -- Neuroelectrophysiology -- Techniques for Extracellular Recordings / Jingdong Zhang, Jianxun Xia and Huangui Xiong -- Blind Patch-Clamp Recordings from Rodent Hippocampal Slices / Jianxun Xia and Huangui Xiong -- Whole-Cell Patch-Clamp Recording / Matthew J. Van Hook and Wallace B. Thoreson -- Immunohistochemistry and Autoradiography -- Analysis of Receptor Binding and Quantitative Autoradiography / L. Charles Murrin -- Techniques in Immunohistochemistry and Immunocytochemistry / Yang Yuan and Jyothi Arikkath -- Analysis of Gene Expression -- Protein Identification by Mass Spectrometry: Proteomics / Melinda Wojtkiewicz, Kelley Barnett and Pawel Ciborowski -- DNA Microarrays as a Tool for Neurosciences Research / James D. Eudy, Lynette Smith -- Metabolomics / Yazen Alnouti -- Bioinformatic Methods and Resources for Neuroscience Research / Chittibabu Guda -- Animal Models: Behavior and Pathology -- Motor Function in Rodent Models of Neurodegenerative Disorders / Jessica A. Hutter Saunders, Max V. Kuenstling, Robert A. Weir, R. Lee Mosley and Howard E. Gendelman -- Humanized Mice / Larisa Y. Poluektova and Edward Makarov -- Animal Models for PD and ALS / Max V. Kuenstling, Adam M. Szlachetka and R. Lee Mosley -- Animal Models of Alzheimer's Disease / Tomomi Kiyota -- Animal Models: Behavior and Pathology: Preclinical Assessment of the Putative Cognitive Deficits in HAND / Landhing M. Moran, Rosemarie M. Booze and Charles F. Mactutus.
Summary: Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a blueprint for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.
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Part I. Neurohistological Techniques -- Brain Tissue Preparation, Sectioning, and Staining / Jingdong Zhang and Huangui Xiong -- Brain Stereotaxic Injection / Jingdong Zhang and Huangui Xiong -- Neuronal Tract Tracing with Light and Electron Microscopy / Jingdong Zhang and Huangui Xiong -- Stereological Analysis / Kristi M. Anderson, Adam M. Szlachetka and R. Lee Mosley -- Part II. In Vitro Preparations -- Preparation and Use of Rodent Hippocampal Slices / Huangui Xiong and Jianxun Xia -- Single-Cell Neuronal Dissociation for Electrophysiological Studies / Yu-Long Li -- Isolation and Culture of Human Neurons, Microglia, and Astrocytes / Li Wu and Santhi Gorantla -- Isolation and Culture of Neural Stem/Progenitor Cells / Hui Peng, Qiang Chen and Jialin Zheng -- Isolation of Mitochondria from Brain Tissue and Cells / Changhai Tian and Jialin Zheng -- Isolation of Synaptosomes from Archived Brain Tissues / Gurudutt Pendyala, James L. Buescher and Howard S. Fox -- Xenopus Oocytes Isolation and Microinjection / Huangui Xiong -- Part III. Leukocyte Isolation and Application in Neuroscience -- Centrifugal Elutriation for Studies of Neuroimmunity / Santhi Gorantla, Myhanh Che and Howard E. Gendelman -- Flow Cytometry in Neuroscience Research / Charles Kuszynski -- Part IV. Standard Laboratory Nucleic Acid and Protein Detections -- Western Blotting Technique in Biomedical Research / Jianuo Liu, James Haorah and Huangui Xiong -- Polymerase Chain Reaction (PCR) and Real-Time PCR / Georgette D. Kanmogne -- Part V. Nanomedicine -- Nanoformulations / Xin-Ming Liu and Tatiana K. Bronich -- Neuronanomedicine / JoEllyn M. McMillan, Xin-Ming Liu and Howard E. Gendelman -- Part VI. Bioimaging -- Confocal Imaging of Nerve Cells / You Zhou -- Magnetic Resonance Imaging / Yutong Liu -- X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging / Katherine A. Estes, Jacob C. Peterson, Adam M. Szlachetka and R. Lee Mosley -- Noninvasive Neurophysiological Imaging with Magnetoencephalography / Tony W. Wilson -- Multiphoton Brain Imaging / Anna Dunaevsky -- Part VII. Neuroelectrophysiology -- Techniques for Extracellular Recordings / Jingdong Zhang, Jianxun Xia and Huangui Xiong -- Blind Patch-Clamp Recordings from Rodent Hippocampal Slices / Jianxun Xia and Huangui Xiong -- Whole-Cell Patch-Clamp Recording / Matthew J. Van Hook and Wallace B. Thoreson -- Part VIII. Immunohistochemistry and Autoradiography -- Analysis of Receptor Binding and Quantitative Autoradiography / L. Charles Murrin -- Techniques in Immunohistochemistry and Immunocytochemistry / Yang Yuan and Jyothi Arikkath -- Part IX. Analysis of Gene Expression -- Protein Identification by Mass Spectrometry: Proteomics / Melinda Wojtkiewicz, Kelley Barnett and Pawel Ciborowski -- DNA Microarrays as a Tool for Neurosciences Research / James D. Eudy, Lynette Smith -- Metabolomics / Yazen Alnouti -- Bioinformatic Methods and Resources for Neuroscience Research / Chittibabu Guda -- Part X. Animal Models: Behavior and Pathology -- Motor Function in Rodent Models of Neurodegenerative Disorders / Jessica A. Hutter Saunders, Max V. Kuenstling, Robert A. Weir, R. Lee Mosley and Howard E. Gendelman -- Humanized Mice / Larisa Y. Poluektova and Edward Makarov -- Animal Models for PD and ALS / Max V. Kuenstling, Adam M. Szlachetka and R. Lee Mosley -- Animal Models of Alzheimer's Disease / Tomomi Kiyota -- Animal Models: Behavior and Pathology: Preclinical Assessment of the Putative Cognitive Deficits in HAND / Landhing M. Moran, Rosemarie M. Booze and Charles F. Mactutus.

Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a blueprint for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.

Online resource; title from PDF title page (SpringerLink, viewed November 11, 2013).

Includes bibliographical references and index.

English.

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