Structural genomics : general applications / edited by Yu Wai Chen, Randall Division of Cell & Molecular Biophysics, King's College London, London, UK.
Contributor(s): Chen, Yu Wai [editor.]Material type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 1091.Publisher: New York : Humana Press, 2014Description: 1 online resource (xv, 358 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781627036917; 1627036911; 1627036903; 9781627036900Subject(s): Genomics -- Laboratory manuals | Proteins | Genetics | Computational Biology | Biological Science Disciplines | Investigative Techniques | Molecular Conformation | Amino Acids, Peptides, and Proteins | Molecular Structure | Natural Science Disciplines | Biology | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Chemicals and Drugs | Disciplines and Occupations | Biochemical Phenomena | Chemical Phenomena | Phenomena and Processes | Proteins | Genomics | Methods | Physiology | Protein Conformation | Genomics | ProteinsGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Structural genomics.DDC classification: 576.5 LOC classification: QH440.5QP551Online resources: Click here to access online
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Includes bibliographical references and index.
DisMeta : a meta server for construct design and optimization / Yuanpeng Janet Huang, Thomas B. Acton, and Gaetano T. Montelione -- Stable expression clones and auto-induction for protein production in E. coli / F. William Studier -- High-throughput expression screening and purification of recombinant proteins in E. coli / Natalie J. Saez and Renaud Vincentelli -- Medium-throughput production of recombinant human proteins : ligation-independent cloning / Claire Strain-Damerell [and others] -- Medium-throughput production of recombinant human proteins : protein production in E. coli / Nicola A. Burgess-Brown [and others] -- Medium-throughput production of recombinant human proteins : protein production in insect cells / Pravin Mahajan [and others] -- OmniBac : universal multigene transfer plasmids for baculovirus expression vectors systems / Deepak B. Thimiri Govinda Raj, Lakshmi S. Vijayachandran, and Imre Berger -- Multiprotein complex production in insect cells by using polyproteins / Yan Nie [and others] -- Expression screening in mammalian suspension cells / Susan D. Chapple and Michael R. Dyson -- Cell-free expression of protein complexes for structural biology / Takaho Terada [and others] -- Cell-free protein synthesis for functional and structural studies / Shin-ichi Makino [and others] -- Insoluble protein purification with sarkosyl : facts and precautions / Ben Chisnall [and others] -- Estimation of crystallization likelihood through a fluorimetric thermal stability assay / Vincent Mariaule, Florine Dupeux, and José A. Márquez -- CrystalDirect : a novel approach for automated crystal harvesting based on photoablation of thin films / José A. Márquez and Florent Cipriani -- Methods to refine macromolecular structures in cases of severe diffraction anisotropy / Michael R. Sawaya -- Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination / Yunhuang Yang [and others] -- Cost-effective protocol for the parallel production of libraries of 13CH3-specifically labeled mutants for NMR studies of high molecular weight proteins / Elodie Crublet [and others] -- High-throughput SAXS for the characterization of biomolecules in solution : a practical approach / Kevin N. Dyer [and others] -- Measuring spatial restraints on native protein complexes using isotope-tagged chemical cross-linking and mass spectrometry / Franz Herzog -- Modeling of proteins and their assemblies with the integrative modeling platform / Benjamin Webb [and others] -- Quality and validation of structures from structural genomics / Marcin J. Domagalski [and others] -- Navigating the global protein-protein interaction landscape using iRefWeb / Andrei L. Turinsky [and others] -- Mespeus-a database of metal interactions with proteins / Marjorie M. Harding and Kun-Yi Hsin -- High-quality macromolecular graphics on mobile devices : a quick starter's guide / Chin-Pang Benny Yiu and Yu Wai Chen.
The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.
Online resource; title from PDF title page (SpringerLink, viewed November 11, 2013).