Brain energy metabolism / edited by Johannes Hirrlinger, Helle S. Waagepetersen.

Contributor(s): Hirrlinger, Johannes [editor.] | Waagepetersen, Helle S [editor.]
Material type: TextTextSeries: Neuromethods: 90.Publisher: New York, NY : Humana Press, 2014Description: 1 online resource (xiv, 368 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493910595; 1493910590; 1493910582; 9781493910588Subject(s): Brain -- Metabolism | Energy metabolism | Brain -- metabolism | Brain Chemistry -- physiology | Energy Metabolism | Brain -- Metabolism | Energy metabolism | biomedische wetenschappen | biomedicine | neurowetenschap | neuroscience | neurobiologie | neurobiology | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Brain energy metabolism.DDC classification: 612.8/042 LOC classification: QP376Online resources: Click here to access online
Contents:
Determination of CO2 Production in Subcellular Preparations like Synaptosomes and Isolated Mitochondria Using 14C-Labeled Substrates and Radioactive CO2 Measurements -- Transport of Lactate: Characterization of the Transporters Involved in Transport at the Plasma Membrane by Heterologous Protein Expression in Xenopus Oocytes -- Primary Cultures of Astrocytes and Neurons as Model Systems to Study the Metabolism and Metabolite Export from Brain Cells -- Metabolic Mapping of Astrocytes and Neurons in Culture Using Stable Isotopes and Gas Chromatography-Mass Spectrometry (GC-MS) -- Metabolic Flux Analysis Tools to Investigate Brain Metabolism In Vitro -- Fluorescent Nanosensor Based Flux Analysis: Overview and the Example of Glucose -- Mitochondrial Bioenergetics Assessed by Functional Fluorescence Dyes -- RNA Interference as a Tool to Selectively Down-Modulate Protein Function -- Localizing and Quantifying Metabolites In Situ with Luminometry: Induced Metabolic Bioluminescence Imaging (imBI) -- A Chip Off the Old Block: The Brain Slice as a Model for Metabolic Studies of Brain Compartmentation and Neuropharmacology -- Integrated Measurements of Electrical Activity, Oxygen Tension, Blood Flow, and Ca2+-Signaling in Rodents In Vivo -- Measuring Cerebral Hemodynamics and Energy Metabolism by Near-Infrared Spectroscopy -- Compartmental Analysis of Metabolism by 13C Magnetic Resonance Spectroscopy -- Positron Emission Tomography of Brain Glucose Metabolism with [18F]Fluorodeoxyglucose in Humans.
Summary: Brain Energy Metabolism addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity. Model systems are discussed, starting from the reductionist approach like primary cell cultures which allow assessing of the properties and functions of a single brain cell type with many different types of analysis, however, at the expense of neglecting the interaction between cell types in the brain. On the other end, analysis in animals and humans in vivo is discussed, maintaining the full complexity of the tissue and the organism but making high demands on the methods of analysis. Written for the popular Neuromethods series, chapters include the kind of detailed description and key implementation advice that aims to support reproducible results in the lab. Meticulous and authoritative, Brain Energy Metabolism provides an ideal guide for researchers interested in brain energy metabolism with the hope of stimulating more research in this exciting and very important field.
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Includes index.

Online resource; title from PDF title page (SpringerLink, viewed October 2, 2014).

Determination of CO2 Production in Subcellular Preparations like Synaptosomes and Isolated Mitochondria Using 14C-Labeled Substrates and Radioactive CO2 Measurements -- Transport of Lactate: Characterization of the Transporters Involved in Transport at the Plasma Membrane by Heterologous Protein Expression in Xenopus Oocytes -- Primary Cultures of Astrocytes and Neurons as Model Systems to Study the Metabolism and Metabolite Export from Brain Cells -- Metabolic Mapping of Astrocytes and Neurons in Culture Using Stable Isotopes and Gas Chromatography-Mass Spectrometry (GC-MS) -- Metabolic Flux Analysis Tools to Investigate Brain Metabolism In Vitro -- Fluorescent Nanosensor Based Flux Analysis: Overview and the Example of Glucose -- Mitochondrial Bioenergetics Assessed by Functional Fluorescence Dyes -- RNA Interference as a Tool to Selectively Down-Modulate Protein Function -- Localizing and Quantifying Metabolites In Situ with Luminometry: Induced Metabolic Bioluminescence Imaging (imBI) -- A Chip Off the Old Block: The Brain Slice as a Model for Metabolic Studies of Brain Compartmentation and Neuropharmacology -- Integrated Measurements of Electrical Activity, Oxygen Tension, Blood Flow, and Ca2+-Signaling in Rodents In Vivo -- Measuring Cerebral Hemodynamics and Energy Metabolism by Near-Infrared Spectroscopy -- Compartmental Analysis of Metabolism by 13C Magnetic Resonance Spectroscopy -- Positron Emission Tomography of Brain Glucose Metabolism with [18F]Fluorodeoxyglucose in Humans.

Brain Energy Metabolism addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity. Model systems are discussed, starting from the reductionist approach like primary cell cultures which allow assessing of the properties and functions of a single brain cell type with many different types of analysis, however, at the expense of neglecting the interaction between cell types in the brain. On the other end, analysis in animals and humans in vivo is discussed, maintaining the full complexity of the tissue and the organism but making high demands on the methods of analysis. Written for the popular Neuromethods series, chapters include the kind of detailed description and key implementation advice that aims to support reproducible results in the lab. Meticulous and authoritative, Brain Energy Metabolism provides an ideal guide for researchers interested in brain energy metabolism with the hope of stimulating more research in this exciting and very important field.

Includes bibliographical references and index.

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