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Plant metabolism : methods and protocols / edited by Ganesh Sriram, Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD, USA.

Contributor(s): Sriram, Ganesh [editor.]Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1083.Publisher: New York : Humana Press, [2014]Copyright date: ©2014Description: 1 online resource (x, 318 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781627036610; 162703661X; 1627036601; 9781627036603Subject(s): Plants -- Metabolism -- Laboratory manuals | Metabolism | Eukaryota | Publication Formats | Organisms | Phenomena and Processes | Publication Characteristics | Laboratory Manuals | Plants | Plants -- MetabolismGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Plant metabolism.DDC classification: 572/.42 LOC classification: QK881NLM classification: W1 | QK 881Online resources: Click here to access online
Contents:
MeRy-B, a metabolomic database and knowledge base for exploring plant primary metabolism / Catherine Deborde and Daniel Jacob -- Targeted deuteration of polyphenolics for their qualitative and quantitative metabolomic analysis in plant-derived extracts / Mikel R. Roe, Jerry D. Cohen, and Adrian D. Hegeman -- Relative quantitation in single-cell metabolomics by laser ablation electrospray mass spectrometry / Bindesh Shrestha and Akos Vertes -- Quantification of plant volatiles / Anthony V. Qualley and Natalia Dudareva -- Quantitative imaging approaches for small-molecule measurements using FRET sensors in plants / Sakiko Okumoto -- Isotopomer measurement techniques in metabolic flux analysis I : nuclear magnetic resonance / Quyen X. Truong, Jong Moon Yoon, and Jacqueline V. Shanks -- Isotopomer measurement techniques in metabolic flux analysis II : mass spectrometry / Jamey D. Young, Douglas K. Allen, and John A. Morgan -- Mathematical modeling of isotope labeling experiments for metabolic flux analysis / Shilpa Nargund and Ganesh Sriram -- Optimal design of isotope labeling experiments / Hong Yang, Dominic E. Mandy, and Igor G.L. Libourel -- Putting the plant metabolic network pathway databases to work : going offline to gain new capabilities / Kate Dreher -- Elucidation of metabolic pathways from enzyme classification data / Andrew G. McDonald and Keith F. Tipton -- Deducing intracellular distributions of metabolic pathways from genomic data / Ansgar Gruber and Peter G. Kroth -- Genome-scale models of plant metabolism / Margaret Simons, Ashish Misra, and Ganesh Sriram -- Elementary flux modes, flux balance analysis, and their application to plant metabolim / Katrin Lotz [and four others] -- Systems approaches to unraveling plant metabolism : identifying biosynthetic genes of secondary metabolic pathways / Martin J. Spiering [and three others] -- Applications of kinetic modeling to plant metabolism / Johann M. Rohwer -- Kinetic modeling of plant metabolism and its predictive power : peppermint essential oil biosynthesis as an example / Bernd Markus Lange and Rigoberto Rios-Estepa.
Summary: In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.
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Includes bibliographical references and index.

MeRy-B, a metabolomic database and knowledge base for exploring plant primary metabolism / Catherine Deborde and Daniel Jacob -- Targeted deuteration of polyphenolics for their qualitative and quantitative metabolomic analysis in plant-derived extracts / Mikel R. Roe, Jerry D. Cohen, and Adrian D. Hegeman -- Relative quantitation in single-cell metabolomics by laser ablation electrospray mass spectrometry / Bindesh Shrestha and Akos Vertes -- Quantification of plant volatiles / Anthony V. Qualley and Natalia Dudareva -- Quantitative imaging approaches for small-molecule measurements using FRET sensors in plants / Sakiko Okumoto -- Isotopomer measurement techniques in metabolic flux analysis I : nuclear magnetic resonance / Quyen X. Truong, Jong Moon Yoon, and Jacqueline V. Shanks -- Isotopomer measurement techniques in metabolic flux analysis II : mass spectrometry / Jamey D. Young, Douglas K. Allen, and John A. Morgan -- Mathematical modeling of isotope labeling experiments for metabolic flux analysis / Shilpa Nargund and Ganesh Sriram -- Optimal design of isotope labeling experiments / Hong Yang, Dominic E. Mandy, and Igor G.L. Libourel -- Putting the plant metabolic network pathway databases to work : going offline to gain new capabilities / Kate Dreher -- Elucidation of metabolic pathways from enzyme classification data / Andrew G. McDonald and Keith F. Tipton -- Deducing intracellular distributions of metabolic pathways from genomic data / Ansgar Gruber and Peter G. Kroth -- Genome-scale models of plant metabolism / Margaret Simons, Ashish Misra, and Ganesh Sriram -- Elementary flux modes, flux balance analysis, and their application to plant metabolim / Katrin Lotz [and four others] -- Systems approaches to unraveling plant metabolism : identifying biosynthetic genes of secondary metabolic pathways / Martin J. Spiering [and three others] -- Applications of kinetic modeling to plant metabolism / Johann M. Rohwer -- Kinetic modeling of plant metabolism and its predictive power : peppermint essential oil biosynthesis as an example / Bernd Markus Lange and Rigoberto Rios-Estepa.

In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.

Online resource; title from PDF title page (SpringerLink, viewed November 18, 2013).

English.

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